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学科主题药学
siRNA Has Greatly Elevated Mismatch Tolerance at 3 ′-UTR Sites
Wei, Na1; Zhang, Lei1; Huang, Huang1; Chen, Yue2; Zheng, Jie1; Zhou, Xiao1; Yi, Fan1; Du, Quan1,2; Liang, Zicai1
刊名PLOS ONE
2012-11-08
DOI10.1371/journal.pone.0049309
7期:11
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Multidisciplinary Sciences
研究领域[WOS]Science & Technology - Other Topics
关键词[WOS]RNAI ENZYME COMPLEX ; DOUBLE-STRANDED-RNA ; MESSENGER-RNAS ; CAENORHABDITIS-ELEGANS ; GENE-REGULATION ; AGO PROTEINS ; TARGET RNA ; ARGONAUTE ; INTERFERENCE ; MICRORNA
英文摘要

It has been noted that target sites located in the coding region or the 3′-untranslated region (3′-UTR) can be silenced to significantly different levels by the same siRNA, but little is known about at what specificity the silencing was achieved. In an exploration of positional effects on siRNA specificity by luciferase reporter system, we surprisingly discovered that siRNA had greatly elevated tolerance towards mismatches in target sites in the 3′-UTR of the mRNA compared with the same target sites cloned in the coding region. Assessment of changes in protein and mRNA levels suggested that the differential mismatch tolerance might have resulted from location-specific translational repression in the 3′-UTR. Ablation of argonaute proteins by AGO-specific siRNAs revealed that the AGO2 had major impact on siRNA silencing activity against sites in both coding region and 3′-UTR, while the silencing of nonnucleolytic AGO proteins (AGO1, AGO3 and AGO4) did not significantly affect silencing of sites in either region. This paper revealed the discovery that the specificity of an siRNA can be affected by the location of its target site. Citation: Wei N, Zhang L, Huang H, Chen Y, Zheng J, et al. (2012) siRNA Has Greatly Elevated Mismatch Tolerance at 3′-UTR Sites. PLoS ONE 7(11): e49309. doi:10.1371/journal.pone.0049309

语种英语
WOS记录号WOS:000312269500101
项目编号30871385 ; 30873187 ; 5092011 ; 2011CBA01102 ; 10411969100 ; 20090001110052 ; 200800010019
资助机构National Natural Science Foundation of China ; Beijing Natural Science Foundation ; National Basic Research Program of China ; Shanghai Science and Technology Committee ; Department of Education of China
引用统计
被引频次:7[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/61149
专题北京大学医学图书馆_流通阅览部
北京大学医学部管理机构_后勤处
北京大学药学院
作者单位1.Peking Univ, Inst Mol Med, Beijing 100871, Peoples R China
2.Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100871, Peoples R China
推荐引用方式
GB/T 7714
Wei, Na,Zhang, Lei,Huang, Huang,et al. siRNA Has Greatly Elevated Mismatch Tolerance at 3 ′-UTR Sites[J]. PLOS ONE,2012,7(11).
APA Wei, Na.,Zhang, Lei.,Huang, Huang.,Chen, Yue.,Zheng, Jie.,...&Liang, Zicai.(2012).siRNA Has Greatly Elevated Mismatch Tolerance at 3 ′-UTR Sites.PLOS ONE,7(11).
MLA Wei, Na,et al."siRNA Has Greatly Elevated Mismatch Tolerance at 3 ′-UTR Sites".PLOS ONE 7.11(2012).
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