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学科主题: 临床医学
题名:
Basic fibroblast growth factor promotes macaque follicle development in vitro
作者: Lu, C. L.1,2,3; Yan, J.1,2,3; Zhi, X.1,2,3; Xia, X.4; Wang, T. R.1,2,3; Yan, L. Y.1,2,3; Yu, Y.1,2,3; Ding, T.1,2,3; Gao, J. M.1,2,3; Li, R.1,2,3; Qiao, J.1,2,3
刊名: REPRODUCTION
发表日期: 2015-05-01
DOI: 10.1530/REP-14-0557
卷: 149, 期:5, 页:425-433
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Developmental Biology ; Reproductive Biology
研究领域[WOS]: Developmental Biology ; Reproductive Biology
关键词[WOS]: HUMAN OVARIAN-FOLLICLES ; CAPRINE PREANTRAL FOLLICLES ; PRE-ANTRAL FOLLICLES ; STEADY-STATE LEVEL ; PRIMORDIAL FOLLICLES ; KIT-LIGAND ; STIMULATING-HORMONE ; MESSENGER-RNA ; INITIATES FOLLICULOGENESIS ; CANCER-PATIENTS
英文摘要:

Fertility preservation is an important type of frontier scientific research in the field of reproductive health. The culture of ovarian cortices to i) initiate primordial follicle growth and ii) procure developing follicles for later oocyte maturation is a promising fertility preservation strategy, especially for older women or cancer patients. At present, this goal remains largely unsubstantiated in primates because of the difficulty in attaining relatively large follicles via ovarian cortex culture. To overcome this hurdle, we cultured macaque monkey ovarian cortices with FSH, kit ligand (KL), basic fibroblast growth factor (bFGF), and/or epidermal growth factor (EGF). The various factors and factor combinations promoted primordial follicle development to different extents. Notably, both bFF (bFGF, 100 ng/ml and FSH, 50 ng/ml) and KF (KL, 100 ng/ml and FSH, 50 ng/ml) contributed to the activation of primordial follicles at day 12 (D12) of culture, whereas at D18, the proportions of developing follicles were significantly higher in the bFF and KF groups relative to the other treatment groups, particularly in the bFF group. Estradiol and progesterone production were also highest in the bFF group, and primary follicle diameters were the largest. Up until D24, the bFF group still exhibited the highest proportion of developing follicles. In conclusion, the bFGF-FSH combination promotes nonhuman primate primordial follicle development in vitro, with the optimal experimental window within 18 days. These results provide evidence for the future success of human ovarian cortex culture and the eventual acquisition of mature human follicles or oocytes for fertility restoration.

语种: 英语
所属项目编号: 81300456 ; 81200470 ; 31429004 ; 2011CB944504 ; 2011CB944503
项目资助者: National Natural Science Foundation of China ; National Basic Research Program of China
WOS记录号: WOS:000353660700007
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/60976
Appears in Collections:北京大学第三临床医学院_期刊论文

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作者单位: 1.Minist Educ, Key Lab Assisted Reprod, Beijing, Peoples R China
2.Peking Univ, Hosp 3, Med Ctr Human Reprod, Dept Obstet & Gynecol, Beijing 100191, Peoples R China
3.Beijing Key Lab Reprod Endocrinol & Assisted Repr, Beijing, Peoples R China
4.Peking Univ, Shenzhen Hosp, Ctr Reprod Med, Shenzhen 518000, Guangdong, Peoples R China

Recommended Citation:
Lu, C. L.,Yan, J.,Zhi, X.,et al. Basic fibroblast growth factor promotes macaque follicle development in vitro[J]. REPRODUCTION,2015,149(5):425-433.
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