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学科主题临床医学
Basic fibroblast growth factor promotes macaque follicle development in vitro
Lu, C. L.1,2,3; Yan, J.1,2,3; Zhi, X.1,2,3; Xia, X.4; Wang, T. R.1,2,3; Yan, L. Y.1,2,3; Yu, Y.1,2,3; Ding, T.1,2,3; Gao, J. M.1,2,3; Li, R.1,2,3; Qiao, J.1,2,3
刊名REPRODUCTION
2015-05-01
DOI10.1530/REP-14-0557
149期:5页:425-433
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Developmental Biology ; Reproductive Biology
研究领域[WOS]Developmental Biology ; Reproductive Biology
关键词[WOS]HUMAN OVARIAN-FOLLICLES ; CAPRINE PREANTRAL FOLLICLES ; PRE-ANTRAL FOLLICLES ; STEADY-STATE LEVEL ; PRIMORDIAL FOLLICLES ; KIT-LIGAND ; STIMULATING-HORMONE ; MESSENGER-RNA ; INITIATES FOLLICULOGENESIS ; CANCER-PATIENTS
英文摘要

Fertility preservation is an important type of frontier scientific research in the field of reproductive health. The culture of ovarian cortices to i) initiate primordial follicle growth and ii) procure developing follicles for later oocyte maturation is a promising fertility preservation strategy, especially for older women or cancer patients. At present, this goal remains largely unsubstantiated in primates because of the difficulty in attaining relatively large follicles via ovarian cortex culture. To overcome this hurdle, we cultured macaque monkey ovarian cortices with FSH, kit ligand (KL), basic fibroblast growth factor (bFGF), and/or epidermal growth factor (EGF). The various factors and factor combinations promoted primordial follicle development to different extents. Notably, both bFF (bFGF, 100 ng/ml and FSH, 50 ng/ml) and KF (KL, 100 ng/ml and FSH, 50 ng/ml) contributed to the activation of primordial follicles at day 12 (D12) of culture, whereas at D18, the proportions of developing follicles were significantly higher in the bFF and KF groups relative to the other treatment groups, particularly in the bFF group. Estradiol and progesterone production were also highest in the bFF group, and primary follicle diameters were the largest. Up until D24, the bFF group still exhibited the highest proportion of developing follicles. In conclusion, the bFGF-FSH combination promotes nonhuman primate primordial follicle development in vitro, with the optimal experimental window within 18 days. These results provide evidence for the future success of human ovarian cortex culture and the eventual acquisition of mature human follicles or oocytes for fertility restoration.

语种英语
WOS记录号WOS:000353660700007
引用统计
被引频次:2[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/60976
专题北京大学第三临床医学院
北京大学第二临床医学院_放射科
北京大学第三临床医学院_生殖医学中心
作者单位1.Minist Educ, Key Lab Assisted Reprod, Beijing, Peoples R China
2.Peking Univ, Hosp 3, Med Ctr Human Reprod, Dept Obstet & Gynecol, Beijing 100191, Peoples R China
3.Beijing Key Lab Reprod Endocrinol & Assisted Repr, Beijing, Peoples R China
4.Peking Univ, Shenzhen Hosp, Ctr Reprod Med, Shenzhen 518000, Guangdong, Peoples R China
推荐引用方式
GB/T 7714
Lu, C. L.,Yan, J.,Zhi, X.,et al. Basic fibroblast growth factor promotes macaque follicle development in vitro[J]. REPRODUCTION,2015,149(5):425-433.
APA Lu, C. L..,Yan, J..,Zhi, X..,Xia, X..,Wang, T. R..,...&Qiao, J..(2015).Basic fibroblast growth factor promotes macaque follicle development in vitro.REPRODUCTION,149(5),425-433.
MLA Lu, C. L.,et al."Basic fibroblast growth factor promotes macaque follicle development in vitro".REPRODUCTION 149.5(2015):425-433.
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