IR@PKUHSC  > 北京大学药学院
学科主题药学
Metal binding discrimination of the calmodulin Q41C/K75C mutant on Ca2+ and La3+
Xu Kun; Yang XiaoDa; Wang Kui
关键词calmodulin lanthanum calcium long range interaction
刊名SCIENCE CHINA-CHEMISTRY
2010-04-01
DOI10.1007/s11426-010-0059-2
53期:4页:797-806
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Chemistry, Multidisciplinary
研究领域[WOS]Chemistry
关键词[WOS]DROSOPHILA-MELANOGASTER CALMODULIN ; CALCIUM-BINDING ; SITE MUTANTS ; PROTEINS ; AFFINITY ; DIVERSITY ; PEPTIDE ; SERIES
英文摘要

Calmodulin (CaM) is a multifunctional Ca2+-binding protein regulating the activity of many enzymes in response to fluctuation of the intracellular Ca2+ level. It has been shown that a CaM Q41C/K75C mutant (CaMSS) with a disulfide bond in the N-terminal domain exhibits greatly reduced affinity to Ca2+. In the present study, the experimental results revealed a unique metal binding pattern in CaMSS towards La3+ and Ca2+ separately: the mutant protein binds Ca2+ at site I, III and IV; however, it binds La3+ at site I, II and IV. A putative mechanism was proposed which is the conformation of site II (or site III) of CaMSS could be altered and thus loses its metal ion affinity in response to metal binding in the opposite terminal domain possibly through the long range domain interaction. The present work may offer new perspectives for understanding the mechanisms of specific metal ion affinity in CaM and for CaM-based protein design.

语种英语
WOS记录号WOS:000278263400013
项目编号20671008 ; 20637010
资助机构National Natural Science Foundation of China ; Key Construction Program of the National "985" Project
引用统计
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/61095
专题北京大学药学院
北京大学药学院_化学生物学系
北京大学医学图书馆_流通阅览部
作者单位Peking Univ, Hlth Sci Ctr, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs,Dept Biol Chem, Beijing 100191, Peoples R China
推荐引用方式
GB/T 7714
Xu Kun,Yang XiaoDa,Wang Kui. Metal binding discrimination of the calmodulin Q41C/K75C mutant on Ca2+ and La3+[J]. SCIENCE CHINA-CHEMISTRY,2010,53(4):797-806.
APA Xu Kun,Yang XiaoDa,&Wang Kui.(2010).Metal binding discrimination of the calmodulin Q41C/K75C mutant on Ca2+ and La3+.SCIENCE CHINA-CHEMISTRY,53(4),797-806.
MLA Xu Kun,et al."Metal binding discrimination of the calmodulin Q41C/K75C mutant on Ca2+ and La3+".SCIENCE CHINA-CHEMISTRY 53.4(2010):797-806.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Xu Kun]的文章
[Yang XiaoDa]的文章
[Wang Kui]的文章
百度学术
百度学术中相似的文章
[Xu Kun]的文章
[Yang XiaoDa]的文章
[Wang Kui]的文章
必应学术
必应学术中相似的文章
[Xu Kun]的文章
[Yang XiaoDa]的文章
[Wang Kui]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。