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学科主题临床医学
DNA Copy-number Loss on 1p36.1 Harboring RUNX3 with Promoter Hypermethylation and Associated Loss of RUNX3 Expression in Liver Fluke-associated Intrahepatic Cholangiocarcinoma
Dachrut, Somkid2,3,4; Banthaisong, S.2; Sripa, M.2; Paeyao, A.3; Ho, C.1; Lee, S. A.1; Kosinski, C.1; Patil, M. A.1; Zhang, J.5; Chen, X.1; Sripa, Banchob2,3; Pairojkul, Chawalit2,3
关键词RUNX3 DNA hypermethylation chromosome 1p36 microarray comparative genomic hybridization
刊名ASIAN PACIFIC JOURNAL OF CANCER PREVENTION
2009
10期:4页:575-582
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Oncology
资助者Commission on Higher Education ; Ministry of Education of Thailand and the Division of Research Affairs ; Faculty of Medicine, Khon Kaen University ; Commission on Higher Education ; Ministry of Education of Thailand and the Division of Research Affairs ; Faculty of Medicine, Khon Kaen University
研究领域[WOS]Oncology
关键词[WOS]CANCER-CELL-LINES ; TUMOR-SUPPRESSOR GENE ; GASTRIC EPITHELIAL-CELLS ; TGF-BETA SUPERFAMILY ; ARRAY CGH ANALYSIS ; DOWN-REGULATION ; HEPATOCELLULAR-CARCINOMA ; HEMIZYGOTIC DELETION ; PANCREATIC-CANCER ; BREAST-CANCER
英文摘要

Runt-related transcription factor 3 (RUNX3) is a candidate tumor suppressor gene, localized on 1p36, involved in TGF-beta-Smads signaling. To assess its role in liver fluke-associated intrahepatic cholangiocarcinoma (ICC), the promoter methylation status was investigated in 53 ICCs by methylation-specific PCR, with determination of loss of 1p36.1 by microarray comparative genomic hybridization and RUNX3 protein expression by immunohistochemistry. Loss at 1p36.1 was found 41.5% of ICCs (22/53). In addition, DNA hypermethylation of the RUNX3 promoter was found in 49.1% (26/53) of cancers and 57.1% (4/7) of ICC cell lines. The protein was highly expressed in normal bile ducts but mostly decreased in ICCs, 67.9% (n=36) being negative for immunohistochemical staining. Promoter hypermethylation of RUNX3 was associated with reversible decrease or absence of RUNX3 protein expression (p<0.001), but this was not found to differ with the ICC subtype. In contrast, loss of 1p36.1 demonstrated a significant link (p=0.020). In conclusion, RUNX3 promoter hypermethylation and loss of 1p36.1 are causal mechanisms for loss of RUNX3 function in liver fluke-associated ICC carcinogenesis.

语种英语
所属项目编号KKU5103200017 (I51117)
资助者Commission on Higher Education ; Ministry of Education of Thailand and the Division of Research Affairs ; Faculty of Medicine, Khon Kaen University ; Commission on Higher Education ; Ministry of Education of Thailand and the Division of Research Affairs ; Faculty of Medicine, Khon Kaen University
WOS记录号WOS:000270819700009
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被引频次:11[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/60940
专题北京大学临床肿瘤学院
作者单位1.Univ Calif San Francisco, Dept Biopharmaceut Sci, San Francisco, CA 94143 USA
2.Khon Kaen Univ, Fac Med, Liver Fluke & Cholangiocarcinoma Res Ctr, Khon Kaen, Thailand
3.Khon Kaen Univ, Fac Med, Dept Pathol, Div Expt Pathol, Khon Kaen, Thailand
4.Walailuk Univ, Inst Allied Hlth Sci & Publ Hlth, Nakhon Si Thammarat, Thailand
5.Peking Univ, Beijing Canc Hosp, Sch Oncol, Dept Surg, Beijing 100871, Peoples R China
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Dachrut, Somkid,Banthaisong, S.,Sripa, M.,et al. DNA Copy-number Loss on 1p36.1 Harboring RUNX3 with Promoter Hypermethylation and Associated Loss of RUNX3 Expression in Liver Fluke-associated Intrahepatic Cholangiocarcinoma[J]. ASIAN PACIFIC JOURNAL OF CANCER PREVENTION,2009,10(4):575-582.
APA Dachrut, Somkid.,Banthaisong, S..,Sripa, M..,Paeyao, A..,Ho, C..,...&Pairojkul, Chawalit.(2009).DNA Copy-number Loss on 1p36.1 Harboring RUNX3 with Promoter Hypermethylation and Associated Loss of RUNX3 Expression in Liver Fluke-associated Intrahepatic Cholangiocarcinoma.ASIAN PACIFIC JOURNAL OF CANCER PREVENTION,10(4),575-582.
MLA Dachrut, Somkid,et al."DNA Copy-number Loss on 1p36.1 Harboring RUNX3 with Promoter Hypermethylation and Associated Loss of RUNX3 Expression in Liver Fluke-associated Intrahepatic Cholangiocarcinoma".ASIAN PACIFIC JOURNAL OF CANCER PREVENTION 10.4(2009):575-582.
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