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学科主题: 药学
题名:
An approach to identifying sequential metabolites of a typical phenylethanoid glycoside, echinacoside, based on liquid chromatography-ion trap-time of flight mass spectrometry analysis
作者: Wang, Yuxin1; Hao, Haiping1; Wang, Guangji1; Tu, Pengfei2; Jiang, Yong2; Liang, Yan1; Dai, Liang1; Yang, Hao1; Lai, Li1; Zheng, Chaonan1; Wang, Qiong1; Cui, Nan1; Liu, Yitong1
关键词: Phenylethanoid glycoside ; Echinacoside ; LC/MS-IT-TOF ; Metabolite identification
刊名: TALANTA
发表日期: 2009-12-15
DOI: 10.1016/j.talanta.2009.07.027
卷: 80, 期:2, 页:572-580
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Chemistry, Analytical
研究领域[WOS]: Chemistry
关键词[WOS]: TRANSFORMATION PRODUCTS ; CISTANCHE DESERTICOLA ; ANTIOXIDANT ACTIVITY ; ROOT EXTRACT ; IDENTIFICATION ; PESTICIDES ; SAMPLES ; FOOD ; COMPONENTS ; STRATEGY
英文摘要:

Metabolite identification for the compounds that undergo multiple and sequential metabolism is still a great challenge. Echinacoside (ECH), a typical phenylethanoid glycoside, contains multiple unstable chemical bonds and high reactive functional groups which are susceptible to multiple pathways of degradation and metabolism, leading great difficulties for its metabolite identification. This study proposed a novel approach for rapidly identifying the complicated and unpredictable metabolites of ECH, based on the powerful liquid chromatography hybrid ion trap and time of flight mass spectrometry (LC/MS-IT-TOF) analysis. Four degradation products were rapidly identified via the "fragmentation-degradation" comparisons. Five phase I and phase II metabolites of the degradation products were rapidly characterized via the crossover mass differences comparisons of their quasi-molecular ions with the potential precursors. Four direct phase I and phase II metabolites of the parent compound were identified by the mass differences analysis of the molecular ions between metabolites and the parent compound. Multiple stages of fragmentation patterns were used to confirm the metabolites characterizations. This study provides a novel approach to characterizing the complicated metabolites, and would be widely applicable for the metabolite identification of natural products. (C) 2009 Elsevier B.V. All rights reserved.

语种: 英语
所属项目编号: 30630076 ; 2006BA1081304-05
项目资助者: National Natural Science Foundation of China ; Ministry of Science and Technology of PR China
WOS记录号: WOS:000271701900026
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/61094
Appears in Collections:北京大学药学院_天然药物学系_期刊论文

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作者单位: 1.China Pharmaceut Univ, Key Lab Drug Metab & Pharmacokinet, Nanjing 210009, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Sch Pharmaceut Sci, Dept Nat Med, Beijing 100083, Peoples R China

Recommended Citation:
Wang, Yuxin,Hao, Haiping,Wang, Guangji,et al. An approach to identifying sequential metabolites of a typical phenylethanoid glycoside, echinacoside, based on liquid chromatography-ion trap-time of flight mass spectrometry analysis[J]. TALANTA,2009,80(2):572-580.
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