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学科主题: 基础医学
题名:
Restoration in vitro of impaired T-cell responses in patients with chronic hepatitis B by autologous dendritic cells loaded with hepatitis B virus proteins (R2)
作者: Duan, XZ; He, HX; Zhuang, H
关键词: ELISPOT ; HBcAg ; HBsAg ; vaccine
刊名: JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY
发表日期: 2006-06-01
DOI: 10.1111/j.1440-1746.2006.04262.x
卷: 21, 期:6, 页:970-976
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Gastroenterology & Hepatology
研究领域[WOS]: Gastroenterology & Hepatology
关键词[WOS]: TRANSGENIC MICE ; INFECTION ; MATURATION ; GENERATION ; BLOOD ; IMMUNOTHERAPY ; TOLERANCE ; MELANOMA ; IMMUNITY ; DISEASES
英文摘要:

Background: The purpose of the present paper was to investigate dendritic cell (DC) and T-cell functions in patients with chronic hepatitis B (CHB) and determine whether therapeutic DC vaccines could restore T-cell function in those patients in vitro.

Methods: Twelve patients with CHB and 10 normal control subjects with positivity for antibodies to hepatitis B surface and core antigens (anti-HBs and anti-HBc positivity) were enrolled in the present study. Phenotype analysis and allogeneic mixed lymphocyte reaction assay of DC from CHB patients and normal controls were made in the absence or presence of a cocktail of cytokines: interleukin-1 beta (IL-1 beta), prostaglandin E-2 (PGE(2)), IL-6 and tumor necrosis factor-alpha (TNF-alpha). Autologous T-cell proliferation assays and the enzyme-linked immunospot (ELISPOT) method for detecting interferon-gamma (IFN-gamma)-producing CD8(+) T cells were used to evaluate the efficacy of DC loaded in vitro with HBsAg or HBcAg.

Results: The DC from CHB patients had a lower expression of costimulatory molecules CD80, CD86 and impaired allogeneic mixed lymphocyte reaction capacity compared to those from normal controls. However, the impaired DC function could be restored partially by cytokine cocktail supplemented in vitro. Mature DC loaded with HBsAg or HBcAg showed a greater capacity for autologous T-cell proliferation and antigen-specific IFN-gamma production than immature DC. Moreover, as a DC -loading antigen, HBcAg was more immunogenic than HBsAg.

Conclusions: The impaired function of DC in patients with CHB may be restored by supplementation in vitro with a cocktail of cytokines, and therapeutic DC vaccines might be effective to treat CHB infection in humans.

语种: 英语
WOS记录号: WOS:000237740100007
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/61195
Appears in Collections:基础医学院_病原生物学系_期刊论文

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作者单位: Peking Univ, Hlth Sci Ctr, Dept Microbiol, Beijing 100083, Peoples R China

Recommended Citation:
Duan, XZ,He, HX,Zhuang, H. Restoration in vitro of impaired T-cell responses in patients with chronic hepatitis B by autologous dendritic cells loaded with hepatitis B virus proteins (R2)[J]. JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY,2006,21(6):970-976.
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