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学科主题: 基础医学
题名:
AN ANG1-TIE2-PI3K AXIS IN NEURAL PROGENITOR CELLS INITIATES SURVIVAL RESPONSES AGAINST OXYGEN AND GLUCOSE DEPRIVATION
作者: Bai, Y.1; Meng, Z.1; Cui, M.2; Zhang, X.1; Chen, F.2; Xiao, J.1; Shen, L.1; Zhang, Y.1
关键词: angiopoietin-1 ; Akt ; nestin ; cerebral ischemia ; apoptosis ; stem cell
刊名: NEUROSCIENCE
发表日期: 2009-05-05
DOI: 10.1016/j.neuroscience.2009.01.076
卷: 160, 期:2, 页:371-381
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Neurosciences
研究领域[WOS]: Neurosciences & Neurology
关键词[WOS]: INTERMEDIATE-FILAMENT PROTEIN ; CNS STEM-CELLS ; SUBVENTRICULAR ZONE ; ENDOTHELIAL-CELLS ; INDUCED APOPTOSIS ; ISCHEMIC BRAIN ; IN-VITRO ; ANGIOPOIETIN-1 ; NEUROGENESIS ; EXPRESSION
英文摘要:

Neural progenitor cells (NPCs) have the potential to survive brain ischemia and participate in neurogenesis after stroke. However, it is not clear how survival responses are initiated in NPCs. Using embryonic mouse NPCs and the in vitro oxygen and glucose deprivation (OGD) model, we found that angiopoietin-1 (Ang1) could prevent NPCs from OGD-induced apoptosis, as evidenced by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling and annexin V labeling. Ang1 significantly elevated tunica intima endothelial kinase 2 (Tie2) autophosphorylation level, suggesting the existence of functional Tie2 receptors on NPCs. NPCs under OGD conditions exhibited reduction of Akt phosphorylation, decrease of the Bcl-2/Bax ratio, activation of caspase-3, cleavage of PARP, and downregulation of beta-catenin and nestin. Ang1 reversed the above changes concomitantly with significant rising of survival rates of NPCs under OGD, but all these effects of Ang1 could be blocked by either soluble extracellular domain of Tie2 Fc fusion protein (sTie2Fc) or the phosphoinositide 3-kinase (PI3K) inhibitor 2-(4-morpholinyl)-8-phenyl-1(4H)-benzopyran-4-one (LY294002). Our findings suggest the existence of an Ang1-Tie2-PI3K signaling axis that is essential in initiation of survival responses in NPCs against cerebral ischemia and hypoxia. (C) 2009 IBRO. Published by Elsevier Ltd. All rights reserved.

语种: 英语
所属项目编号: 30600167
项目资助者: National Natural Sciences Foundation of China
WOS记录号: WOS:000265426700012
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/61126
Appears in Collections:基础医学院_细胞生物学系_期刊论文

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作者单位: 1.Peking Univ, Hlth Sci Ctr, Sch Basic Med Sci, Dept Cell Biol, Beijing 100191, Peoples R China
2.Peking Univ, Hosp 3, Dept Cardiol, Beijing 100191, Peoples R China

Recommended Citation:
Bai, Y.,Meng, Z.,Cui, M.,et al. AN ANG1-TIE2-PI3K AXIS IN NEURAL PROGENITOR CELLS INITIATES SURVIVAL RESPONSES AGAINST OXYGEN AND GLUCOSE DEPRIVATION[J]. NEUROSCIENCE,2009,160(2):371-381.
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