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学科主题: 临床医学
题名:
Apelin activates the expression of inflammatory cytokines in microglial BV2 cells via PI-3K/Akt and MEK/Erk pathways
作者: Chen Li1,2,3; Tao Yong1,2,3; Jiang YanRong1,2,3
关键词: apelin ; inflammation cytokines ; BV2 ; PI-3K/Akt ; MEK/Erk
刊名: SCIENCE CHINA-LIFE SCIENCES
发表日期: 2015-06-01
DOI: 10.1007/s11427-015-4861-0
卷: 58, 期:6, 页:531-540
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biology
研究领域[WOS]: Life Sciences & Biomedicine - Other Topics
关键词[WOS]: PROLIFERATIVE DIABETIC-RETINOPATHY ; RETINAL ENDOTHELIAL-CELLS ; VASCULAR DEVELOPMENT ; ANGIOGENIC FACTOR ; PROTEIN-KINASES ; MULLER CELLS ; INHIBITION ; RECEPTOR ; HYPOXIA ; MICE
英文摘要:

This paper aims to observe the changes of the inflammatory cytokines in microglial BV2 cells stimulated by apelin, and investigate the mechanism of inflammatory cytokines secretion after apelin stimulation. Immunofluorescence and quantitative real-time PCR were performed to observe expression of TNF-alpha, IL-1 beta, IL-10, MIP-1 alpha, and MCP-1 in BV2 cells. Western blot was used to investigate the expression of phosphorylation PI-3K/Akt and phosphorylation Erk signaling pathways in BV2 cells after stimulation by apelin. Furthermore, PI-3K/Akt inhibitor (LY294402) and Erk inhibitor (U0126) were used as antagonists to detect the secretion mechanisms of cytokines in BV2 cells stimulated by apelin. Exogenous recombinant apelin activated the expression of TNF-alpha, IL-1 beta, MCP-1 and MIP-1 alpha in BV2 cells by the detection of fluorescence expression and mRNA. Apelin also unregulated the protein expression of p-PI-3K/Akt and p-Erk in BV2 cells induced by apelin. LY294002 and U0126 inhibited activation of p-PI-3K/Akt and p-Erk expression by Western blot and attenuated the expression of inflammation factors in BV2 cells by fluorescence staining. This study demonstrates that apelin is a potential activator of inflammation factors through the PI3K/Akt and Erk signaling pathway and is potential therapeutically relevant to inflammatory responses of microglia cells.

语种: 英语
所属项目编号: 90561 ; 94410
项目资助者: European Foundation for the Study of Diabetes/Chinese Diabetes Society/Lilly
WOS记录号: WOS:000356518000003
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/61162
Appears in Collections:北京大学第二临床医学院_眼科_期刊论文

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作者单位: 1.Peking Univ, Peoples Hosp, Dept Ophthalmol, Beijing 100044, Peoples R China
2.Minist Educ, Key Lab Vis Loss & Restorat, Beijing 100044, Peoples R China
3.Beijing Key Lab Diag & Therapy Retinal & Choroid, Beijing 100044, Peoples R China

Recommended Citation:
Chen Li,Tao Yong,Jiang YanRong. Apelin activates the expression of inflammatory cytokines in microglial BV2 cells via PI-3K/Akt and MEK/Erk pathways[J]. SCIENCE CHINA-LIFE SCIENCES,2015,58(6):531-540.
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