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学科主题: 药学
题名:
Solid-phase extraction-liquid chromatographic method for the determination and pharmacokinetic studies, of albiflorin and paeoniflorin in rat serum after oral administration of Si-Wu decoction
作者: Sheng, YX; Li, L; Wang, C; Li, YY; Guo, D
关键词: albiflorin ; paeoniflorin
刊名: JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
发表日期: 2004-07-05
DOI: 10.1016/j.jchromb.2004.03.041
卷: 806, 期:2, 页:127-132
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]: PAEONIAE RADIX ; GLYCYRRHIZIN ; CONSTITUENTS ; ROOT ; MICE ; PERFORMANCE ; CORTEX ; MUSCLE
英文摘要:

A sensitive and rapid high-performance liquid chromatography (HPLC) method with solid-phase extraction (SPE) to simultaneously determine albiflorin and paeoniflorin in rat serum was described. Serum samples were pretreated with solid-phase extraction using Extract-Clean(TM) cartridges, and the extracts were analyzed by HPLC on a reversed-phase C-18 column and a mobile phase of acetonitrile-0.03% formic acid (17:83 (v/v)) with ultraviolet detection at 230 nm. Pentoxifylline was used as the internal standard (IS). The linear ranges of the calibration curves were 29-1450 ng/ml for albiflorin and 10-2000 ng/ml for paeoniflorin. The intra- and inter-day precisions (R.S.D.) were less than or equal to10.49% for albiflorin and less than or equal to11.29% for paeoniflorin, respectively. Mean recovery was determined to be 89.75% for albiflorin and 85.82% for paeoniflorin. The limit of quantification was 29 ng/ml for albiflorin and 10 ng/ml for paeoniflorin, respectively. The validated method was applicable to pharmacokinetic studies of albiflorin and paeoniflorin from rat serum after oral administration of Si-Wu decoction. The pharmacokinetic study indicated that albiflorin and paeoniflorin had poor absorption and rapid elimination. This assay result was necessary for the pharmacokinetic evaluation of Si-Wu decoction. (C) 2004 Elsevier B.V. All rights reserved.

语种: 英语
WOS记录号: WOS:000221945700007
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/61174
Appears in Collections:北京大学药学院_期刊论文

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作者单位: 1.Peking Univ, Sch Pharmaceut Sci, Beijing 100083, Peoples R China
2.Peking Univ, Modern Res Ctr Tradit Chinese Med, Beijing 100083, Peoples R China

Recommended Citation:
Sheng, YX,Li, L,Wang, C,et al. Solid-phase extraction-liquid chromatographic method for the determination and pharmacokinetic studies, of albiflorin and paeoniflorin in rat serum after oral administration of Si-Wu decoction[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2004,806(2):127-132.
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