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An approach to identifying sequential metabolites of a typical phenylethanoid glycoside, echinacoside, based on liquid chromatography-ion trap-time of flight mass spectrometry analysis
Wang, Yuxin1; Hao, Haiping1; Wang, Guangji1; Tu, Pengfei2; Jiang, Yong2; Liang, Yan1; Dai, Liang1; Yang, Hao1; Lai, Li1; Zheng, Chaonan1; Wang, Qiong1; Cui, Nan1; Liu, Yitong1
关键词Phenylethanoid glycoside Echinacoside LC/MS-IT-TOF Metabolite identification
刊名TALANTA
2009-12-15
DOI10.1016/j.talanta.2009.07.027
80期:2页:572-580
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Chemistry, Analytical
研究领域[WOS]Chemistry
关键词[WOS]TRANSFORMATION PRODUCTS ; CISTANCHE DESERTICOLA ; ANTIOXIDANT ACTIVITY ; ROOT EXTRACT ; IDENTIFICATION ; PESTICIDES ; SAMPLES ; FOOD ; COMPONENTS ; STRATEGY
英文摘要

Metabolite identification for the compounds that undergo multiple and sequential metabolism is still a great challenge. Echinacoside (ECH), a typical phenylethanoid glycoside, contains multiple unstable chemical bonds and high reactive functional groups which are susceptible to multiple pathways of degradation and metabolism, leading great difficulties for its metabolite identification. This study proposed a novel approach for rapidly identifying the complicated and unpredictable metabolites of ECH, based on the powerful liquid chromatography hybrid ion trap and time of flight mass spectrometry (LC/MS-IT-TOF) analysis. Four degradation products were rapidly identified via the "fragmentation-degradation" comparisons. Five phase I and phase II metabolites of the degradation products were rapidly characterized via the crossover mass differences comparisons of their quasi-molecular ions with the potential precursors. Four direct phase I and phase II metabolites of the parent compound were identified by the mass differences analysis of the molecular ions between metabolites and the parent compound. Multiple stages of fragmentation patterns were used to confirm the metabolites characterizations. This study provides a novel approach to characterizing the complicated metabolites, and would be widely applicable for the metabolite identification of natural products. (C) 2009 Elsevier B.V. All rights reserved.

语种英语
WOS记录号WOS:000271701900026
项目编号30630076 ; 2006BA1081304-05
资助机构National Natural Science Foundation of China ; Ministry of Science and Technology of PR China
引用统计
被引频次:45[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/61094
专题北京大学药学院_天然药物学系
作者单位1.China Pharmaceut Univ, Key Lab Drug Metab & Pharmacokinet, Nanjing 210009, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Sch Pharmaceut Sci, Dept Nat Med, Beijing 100083, Peoples R China
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Wang, Yuxin,Hao, Haiping,Wang, Guangji,et al. An approach to identifying sequential metabolites of a typical phenylethanoid glycoside, echinacoside, based on liquid chromatography-ion trap-time of flight mass spectrometry analysis[J]. TALANTA,2009,80(2):572-580.
APA Wang, Yuxin.,Hao, Haiping.,Wang, Guangji.,Tu, Pengfei.,Jiang, Yong.,...&Liu, Yitong.(2009).An approach to identifying sequential metabolites of a typical phenylethanoid glycoside, echinacoside, based on liquid chromatography-ion trap-time of flight mass spectrometry analysis.TALANTA,80(2),572-580.
MLA Wang, Yuxin,et al."An approach to identifying sequential metabolites of a typical phenylethanoid glycoside, echinacoside, based on liquid chromatography-ion trap-time of flight mass spectrometry analysis".TALANTA 80.2(2009):572-580.
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