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Apelin activates the expression of inflammatory cytokines in microglial BV2 cells via PI-3K/Akt and MEK/Erk pathways
Chen Li1,2,3; Tao Yong1,2,3; Jiang YanRong1,2,3
关键词apelin inflammation cytokines BV2 PI-3K/Akt MEK/Erk
刊名SCIENCE CHINA-LIFE SCIENCES
2015-06-01
DOI10.1007/s11427-015-4861-0
58期:6页:531-540
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biology
研究领域[WOS]Life Sciences & Biomedicine - Other Topics
关键词[WOS]PROLIFERATIVE DIABETIC-RETINOPATHY ; RETINAL ENDOTHELIAL-CELLS ; VASCULAR DEVELOPMENT ; ANGIOGENIC FACTOR ; PROTEIN-KINASES ; MULLER CELLS ; INHIBITION ; RECEPTOR ; HYPOXIA ; MICE
英文摘要

This paper aims to observe the changes of the inflammatory cytokines in microglial BV2 cells stimulated by apelin, and investigate the mechanism of inflammatory cytokines secretion after apelin stimulation. Immunofluorescence and quantitative real-time PCR were performed to observe expression of TNF-alpha, IL-1 beta, IL-10, MIP-1 alpha, and MCP-1 in BV2 cells. Western blot was used to investigate the expression of phosphorylation PI-3K/Akt and phosphorylation Erk signaling pathways in BV2 cells after stimulation by apelin. Furthermore, PI-3K/Akt inhibitor (LY294402) and Erk inhibitor (U0126) were used as antagonists to detect the secretion mechanisms of cytokines in BV2 cells stimulated by apelin. Exogenous recombinant apelin activated the expression of TNF-alpha, IL-1 beta, MCP-1 and MIP-1 alpha in BV2 cells by the detection of fluorescence expression and mRNA. Apelin also unregulated the protein expression of p-PI-3K/Akt and p-Erk in BV2 cells induced by apelin. LY294002 and U0126 inhibited activation of p-PI-3K/Akt and p-Erk expression by Western blot and attenuated the expression of inflammation factors in BV2 cells by fluorescence staining. This study demonstrates that apelin is a potential activator of inflammation factors through the PI3K/Akt and Erk signaling pathway and is potential therapeutically relevant to inflammatory responses of microglia cells.

语种英语
WOS记录号WOS:000356518000003
引用统计
被引频次:12[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/61162
专题北京大学第二临床医学院_眼科
作者单位1.Peking Univ, Peoples Hosp, Dept Ophthalmol, Beijing 100044, Peoples R China
2.Minist Educ, Key Lab Vis Loss & Restorat, Beijing 100044, Peoples R China
3.Beijing Key Lab Diag & Therapy Retinal & Choroid, Beijing 100044, Peoples R China
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GB/T 7714
Chen Li,Tao Yong,Jiang YanRong. Apelin activates the expression of inflammatory cytokines in microglial BV2 cells via PI-3K/Akt and MEK/Erk pathways[J]. SCIENCE CHINA-LIFE SCIENCES,2015,58(6):531-540.
APA Chen Li,Tao Yong,&Jiang YanRong.(2015).Apelin activates the expression of inflammatory cytokines in microglial BV2 cells via PI-3K/Akt and MEK/Erk pathways.SCIENCE CHINA-LIFE SCIENCES,58(6),531-540.
MLA Chen Li,et al."Apelin activates the expression of inflammatory cytokines in microglial BV2 cells via PI-3K/Akt and MEK/Erk pathways".SCIENCE CHINA-LIFE SCIENCES 58.6(2015):531-540.
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