IR@PKUHSC  > 北京大学基础医学院  > 免疫学系
学科主题基础医学
Nonspecific esterase released from thymic macrophages accumulates in the apoptotic thymocytes: an indication for this enzyme participating in the clearance of apoptotic thymocytes
Feng, JM; Wu, JS; Campagnoni, AT; Chen, WF
关键词nonspecific esterase macrophage thymocyte apoptosis
刊名EUROPEAN JOURNAL OF IMMUNOLOGY
2002-05-01
32期:5页:1386-1392
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Immunology
研究领域[WOS]Immunology
关键词[WOS]CELLS
英文摘要

In the mouse thymus, a large number of developing thymocytes die through apoptosis each day. It has been proposed that thymic macrophages are responsible for clearance of the massive number of thymocytes that die through apoptosis. The detailed clearance mechanism by which macrophages remove the apoptotic cells is not clear Our in vitro studies in this report show that nonspecific esterase (NSE), a cytochemical marker enzyme of macrophages, was secreted from thymic macrophages as a consequence of stimulation by interaction with thymocytes, and the esterase accumulated in these macrophage-binding thymocytes (MBT). TUNEL staining demonstrated that these MBT were undergoing apoptosis. The inability to exclude eosin Y and the presence of pores on the plasma membrane were further evidence for the disintegration of these MBT. In vivo, the release of NSE was evident by the presence of NSE activity in the extracellular space between the macropahges and apoptotic thymocytes under the transmission electron microscope after dexamethasone injection, which causes massive apoptosis of thymocytes. Inhibition study showed that the inhibition of NSE delayed the MBT progressing to the late apoptotic phase. These results suggest that the NSE released from macrophages is involved in the clearance of apoptotic thymocytes.

语种英语
WOS记录号WOS:000175695700020
Citation statistics
Cited Times:7[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/60967
Collection北京大学基础医学院_免疫学系
作者单位1.Univ Calif Los Angeles, Sch Med, Inst Neuropsychiat, Dev Biol Grp, Los Angeles, CA 90024 USA
2.Peking Univ, Hlth Sci Ctr, Dept Histol & Embryol, Beijing 100871, Peoples R China
3.Peking Univ, Hlth Sci Ctr, Dept Immunol, Beijing 100871, Peoples R China
Recommended Citation
GB/T 7714
Feng, JM,Wu, JS,Campagnoni, AT,et al. Nonspecific esterase released from thymic macrophages accumulates in the apoptotic thymocytes: an indication for this enzyme participating in the clearance of apoptotic thymocytes[J]. EUROPEAN JOURNAL OF IMMUNOLOGY,2002,32(5):1386-1392.
APA Feng, JM,Wu, JS,Campagnoni, AT,&Chen, WF.(2002).Nonspecific esterase released from thymic macrophages accumulates in the apoptotic thymocytes: an indication for this enzyme participating in the clearance of apoptotic thymocytes.EUROPEAN JOURNAL OF IMMUNOLOGY,32(5),1386-1392.
MLA Feng, JM,et al."Nonspecific esterase released from thymic macrophages accumulates in the apoptotic thymocytes: an indication for this enzyme participating in the clearance of apoptotic thymocytes".EUROPEAN JOURNAL OF IMMUNOLOGY 32.5(2002):1386-1392.
Files in This Item: Download All
File Name/Size DocType Version Access License
Feng_et_al-2002-Euro(254KB)期刊论文出版稿开放获取CC BY-NC-SAView Download
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
谷歌学术
谷歌学术Similar articles in
[Feng, JM]'s Articles
[Wu, JS]'s Articles
[Campagnoni, AT]'s Articles
百度学术
百度学术Similar articles in
[Feng, JM]'s Articles
[Wu, JS]'s Articles
[Campagnoni, AT]'s Articles
必应学术
必应学术Similar articles in
[Feng, JM]'s Articles
[Wu, JS]'s Articles
[Campagnoni, AT]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Feng_et_al-2002-European_Journal_of_Immunology.pdf
Format: Adobe PDF
This file does not support browsing at this time
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.