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学科主题: 临床医学
题名:
Apelin in epiretinal membranes of patients with proliferative diabetic retinopathy
作者: Lu, Qiang1,2; Ma, Yan1,3; Xu, Yong-sheng1,4; Jiang, Yan-rong1
刊名: MOLECULAR VISION
发表日期: 2014-07-31
卷: 20, 页:1122-1131
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Ophthalmology
研究领域[WOS]: Biochemistry & Molecular Biology ; Ophthalmology
关键词[WOS]: ENDOTHELIAL GROWTH-FACTOR ; INTRAVITREAL BEVACIZUMAB AVASTIN ; PI3K/AKT/ENOS-DEPENDENT SIGNAL PATHWAYS ; RETINAL DEGENERATION ; STIMULATES ANGIOGENESIS ; VASCULAR DEVELOPMENT ; CELL-PROLIFERATION ; PEPTIDE APELIN ; NITRIC-OXIDE ; EXPRESSION
英文摘要:

Purpose: Formation of epiretinal membranes (ERMs) in the posterior fundus results in visual impairment. ERMs have been associated with numerous clinical conditions, including proliferative diabetic retinopathy (PDR), a neovascular disease. Apelin has been identified as a novel angiogenesis contributor. The aim of this study was to investigate the correlation between apelin and ERMs after PDR.

Methods: ERM samples were obtained by vitrectomy from 12 subjects with PDR (aged 57 +/- 6 years; duration of diabetes 16 +/- 7 years), and 12 subjects with idiopathic ERM (aged 68 +/- 5 years). The samples were processed for immunohistochemistry and reverse transcription-PCR (RT-PCR). We also analyzed samples from patients with PDR who received an intravitreal injection of bevacizumab (IVB) before vitrectomy.

Results: The mRNA expression of apelin was significantly higher in the PDR ERMs than in the idiopathic ERMs. Accordingly, immunohistochemical analysis revealed strong expression of apelin in all eight PDR ERMs without IVB, and was double-labeled with glial fibrillary acidic protein antibody (GFAP), platelet endothelial cell adhesion molecule-1 (CD31), cytokeratin (CK) and vascular endothelial growth factor (VEGF) but not with fibronectin. They were mainly located in the adventitia. In contrast, the expression of apelin was lower in the PDR ERMs after IVB and the idiopathic ERMs.

Conclusions: The results showed that apelin was involved in the formation of ERMs and promoted the formation of adventitia, including glial, endothelial, and RPE cells. Bevacizumab blocked the expression of apelin and regressed gliosis and angiogenesis.

语种: 英语
所属项目编号: 81271027 ; 81260152 ; 2127000043
项目资助者: National Natural Science Foundation of China ; EFSD/CDS/Lilly grant
WOS记录号: WOS:000341972700005
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/60966
Appears in Collections:北京大学第二临床医学院_眼科_期刊论文

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作者单位: 1.Inner Mongolia Peoples Hosp, Dept Ophthalmol, Hohhot, Peoples R China
2.Peking Univ, Peoples Hosp, Dept Ophthalmol, Key Lab Vis Loss & Restorat,Minist Educ,Beijing K, Beijing 100871, Peoples R China
3.Capital Med Univ, Beijing Tongren Hosp, Beijing Tongren Eye Ctr, Dept Ophthalmol, Beijing, Peoples R China
4.Peking Univ, Hosp 3, Dept Ophthalmol, Beijing 100871, Peoples R China

Recommended Citation:
Lu, Qiang,Ma, Yan,Xu, Yong-sheng,et al. Apelin in epiretinal membranes of patients with proliferative diabetic retinopathy[J]. MOLECULAR VISION,2014,20:1122-1131.
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