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学科主题: 临床医学
题名:
Antagonism of Cortex Periplocae extract-induced catecholamines secretion by Panax notoginseng saponins in cultured bovine adrenal medullary cells by drug combinations
作者: Guo, Hao1,2,5; Mao, Haoping1,2,5; Pan, Guixiang1,2,5; Zhang, Han1,2,5; Fan, Ganwei1,2,5; Li, Weiwei3; Zhou, Kun1,2,5; Zhu, Yan1,2,5; Yanagihara, Nobuyuki4; Gao, Xiumei1,2,5
关键词: Catecholamines secretion ; Periplocin ; Combinations ; Calcium influx
刊名: JOURNAL OF ETHNOPHARMACOLOGY
发表日期: 2013-05-20
DOI: 10.1016/j.jep.2013.03.036
卷: 147, 期:2, 页:447-455
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Plant Sciences ; Chemistry, Medicinal ; Integrative & Complementary Medicine ; Pharmacology & Pharmacy
研究领域[WOS]: Plant Sciences ; Pharmacology & Pharmacy ; Integrative & Complementary Medicine
关键词[WOS]: CHRONIC HEART-FAILURE ; CHROMAFFIN CELLS ; VENTRICULAR-ARRHYTHMIAS ; NA/K PUMP ; OUABAIN ; RELEASE ; CALCIUM ; STRESS ; ISOPROTERENOL ; HYPERTENSION
英文摘要:

Ethnopharmacological relevance: Traditional Chinese Medicine (TCM) operates on the general principle that compatible components of different herbal decoction may work together to synergistically enhance therapeutic efficacy or reduce adverse effects. Cortex Periplocae is an herb that has been used in TCM clinics for a long time in the treatment of chronic heart failure. However, recently, the use of this herb has been restricted because of widespread abuse and misapplications. Radix Notoginseng is another herb that is used in TCM because of its protective role on cardiomyocytes. From our previous studies on these two herbs in a mouse model, we observed an increased LD50 after oral administration of Cortex Periplocae extract (CPE) and Panax notoginseng saponins (PNS) in a ratio of 1:1 compared with Cortex Periplocae extract used alone.

Aim of the study: This study aimed to investigate whether there are mutual synergistic effects of the two herbal extracts, CPE and PNS, on catecholamines (CAs) secretion, and their possible underlying mechanism(s) for such effects.

Materials and methods: CPE and PNS were quantified by the LC-MS/MS method. HPLC-ECD was used to determine the CAs secreted into the medium by bovine adrenal medulla cells (BAMCs) and calcium influx was measured using a Calcium 4 reagent kit.

Results: We found that the stimulatory effect of CPE on CAs secretion was inhibited when used together with PNS. For a better clarification of the different constituents of the extracts, a quantitative analysis was carried out Periplocin was found to be the main active component of CPE valued as 0.99% and saponins were the principal constituents of PNS. These results also showed that CPE increased the secretion of CAs in a dose-dependent manner while the actions of PNS were seen to be inhibitory. Periplocin monomer of CPE could be implicated for the actions of CPE since it plays the role of increasing the ACh-induced CAs secretion in a calcium-dependent manner. We therefore conclude that; CPE and PNS exert antagonistic effects in regulating the concentration of intracellular calcium.

Conclusions: PNS inhibits CPE-induced CAs secretion by suppressing calcium influx in bovine adrenal medulla cells while periplocin, one of the main components of CPE has the same secretagogue effect as CPE. (C) 2013 Published by Elsevier Ireland Ltd.

语种: 英语
所属项目编号: 2012CB518400 ; 81125024 ; 2012ZX09304007
项目资助者: National Key Basic Research Program of China ; National Natural Science Foundation of China ; National Key Technology Research and Development Program of China
WOS记录号: WOS:000319180700024
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/61139
Appears in Collections:北京大学第一临床医学院_期刊论文

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作者单位: 1.Tianjin Univ Tradit Chinese Med, Tianjin State Key Lab Modern Chinese Med, Tianjin 300193, Peoples R China
2.Minist Educ, Tianjin Key Lab Tradit Chinese Med Pharmacol, Key Lab Pharmacol Tradit Chinese Med Formulae, Tianjin, Peoples R China
3.Peking Univ, Hosp 1, Integrated Labs Tradit Chinese Med & Western Med, Beijing 100871, Peoples R China
4.Univ Occupat & Environm Hlth, Sch Med, Dept Pharmacol, Yahatanishi Ku, Kitakyushu, Fukuoka 807, Japan
5.Tianjin Univ Tradit Chinese Med, Inst Tradit Chinese Med, Tianjin 300193, Peoples R China

Recommended Citation:
Guo, Hao,Mao, Haoping,Pan, Guixiang,et al. Antagonism of Cortex Periplocae extract-induced catecholamines secretion by Panax notoginseng saponins in cultured bovine adrenal medullary cells by drug combinations[J]. JOURNAL OF ETHNOPHARMACOLOGY,2013,147(2):447-455.
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