北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学第三临床医学院  > 期刊论文
学科主题: 临床医学
题名:
A novel combination of immunoreaction and ICP-MS as a hyphenated technique for the determination of thyroid-stimulating hormone (TSH) in human serum
作者: Zhang, C; Wu, FB; Zhang, YY; Wang, X; Zhang, XR
刊名: JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY
发表日期: 2001
DOI: 10.1039/b106387c
卷: 16, 期:12, 页:1393-1396
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Chemistry, Analytical ; Spectroscopy
研究领域[WOS]: Chemistry ; Spectroscopy
关键词[WOS]: PLASMA-MASS SPECTROMETRY ; DYNAMIC REACTION CELL ; COLLOIDAL GOLD ; IMMUNOASSAYS ; ELECTROPHORESIS ; PERFORMANCE
英文摘要:

The novel coupling method based on the hyphenation of immunoreaction with ICP-MS was developed and applied to the determination of thyroid-stimulating hormone (TSH) in human serum. In this system, TSH was firstly captured by anti-TSH monoclonal antibodies immobilized on a solid support. Biotinylated anti-TSH monoclonal antibodies and Eu3+-labelled streptavidin were then added to form the complex, captured antibody-TSH-biotinylated anti-TSH antibody-Eu3+-labelled streptavidin. After unbound reactants were washed away, Eu3+ bound to the complex was extracted by 1% HNO3 and measured by ICP-MS. The Eu signal was directly proportional to the serum TSH concentration. The limit of detection was 0.5 mIU L-1 and the assay was linear up to 170 mIU L-1. The within-run (n = 6) and between-run (n = 6) relative standard deviations (RSD) for low, medium and high concentration standards did not exceed 10%. The TSH results obtained by this procedure correlated well with those obtained by radioimmunoassay (r(2) = 0.9639).

语种: 英语
WOS记录号: WOS:000172516100008
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/61089
Appears in Collections:北京大学第三临床医学院_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Tsing Hua Univ, Dept Chem, Beijing 100084, Peoples R China
2.Peking Univ, Hosp 3, Beijing 100083, Peoples R China
3.Inst Atom Energy, Dept Isotope, Lab Immunoassay, Beijing 102413, Peoples R China

Recommended Citation:
Zhang, C,Wu, FB,Zhang, YY,et al. A novel combination of immunoreaction and ICP-MS as a hyphenated technique for the determination of thyroid-stimulating hormone (TSH) in human serum[J]. JOURNAL OF ANALYTICAL ATOMIC SPECTROMETRY,2001,16(12):1393-1396.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Zhang, C]'s Articles
[Wu, FB]'s Articles
[Zhang, YY]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Zhang, C]‘s Articles
[Wu, FB]‘s Articles
[Zhang, YY]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace