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Physiological and morphological characterization of GABAergic neurons in the medial amygdala
Bian, Xiling1,2
关键词Medial amygdala Intrinsic properties Whole-cell patch-clamp recording Slice Morphology
刊名BRAIN RESEARCH
2013-05-06
DOI10.1016/j.brainres.2013.03.012
1509页:8-19
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Neurosciences
研究领域[WOS]Neurosciences & Neurology
关键词[WOS]ACCESSORY OLFACTORY-BULB ; EXTENDED AMYGDALA ; SOCIAL RECOGNITION ; CONDITIONED FEAR ; MALE GERBILS ; RAT ; CELLS ; PARVALBUMIN ; OXYTOCIN ; BEHAVIOR
英文摘要

GABAergic neurons in the medial amygdala (MeA) have been indicated in information processing in reproductive behavior and fear/anxiety. However, basic knowledge of their physiological and morphological properties is still very limited, probably due to the technical challenge to selectively record the GABAergic neurons. In this study, I characterized properties of the MeA GABAergic neurons by performing whole-cell patch clamp recordings from brain slices of adult knock-in mice selectively expressing green fluorescence protein (GFP) in GABAergic neurons. The majority (73%) of GABAergic neurons exhibiting low threshold calcium spike were classified as type I neurons, with morphological properties of being bitufted or stellate, and dendrites either aspiny or covered by various shapes of spines. Axonal collaterals of some neurons were observed near somata as well as in other amygdaloid nuclei. Neurons incapable of generate low threshold calcium spikes were divided into two types. Type II neurons (11%) exhibited hyperpolarization-activated sag and higher input resistance (>400 M Omega). Most Type II neurons exhibited asymmetric dendritic trees extending towards the superficial layer covered with long neck dendritic spines. The axons of type II neurons formed large collaterals and projected to other amygdaloid nuclei. Type III neurons (16%) lack prominent hyperpolarization-activated sag and possessed lower input resistance (<400 M Omega). These neurons were local interneurons with smooth multipolar dendritic trees. Since both MeA and nearby amygdaloid nuclei are involved in fear/anxiety processing, two types of MeA GABAergic projection neurons and a third type of interneurons that might participate in anxiety-related behavior were revealed by my present study. (C) 2013 Elsevier B.V. All rights reserved.

语种英语
WOS记录号WOS:000319093400002
项目编号81000552
资助机构China Ministry of Science and Technology ; Human Frontier Science Program ; Natural Science Foundation of China
引用统计
被引频次:8[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/60939
专题北京大学药学院
北京大学药学院_分子与细胞药理学系
作者单位1.Peking Univ, Sch Pharmaceut Sci, Dept Mol & Cellular Pharmacol, Hlth Sci Ctr, Beijing 100191, Peoples R China
2.Natl Inst Biol Sci, Beijing 102206, Peoples R China
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GB/T 7714
Bian, Xiling. Physiological and morphological characterization of GABAergic neurons in the medial amygdala[J]. BRAIN RESEARCH,2013,1509:8-19.
APA Bian, Xiling.(2013).Physiological and morphological characterization of GABAergic neurons in the medial amygdala.BRAIN RESEARCH,1509,8-19.
MLA Bian, Xiling."Physiological and morphological characterization of GABAergic neurons in the medial amygdala".BRAIN RESEARCH 1509(2013):8-19.
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