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Development and Validation of a Sensitive Liquid Chromatography-Tandem Mass Spectrometry Method for the Determination of Naringin and Its Metabolite, Naringenin, in Human Plasma
Xiong, Xin1,2; Jiang, Junjie3; Duan, Jingli1; Xie, Yanming3; Wang, Jiannong4; Zhai, Suodi1
关键词`
刊名JOURNAL OF CHROMATOGRAPHIC SCIENCE
2014-08-01
DOI10.1093/chromsci/bmt095
52期:7页:654-660
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]RAT ; RABBITS
英文摘要

A sensitive and specific method was developed for the simultaneous determination of naringin and its metabolite, naringenin, in human plasma by liquid chromatography-tandem mass spectrometry. Hesperidin was used as the internal standard, plasma samples were extracted with ethyl acetate and the analytes were chromatographically separated by using acetonitrile-0.1% formic acid (gradient elution) as the mobile phase. Detection was performed by electrospray ionization mass spectrometry in negative ion mode with multiple reaction monitoring. The lower limit of quantification was 0.5 ng/mL for naringin and naringenin and the linear calibration curves ranged from 0.5 to 200 ng/mL. The intra-run and inter-run precision values were within 8.6 and 7.7% for naringin and between 13.1 and 10.3% for naringenin. The accuracy ranged from 91.3 to 98.2% for naringin and from 90.2 to 97.6 % for naringenin. The validated method was successfully applied to determine concentrations of naringin and naringenin in clinical patients.

语种英语
WOS记录号WOS:000359458500001
项目编号2009ZX09502-030
资助机构National Major Special Project on Invention and Production of New Drugs ; Research on Key Techniques for Postmarketing Reevaluation of Chinese Medicine
引用统计
被引频次:2[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/61151
专题北京大学第三临床医学院_药剂科
作者单位1.Peking Univ, Hosp 3, Dept Pharm, Beijing 100191, Peoples R China
2.Peking Univ, Therapeut Drug Monitoring & Clin Toxicol Ctr, Beijing 100191, Peoples R China
3.China Acad Chinese Med Sci, Inst Basic Res Clin Med, Beijing 100700, Peoples R China
4.China Acad Chinese Med Sci, Xiyuan Hosp, Beijing 100700, Peoples R China
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GB/T 7714
Xiong, Xin,Jiang, Junjie,Duan, Jingli,et al. Development and Validation of a Sensitive Liquid Chromatography-Tandem Mass Spectrometry Method for the Determination of Naringin and Its Metabolite, Naringenin, in Human Plasma[J]. JOURNAL OF CHROMATOGRAPHIC SCIENCE,2014,52(7):654-660.
APA Xiong, Xin,Jiang, Junjie,Duan, Jingli,Xie, Yanming,Wang, Jiannong,&Zhai, Suodi.(2014).Development and Validation of a Sensitive Liquid Chromatography-Tandem Mass Spectrometry Method for the Determination of Naringin and Its Metabolite, Naringenin, in Human Plasma.JOURNAL OF CHROMATOGRAPHIC SCIENCE,52(7),654-660.
MLA Xiong, Xin,et al."Development and Validation of a Sensitive Liquid Chromatography-Tandem Mass Spectrometry Method for the Determination of Naringin and Its Metabolite, Naringenin, in Human Plasma".JOURNAL OF CHROMATOGRAPHIC SCIENCE 52.7(2014):654-660.
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