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学科主题: 临床医学
题名:
Development and Validation of a Sensitive Liquid Chromatography-Tandem Mass Spectrometry Method for the Determination of Naringin and Its Metabolite, Naringenin, in Human Plasma
作者: Xiong, Xin1,2; Jiang, Junjie3; Duan, Jingli1; Xie, Yanming3; Wang, Jiannong4; Zhai, Suodi1
关键词: `
刊名: JOURNAL OF CHROMATOGRAPHIC SCIENCE
发表日期: 2014-08-01
DOI: 10.1093/chromsci/bmt095
卷: 52, 期:7, 页:654-660
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]: RAT ; RABBITS
英文摘要:

A sensitive and specific method was developed for the simultaneous determination of naringin and its metabolite, naringenin, in human plasma by liquid chromatography-tandem mass spectrometry. Hesperidin was used as the internal standard, plasma samples were extracted with ethyl acetate and the analytes were chromatographically separated by using acetonitrile-0.1% formic acid (gradient elution) as the mobile phase. Detection was performed by electrospray ionization mass spectrometry in negative ion mode with multiple reaction monitoring. The lower limit of quantification was 0.5 ng/mL for naringin and naringenin and the linear calibration curves ranged from 0.5 to 200 ng/mL. The intra-run and inter-run precision values were within 8.6 and 7.7% for naringin and between 13.1 and 10.3% for naringenin. The accuracy ranged from 91.3 to 98.2% for naringin and from 90.2 to 97.6 % for naringenin. The validated method was successfully applied to determine concentrations of naringin and naringenin in clinical patients.

语种: 英语
所属项目编号: 2009ZX09502-030
项目资助者: National Major Special Project on Invention and Production of New Drugs ; Research on Key Techniques for Postmarketing Reevaluation of Chinese Medicine
WOS记录号: WOS:000359458500001
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/61151
Appears in Collections:北京大学第三临床医学院_药剂科_期刊论文

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作者单位: 1.Peking Univ, Hosp 3, Dept Pharm, Beijing 100191, Peoples R China
2.Peking Univ, Therapeut Drug Monitoring & Clin Toxicol Ctr, Beijing 100191, Peoples R China
3.China Acad Chinese Med Sci, Inst Basic Res Clin Med, Beijing 100700, Peoples R China
4.China Acad Chinese Med Sci, Xiyuan Hosp, Beijing 100700, Peoples R China

Recommended Citation:
Xiong, Xin,Jiang, Junjie,Duan, Jingli,et al. Development and Validation of a Sensitive Liquid Chromatography-Tandem Mass Spectrometry Method for the Determination of Naringin and Its Metabolite, Naringenin, in Human Plasma[J]. JOURNAL OF CHROMATOGRAPHIC SCIENCE,2014,52(7):654-660.
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