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学科主题: 药学
题名:
Simultaneous determination of the active ingredients in Abelmoschus manihot (L.) Medicus by CZE
作者: Liu, Yi2; Li, Wujie1; Ling, Xiaomei1; Lai, Xianyin3; Li, Yuzhen2; Zhang, Qingying3; Zhao, Yuying3
关键词: capillary zone electrophoresis ; flavonoids ; Abelmoschus manihot (L) Medicus
刊名: CHROMATOGRAPHIA
发表日期: 2008-05-01
DOI: 10.1365/s10337-008-0564-z
卷: 67, 期:9-10, 页:819-823
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]: CAPILLARY-ELECTROPHORESIS ; ELECTROCHEMICAL DETECTION ; FLAVONOIDS ; SEPARATION ; RUTIN ; QUERCETIN ; FLOWERS
英文摘要:

A capillary zone electrophoretic method has been developed for the quantitative analysis of four active compounds, quercetin-3-O-robinobioside (LXY3), hyperin (LXY4), isoquercetin (LXY5) and myricetin (LXY7) in A. manihot (L.) Medicus with UV detection at 254 nm. The capillary temperature was kept constant at 25 degrees C. The effects of buffer composition, pH, and concentration and running voltage on migration were studied systematically. Optimum separation was achieved with 20 mmol L(-1) Borax-NaOH buffer (pH 9.50) at 25 degrees C and 25 kV. Regression equations revealed good linear relationship between the peak area of each compound and its concentration. All the correlation coefficients were higher than 0.9990. The relative standard deviations of migration time and the peak area were < 0.53% and 5.34% (inter-day), and < 0.56% and 3.81% (intra-day). The contents of the four compounds in A. manihot (L.) Medicus were successfully determined with satisfactory repeatability and recovery.

语种: 英语
WOS记录号: WOS:000255751100024
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/61163
Appears in Collections:北京大学药学院_化学生物学系_期刊论文

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作者单位: 1.Peking Univ, Dept Pharmaceut Anal, Sch Pharmaceut Sci, Beijing 100083, Peoples R China
2.Peking Univ, Peoples Hosp, Dept Pharm, Beijing 100044, Peoples R China
3.Peking Univ, Dept Nat Med, Sch Pharmaceut Sci, Beijing 100083, Peoples R China

Recommended Citation:
Liu, Yi,Li, Wujie,Ling, Xiaomei,et al. Simultaneous determination of the active ingredients in Abelmoschus manihot (L.) Medicus by CZE[J]. CHROMATOGRAPHIA,2008,67(9-10):819-823.
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