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学科主题: 临床医学
题名:
Dynamic changes of hepatitis B virus polymerase gene including YMDD motif in lamivudine-treated patients with chronic hepatitis B
作者: Feng, Bo1; Wei, Lai1; Chen, Ming2; Wang, Liping2
关键词: hepatitis B virus ; polymerase gene ; YMDD mutation
刊名: MICROBIOLOGICAL RESEARCH
发表日期: 2008
DOI: 10.1016/j.micres.2006.11.004
卷: 163, 期:4, 页:487-492
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Microbiology
研究领域[WOS]: Microbiology
关键词[WOS]: THERAPY ; MUTATIONS
英文摘要:

The mutation of YMDD motif of hepatitis B virus (HBV) polymerase gene is the most frequent cause in HBV resistant to lamivudine. The aim of the study was to investigate variation features of HBV polymerase gene in chronic hepatitis B (CHB) patients before and after lamivudine treatment. From the serum samples of five CHB patients before and after 12 months of lamivudine treatment, HBV polymerase gene was amplificated and positive DNA fragments were cloned into JM105 competent cell. Twenty positive clones of every sample were checked with mismatched polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and YMDD variants were sequenced. Among five patients after 12 months of lamivudine treatment, M552I mutations in two patients with HBV DNA rebounding and D553G mutation in one non-responder were detected except two patients with negative HBV DNA consecutively. In summary, D553G mutation is probably one of the reasons that caused non-responders during lamivudine treatment. The mutations of YMDD motif occurred after lamivudine treatment are caused by the induction of lamivudine. (c) 2007 Elsevier GmbH. All rights reserved.

语种: 英语
所属项目编号: 2005CB522900 ; 30571637
项目资助者: National Basic Research Program of China ; National Natural Science Foundation
WOS记录号: WOS:000258388900015
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/61029
Appears in Collections:北京大学第二临床医学院_北京大学肝病研究所_期刊论文

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作者单位: 1.Peking Univ, Peoples Hosp, Inst Hepatol, Beijing 100871, Peoples R China
2.Xuzhou Med Coll, Affiliated Hosp, Dept Infect Dis, Xuzhou, Peoples R China

Recommended Citation:
Feng, Bo,Wei, Lai,Chen, Ming,et al. Dynamic changes of hepatitis B virus polymerase gene including YMDD motif in lamivudine-treated patients with chronic hepatitis B[J]. MICROBIOLOGICAL RESEARCH,2008,163(4):487-492.
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