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学科主题: 临床医学
题名:
Up-regulation of visfatin expression in subjects with hyperthyroidism and hypothyroidism is partially relevant to a nonlinear regulation mechanism between visfatin and tri-iodothyronine with various concentrations
作者: Han Jing1; Zhang Tian-ou1; Xiao Wen-hua2; Chang Cui-qing1; Ai Hua1
关键词: thyroid dysfunction ; adipocytokines ; thyroid hormone ; 3T3-L1 cells
刊名: CHINESE MEDICAL JOURNAL
发表日期: 2012-03-05
DOI: 10.3760/cma.j.issn.0366-6999.2012.05.026
卷: 125, 期:5, 页:874-881
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Medicine, General & Internal
研究领域[WOS]: General & Internal Medicine
关键词[WOS]: COLONY-ENHANCING FACTOR ; TYPE-2 DIABETES-MELLITUS ; THYROID-FUNCTION ; SERUM-LEVELS ; INSULIN SENSITIVITY ; 3T3-L1 ADIPOCYTES ; PLASMA VISFATIN ; GRAVES-DISEASE ; VISCERAL FAT ; GENE
英文摘要:

Background Visfatin, a visceral fat-derived adipocytokine, plays a significant physiological function in lipid metabolism. However, the precise function of visfatin and its regulation by thyroid hormones are still unknown. This study observed the plasma visfatin concentrations in subjects with hyperthyroidism and hypothyroidism in vivo, and investigated the possible regulation mechanism between visfatin and tri-iodothyronine (T-3) in vitro as a further interpretation.

Methods The experiment in vivo included clinical subjects (57 patients with thyroid dysfunction and 29 euthyroid healthy volunteers) and an animal model (24 Wistar rats). All subjects were divided into hyperthyroidism, hypothyroidism and euthyroidism groups, with plasma thyroid hormones, thyrotropin, visfatin and triglyceride concentrations determined. Visfatin mRNA expression in visceral fat and liver of rats was detected by quantitative real-time reverse transcription-polymerase chain reaction (RT-PCR). The experiment in vitro studied 3T3-L1 cells and visfatin mRNA expression under nine different T-3 concentrations (0, 0.1, 0.25, 0.5, 1, 5, 10, 20, 100 nmol/L) using quantitative real-time RT-PCR.

Results Clinical subjects and animal models showed elevated plasma visfatin concentrations in the hyperthyroidism group (20.466 ng/ml (15.263, 26.795 ng/ml) and (1209.164+/-165.292) ng/L) and hypothyroidism group (12.457 ng/ml (11.115, 15.454 ng/ml) and (1205.425+/-109.200) ng/L) compared to euthyroidism group (6.891 ng/ml (5.888, 8.803 ng/ml) and (926.650+/-54.002) ng/L, P <0.001). For animal models, visfatin mRNA expression in visceral fat in the hyperthyroidism and hypothyroidism groups increased about 3.33-fold and 1.98-fold compared to the euthyroidism group (P <0.001), which was positively correlated with plasma visfatin concentrations (r=0.713, P <0.001). However, no significant group difference (P >0.05) and correlation (r=0.121, P=0.572) was found in the liver. T-3 induced a remarkable increase of visfatin mRNA expression in 3T3-L1 cells at low concentrations (0-0.5 nmol/L T-3) followed by a sharp decrease at higher concentrations (0.5-100 nmol/L T-3), with an inflection point at 0.5 nmol/L T-3.

Conclusion Elevated circulating visfatin levels in subjects with hyperthyroidism and hypothyroidism are possibly due to an increase of visfatin mRNA expression in visceral fat, and a nonlinear regulation mechanism on visfatin mRNA expression under various T-3 concentrations might be involved. Chin Med J 2012;125(5):874-881

语种: 英语
所属项目编号: 30270636 ; 30671015
项目资助者: National Natural Science Foundation of China
WOS记录号: WOS:000301755200028
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/61074
Appears in Collections:北京大学第三临床医学院_运动医学研究所_期刊论文

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作者单位: 1.Peking Univ, Hosp 3, Dept Biochem & Nutr, Inst Sports Med, Beijing 100191, Peoples R China
2.Peking Univ, Hosp 3, Dept Endocrinol, Beijing 100191, Peoples R China

Recommended Citation:
Han Jing,Zhang Tian-ou,Xiao Wen-hua,et al. Up-regulation of visfatin expression in subjects with hyperthyroidism and hypothyroidism is partially relevant to a nonlinear regulation mechanism between visfatin and tri-iodothyronine with various concentrations[J]. CHINESE MEDICAL JOURNAL,2012,125(5):874-881.
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