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学科主题: 公共卫生
题名:
In vitro effect of manganese chloride exposure on reactive oxygen species generation and respiratory chain complexes activities of mitochondria isolated from rat brain
作者: Zhang, SR; Fu, JL; Zhou, ZC
关键词: manganese chloride (MnCl2) ; mitochondria ; reactive oxygen species ; respiratory chain complexes ; free radicals ; in vitro ; antioxidants ; membrane potential
刊名: TOXICOLOGY IN VITRO
发表日期: 2004-02-01
DOI: 10.1016/j.tiv.2003.09.002
卷: 18, 期:1, 页:71-77
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Toxicology
研究领域[WOS]: Toxicology
关键词[WOS]: CELLULAR DEFENSE-MECHANISMS ; OXIDATIVE STRESS ; SUPEROXIDE DISMUTASE ; LIVER-MITOCHONDRIA ; STRIATUM ; VULNERABILITY ; NEUROTOXICITY ; DISEASES ; TOXICITY ; ETHANOL
英文摘要:

Manganese (Mn) is known to induce mitochondrial dysfunction in excessive dose; however the mechanisms underlying its action are not elucidated clearly. To determine if Mn2+ can act directly on mitochondria or indirectly by producing reactive oxygen species (ROS), isolated mitochondria were exposed to different concentration of Mn 21 (5, 50, 500, 1000 muM). ROS generation, respiratory control ratio (RCR), mitochondrial membrane potential (MMP) and respiratory chain complexes activities were investigated. Dose-dependent inhibition of respiratory chain complexes and induction of ROS were observed; these changes were paralleled by decreasing of respiratory control ratio (RCR) both with succinate or glutamate + malate. Further investigation indicated that the membrane potential determined by Rhodamine123 release decreased after MnCl2 exposure at 1000 muM. In addition, effects of the antioxidants NAC (500 muM), GSH (500 muM) and Vitamin C (500 muM) were studied at 500 muM Mn2+. The results indicate that the effect of Mn2+ exposure on respiratory chain is not site-specific, and antioxidants can protect the mitochondria function by reducing the formation of free radicals. (C) 2003 Elsevier Ltd. All rights reserved.

语种: 英语
WOS记录号: WOS:000188058900009
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/60955
Appears in Collections:北京大学公共卫生学院_毒理学系_期刊论文

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作者单位: Peking Univ, Sch Publ Hlth, Hlth Sci Ctr, Dept Toxicol, Beijing 100083, Peoples R China

Recommended Citation:
Zhang, SR,Fu, JL,Zhou, ZC. In vitro effect of manganese chloride exposure on reactive oxygen species generation and respiratory chain complexes activities of mitochondria isolated from rat brain[J]. TOXICOLOGY IN VITRO,2004,18(1):71-77.
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