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学科主题: 药学
题名:
siRNA Has Greatly Elevated Mismatch Tolerance at 3 ′-UTR Sites
作者: Wei, Na1; Zhang, Lei1; Huang, Huang1; Chen, Yue2; Zheng, Jie1; Zhou, Xiao1; Yi, Fan1; Du, Quan1,2; Liang, Zicai1
刊名: PLOS ONE
发表日期: 2012-11-08
DOI: 10.1371/journal.pone.0049309
卷: 7, 期:11
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Multidisciplinary Sciences
研究领域[WOS]: Science & Technology - Other Topics
关键词[WOS]: RNAI ENZYME COMPLEX ; DOUBLE-STRANDED-RNA ; MESSENGER-RNAS ; CAENORHABDITIS-ELEGANS ; GENE-REGULATION ; AGO PROTEINS ; TARGET RNA ; ARGONAUTE ; INTERFERENCE ; MICRORNA
英文摘要:

It has been noted that target sites located in the coding region or the 3′-untranslated region (3′-UTR) can be silenced to significantly different levels by the same siRNA, but little is known about at what specificity the silencing was achieved. In an exploration of positional effects on siRNA specificity by luciferase reporter system, we surprisingly discovered that siRNA had greatly elevated tolerance towards mismatches in target sites in the 3′-UTR of the mRNA compared with the same target sites cloned in the coding region. Assessment of changes in protein and mRNA levels suggested that the differential mismatch tolerance might have resulted from location-specific translational repression in the 3′-UTR. Ablation of argonaute proteins by AGO-specific siRNAs revealed that the AGO2 had major impact on siRNA silencing activity against sites in both coding region and 3′-UTR, while the silencing of nonnucleolytic AGO proteins (AGO1, AGO3 and AGO4) did not significantly affect silencing of sites in either region. This paper revealed the discovery that the specificity of an siRNA can be affected by the location of its target site. Citation: Wei N, Zhang L, Huang H, Chen Y, Zheng J, et al. (2012) siRNA Has Greatly Elevated Mismatch Tolerance at 3′-UTR Sites. PLoS ONE 7(11): e49309. doi:10.1371/journal.pone.0049309

语种: 英语
所属项目编号: 30871385 ; 30873187 ; 5092011 ; 2011CBA01102 ; 10411969100 ; 20090001110052 ; 200800010019
项目资助者: National Natural Science Foundation of China ; Beijing Natural Science Foundation ; National Basic Research Program of China ; Shanghai Science and Technology Committee ; Department of Education of China
WOS记录号: WOS:000312269500101
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/61149
Appears in Collections:北京大学药学院_期刊论文

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作者单位: 1.Peking Univ, Inst Mol Med, Beijing 100871, Peoples R China
2.Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100871, Peoples R China

Recommended Citation:
Wei, Na,Zhang, Lei,Huang, Huang,et al. siRNA Has Greatly Elevated Mismatch Tolerance at 3 ′-UTR Sites[J]. PLOS ONE,2012,7(11).
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