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学科主题: 临床医学
题名:
The lncRNA H19 promotes epithelial to mesenchymal transition by functioning as miRNA sponges in colorectal cancer
作者: Liang, Wei-Cheng1,2; Fu, Wei-Ming3; Wong, Cheuk-Wa1,2; Wang, Yan1; Wang, Wei-Mao1; Hu, Guo-Xin4; Zhang, Li1; Xiao, Li-Jia5; Wan, David Chi-Cheong1; Zhang, Jin-Fang1,6; Waye, Mary Miu-Yee1,2
关键词: miRNA sponges ; lncRNA ; ceRNA
刊名: ONCOTARGET
发表日期: 2015-09-08
DOI: 10.18632/oncotarget.4154
卷: 6, 期:26, 页:22513-22525
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology ; Cell Biology
研究领域[WOS]: Oncology ; Cell Biology
关键词[WOS]: LONG NONCODING RNA ; TUMOR PROGRESSION ; STEM-CELLS ; E-CADHERIN ; BLADDER-CANCER ; MIR-200 FAMILY ; METASTASIS ; EXPRESSION ; PHENOTYPE ; CARCINOMA
英文摘要:

Recently, the long non-coding RNA (lncRNA) H19 has been identified as an oncogenic gene in multiple cancer types and elevated expression of H19 was tightly linked to tumorigenesis and cancer progression. However, the molecular basis for this observation has not been characterized in colorectal cancer (CRC) especially during epithelial to mesenchymal transition (EMT) progression. In our studies, H19 was characterized as a novel regulator of EMT in CRC. We found that H19 was highly expressed in mesenchymal-like cancer cells and primary CRC tissues. Stable expression of H19 significantly promotes EMT progression and accelerates in vivo and in vitro tumor growth. Furthermore, by using bioinformatics study and RNA immunoprecipitation combined with luciferase reporter assays, we demonstrated that H19 functioned as a competing endogenous RNA (ceRNA) for miR-138 and miR-200a, antagonized their functions and led to the de-repression of their endogenous targets Vimentin, ZEB1, and ZEB2, all of which were core marker genes for mesenchymal cells. Taken together, these observations imply that the lncRNA H19 modulated the expression of multiple genes involved in EMT by acting as a competing endogenous RNA, which may build up the missing link between the regulatory miRNA network and EMT progression.

语种: 英语
所属项目编号: 6903642 ; 81201699 ; 81372450
项目资助者: Health and Medical Research Fund of Hong Kong ; National Natural Science Foundation of China
WOS记录号: WOS:000362954800073
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/61188
Appears in Collections:北京大学深圳医院_感染性疾病科_期刊论文

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作者单位: 1.Chinese Univ Hong Kong, Sch Biomed Sci, Shatin, Hong Kong, Peoples R China
2.Chinese Univ Hong Kong, Croucher Lab Human Genom, Shatin, Hong Kong, Peoples R China
3.Chinese Acad Sci, Guangzhou Inst Adv Technol, Guangzhou, Guangdong, Peoples R China
4.Peking Univ, Shenzhen Hosp, Dept Infect Dis, Shenzhen, Peoples R China
5.Guangdong Med Coll, Nanshan Affiliated Hosp, Dept Clin Lab, Shenzhen, Peoples R China
6.Chinese Univ Hong Kong, Prince Wales Hosp, Dept Orthopaed & Traumatol, Shatin, Hong Kong, Peoples R China

Recommended Citation:
Liang, Wei-Cheng,Fu, Wei-Ming,Wong, Cheuk-Wa,et al. The lncRNA H19 promotes epithelial to mesenchymal transition by functioning as miRNA sponges in colorectal cancer[J]. ONCOTARGET,2015,6(26):22513-22525.
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