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学科主题基础医学
AN ANG1-TIE2-PI3K AXIS IN NEURAL PROGENITOR CELLS INITIATES SURVIVAL RESPONSES AGAINST OXYGEN AND GLUCOSE DEPRIVATION
Bai, Y.1; Meng, Z.1; Cui, M.2; Zhang, X.1; Chen, F.2; Xiao, J.1; Shen, L.1; Zhang, Y.1
关键词angiopoietin-1 Akt nestin cerebral ischemia apoptosis stem cell
刊名NEUROSCIENCE
2009-05-05
DOI10.1016/j.neuroscience.2009.01.076
160期:2页:371-381
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Neurosciences
研究领域[WOS]Neurosciences & Neurology
关键词[WOS]INTERMEDIATE-FILAMENT PROTEIN ; CNS STEM-CELLS ; SUBVENTRICULAR ZONE ; ENDOTHELIAL-CELLS ; INDUCED APOPTOSIS ; ISCHEMIC BRAIN ; IN-VITRO ; ANGIOPOIETIN-1 ; NEUROGENESIS ; EXPRESSION
英文摘要

Neural progenitor cells (NPCs) have the potential to survive brain ischemia and participate in neurogenesis after stroke. However, it is not clear how survival responses are initiated in NPCs. Using embryonic mouse NPCs and the in vitro oxygen and glucose deprivation (OGD) model, we found that angiopoietin-1 (Ang1) could prevent NPCs from OGD-induced apoptosis, as evidenced by terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling and annexin V labeling. Ang1 significantly elevated tunica intima endothelial kinase 2 (Tie2) autophosphorylation level, suggesting the existence of functional Tie2 receptors on NPCs. NPCs under OGD conditions exhibited reduction of Akt phosphorylation, decrease of the Bcl-2/Bax ratio, activation of caspase-3, cleavage of PARP, and downregulation of beta-catenin and nestin. Ang1 reversed the above changes concomitantly with significant rising of survival rates of NPCs under OGD, but all these effects of Ang1 could be blocked by either soluble extracellular domain of Tie2 Fc fusion protein (sTie2Fc) or the phosphoinositide 3-kinase (PI3K) inhibitor 2-(4-morpholinyl)-8-phenyl-1(4H)-benzopyran-4-one (LY294002). Our findings suggest the existence of an Ang1-Tie2-PI3K signaling axis that is essential in initiation of survival responses in NPCs against cerebral ischemia and hypoxia. (C) 2009 IBRO. Published by Elsevier Ltd. All rights reserved.

语种英语
WOS记录号WOS:000265426700012
项目编号30600167
资助机构National Natural Sciences Foundation of China
引用统计
被引频次:21[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/61126
专题北京大学基础医学院_细胞生物学系
北京大学基础医学院
北京大学第三临床医学院_心血管内科
北京大学第三临床医学院_运动医学研究所
作者单位1.Peking Univ, Hlth Sci Ctr, Sch Basic Med Sci, Dept Cell Biol, Beijing 100191, Peoples R China
2.Peking Univ, Hosp 3, Dept Cardiol, Beijing 100191, Peoples R China
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GB/T 7714
Bai, Y.,Meng, Z.,Cui, M.,et al. AN ANG1-TIE2-PI3K AXIS IN NEURAL PROGENITOR CELLS INITIATES SURVIVAL RESPONSES AGAINST OXYGEN AND GLUCOSE DEPRIVATION[J]. NEUROSCIENCE,2009,160(2):371-381.
APA Bai, Y..,Meng, Z..,Cui, M..,Zhang, X..,Chen, F..,...&Zhang, Y..(2009).AN ANG1-TIE2-PI3K AXIS IN NEURAL PROGENITOR CELLS INITIATES SURVIVAL RESPONSES AGAINST OXYGEN AND GLUCOSE DEPRIVATION.NEUROSCIENCE,160(2),371-381.
MLA Bai, Y.,et al."AN ANG1-TIE2-PI3K AXIS IN NEURAL PROGENITOR CELLS INITIATES SURVIVAL RESPONSES AGAINST OXYGEN AND GLUCOSE DEPRIVATION".NEUROSCIENCE 160.2(2009):371-381.
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