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Antagonism of Cortex Periplocae extract-induced catecholamines secretion by Panax notoginseng saponins in cultured bovine adrenal medullary cells by drug combinations
Guo, Hao1,2,5; Mao, Haoping1,2,5; Pan, Guixiang1,2,5; Zhang, Han1,2,5; Fan, Ganwei1,2,5; Li, Weiwei3; Zhou, Kun1,2,5; Zhu, Yan1,2,5; Yanagihara, Nobuyuki4; Gao, Xiumei1,2,5
关键词Catecholamines secretion Periplocin Combinations Calcium influx
刊名JOURNAL OF ETHNOPHARMACOLOGY
2013-05-20
DOI10.1016/j.jep.2013.03.036
147期:2页:447-455
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Plant Sciences ; Chemistry, Medicinal ; Integrative & Complementary Medicine ; Pharmacology & Pharmacy
研究领域[WOS]Plant Sciences ; Pharmacology & Pharmacy ; Integrative & Complementary Medicine
关键词[WOS]CHRONIC HEART-FAILURE ; CHROMAFFIN CELLS ; VENTRICULAR-ARRHYTHMIAS ; NA/K PUMP ; OUABAIN ; RELEASE ; CALCIUM ; STRESS ; ISOPROTERENOL ; HYPERTENSION
英文摘要

Ethnopharmacological relevance: Traditional Chinese Medicine (TCM) operates on the general principle that compatible components of different herbal decoction may work together to synergistically enhance therapeutic efficacy or reduce adverse effects. Cortex Periplocae is an herb that has been used in TCM clinics for a long time in the treatment of chronic heart failure. However, recently, the use of this herb has been restricted because of widespread abuse and misapplications. Radix Notoginseng is another herb that is used in TCM because of its protective role on cardiomyocytes. From our previous studies on these two herbs in a mouse model, we observed an increased LD50 after oral administration of Cortex Periplocae extract (CPE) and Panax notoginseng saponins (PNS) in a ratio of 1:1 compared with Cortex Periplocae extract used alone.

Aim of the study: This study aimed to investigate whether there are mutual synergistic effects of the two herbal extracts, CPE and PNS, on catecholamines (CAs) secretion, and their possible underlying mechanism(s) for such effects.

Materials and methods: CPE and PNS were quantified by the LC-MS/MS method. HPLC-ECD was used to determine the CAs secreted into the medium by bovine adrenal medulla cells (BAMCs) and calcium influx was measured using a Calcium 4 reagent kit.

Results: We found that the stimulatory effect of CPE on CAs secretion was inhibited when used together with PNS. For a better clarification of the different constituents of the extracts, a quantitative analysis was carried out Periplocin was found to be the main active component of CPE valued as 0.99% and saponins were the principal constituents of PNS. These results also showed that CPE increased the secretion of CAs in a dose-dependent manner while the actions of PNS were seen to be inhibitory. Periplocin monomer of CPE could be implicated for the actions of CPE since it plays the role of increasing the ACh-induced CAs secretion in a calcium-dependent manner. We therefore conclude that; CPE and PNS exert antagonistic effects in regulating the concentration of intracellular calcium.

Conclusions: PNS inhibits CPE-induced CAs secretion by suppressing calcium influx in bovine adrenal medulla cells while periplocin, one of the main components of CPE has the same secretagogue effect as CPE. (C) 2013 Published by Elsevier Ireland Ltd.

语种英语
WOS记录号WOS:000319180700024
项目编号2012CB518400 ; 81125024 ; 2012ZX09304007
资助机构National Key Basic Research Program of China ; National Natural Science Foundation of China ; National Key Technology Research and Development Program of China
引用统计
被引频次:10[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/61139
专题北京大学第一临床医学院
作者单位1.Tianjin Univ Tradit Chinese Med, Tianjin State Key Lab Modern Chinese Med, Tianjin 300193, Peoples R China
2.Minist Educ, Tianjin Key Lab Tradit Chinese Med Pharmacol, Key Lab Pharmacol Tradit Chinese Med Formulae, Tianjin, Peoples R China
3.Peking Univ, Hosp 1, Integrated Labs Tradit Chinese Med & Western Med, Beijing 100871, Peoples R China
4.Univ Occupat & Environm Hlth, Sch Med, Dept Pharmacol, Yahatanishi Ku, Kitakyushu, Fukuoka 807, Japan
5.Tianjin Univ Tradit Chinese Med, Inst Tradit Chinese Med, Tianjin 300193, Peoples R China
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GB/T 7714
Guo, Hao,Mao, Haoping,Pan, Guixiang,et al. Antagonism of Cortex Periplocae extract-induced catecholamines secretion by Panax notoginseng saponins in cultured bovine adrenal medullary cells by drug combinations[J]. JOURNAL OF ETHNOPHARMACOLOGY,2013,147(2):447-455.
APA Guo, Hao.,Mao, Haoping.,Pan, Guixiang.,Zhang, Han.,Fan, Ganwei.,...&Gao, Xiumei.(2013).Antagonism of Cortex Periplocae extract-induced catecholamines secretion by Panax notoginseng saponins in cultured bovine adrenal medullary cells by drug combinations.JOURNAL OF ETHNOPHARMACOLOGY,147(2),447-455.
MLA Guo, Hao,et al."Antagonism of Cortex Periplocae extract-induced catecholamines secretion by Panax notoginseng saponins in cultured bovine adrenal medullary cells by drug combinations".JOURNAL OF ETHNOPHARMACOLOGY 147.2(2013):447-455.
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