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学科主题: 临床医学
题名:
A novel combination of immunoreaction and ICP-MS as a hyphenated technique for the determination of thyroid-stimulating hormone (TSH) in human serum
作者: Zhang, C; Wu, FB; Zhang, YY; Wang, X; Zhang, XR
刊名: JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY
发表日期: 2001
DOI: 10.1039/b106387c
卷: 16, 期:12, 页:1393-1396
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Chemistry, Analytical ; Spectroscopy
研究领域[WOS]: Chemistry ; Spectroscopy
关键词[WOS]: PLASMA-MASS SPECTROMETRY ; DYNAMIC REACTION CELL ; COLLOIDAL GOLD ; IMMUNOASSAYS ; ELECTROPHORESIS ; PERFORMANCE
英文摘要:

The novel coupling method based on the hyphenation of immunoreaction with ICP-MS was developed and applied to the determination of thyroid-stimulating hormone (TSH) in human serum. In this system, TSH was firstly captured by anti-TSH monoclonal antibodies immobilized on a solid support. Biotinylated anti-TSH monoclonal antibodies and Eu3+-labelled streptavidin were then added to form the complex, captured antibody-TSH-biotinylated anti-TSH antibody-Eu3+-labelled streptavidin. After unbound reactants were washed away, Eu3+ bound to the complex was extracted by 1% HNO3 and measured by ICP-MS. The Eu signal was directly proportional to the serum TSH concentration. The limit of detection was 0.5 mIU L-1 and the assay was linear up to 170 mIU L-1. The within-run (n = 6) and between-run (n = 6) relative standard deviations (RSD) for low, medium and high concentration standards did not exceed 10%. The TSH results obtained by this procedure correlated well with those obtained by radioimmunoassay (r(2) = 0.9639).

语种: 英语
WOS记录号: WOS:000172516100008
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/61089
Appears in Collections:北京大学第三临床医学院_期刊论文

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作者单位: 1.Tsing Hua Univ, Dept Chem, Beijing 100084, Peoples R China
2.Peking Univ, Hosp 3, Beijing 100083, Peoples R China
3.Inst Atom Energy, Dept Isotope, Lab Immunoassay, Beijing 102413, Peoples R China

Recommended Citation:
Zhang, C,Wu, FB,Zhang, YY,et al. A novel combination of immunoreaction and ICP-MS as a hyphenated technique for the determination of thyroid-stimulating hormone (TSH) in human serum[J]. JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY,2001,16(12):1393-1396.
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