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学科主题: 口腔医学
题名:
Activity of 25-Hydroxylase in Human Gingival Fibroblasts and Periodontal Ligament Cells
作者: Liu, Kaining; Meng, Huanxin; Hou, Jianxia
刊名: PLOS ONE
发表日期: 2012-12-12
DOI: 10.1371/journal.pone.0052053
卷: 7, 期:12
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Multidisciplinary Sciences
研究领域[WOS]: Science & Technology - Other Topics
关键词[WOS]: VITAMIN-D 25-HYDROXYLASE ; TOLL-LIKE RECEPTORS ; STEROL 27-HYDROXYLASE ; HUMAN-SKIN ; 1-ALPHA,25-DIHYDROXYVITAMIN D-3 ; CEREBROTENDINOUS XANTHOMATOSIS ; AGGRESSIVE PERIODONTITIS ; CYTOCHROME P450SCC ; HUMAN MACROPHAGES ; PROSTATE CELLS
英文摘要:

Background: We previously demonstrated that 25-hydroxyvitamin D-3 concentrations in gingival crevicular fluid are 300 times higher than those in the plasma of patients with aggressive periodontitis. Here we explored whether 25-hydroxyvitamin D-3 can be synthesized by periodontal soft tissue cells. We also investigated which of the two main kinds of hydroxylases, CYP27A1 and CYP2R1, is the key 25-hydroxylase in periodontal soft tissue cells.

Methodology/Principal Findings: Primary cultures of human gingival fibroblasts and periodontal ligament cells from 5 individual donors were established. CYP27A1 mRNA, CYP2R1 mRNA and CYP27A1 protein were detected in human gingival fibroblasts and periodontal ligament cells, whereas CYP2R1 protein was not. After incubation with the 25-hydroxylase substrate vitamin D-3, human gingival fibroblasts and periodontal ligament cells generated detectable 25-hydroxyvitamin D-3 that resulted in the production of 1 alpha,25-dihydroxyvitamin D-3. Specific knockdown of CYP27A1 in human gingival fibroblasts and periodontal ligament cells using siRNA resulted in a significant reduction in both 25-hydroxyvitamin D-3 and 1 alpha,25-dihydroxyvitamin D-3 production. Knockdown of CYP2R1 did not significantly influence 25-hydroxyvitamin D-3 synthesis. Sodium butyrate did not influence significantly CYP27A1 mRNA expression; however, interleukin-1 beta and Porphyromonas gingivalis lipopolysaccharide strongly induced CYP27A1 mRNA expression in human gingival fibroblasts and periodontal ligament cells.

Conclusions: The activity of 25-hydroxylase was verified in human gingival fibroblasts and periodontal ligament cells, and CYP27A1 was identified as the key 25-hydroxylase in these cells.

语种: 英语
所属项目编号: 30772420 ; 81100749 ; 7102164
项目资助者: National Natural Science Foundations of China ; Beijing Natural Science Foundation
WOS记录号: WOS:000313236200174
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/61023
Appears in Collections:北京大学口腔医学院_牙周科_期刊论文

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作者单位: Peking Univ, Sch & Hosp Stomatol, Dept Periodontol, Beijing 100871, Peoples R China

Recommended Citation:
Liu, Kaining,Meng, Huanxin,Hou, Jianxia. Activity of 25-Hydroxylase in Human Gingival Fibroblasts and Periodontal Ligament Cells[J]. PLOS ONE,2012,7(12).
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