北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学第一临床医学院  > 肾脏内科  > 期刊论文
学科主题: 临床医学
题名:
The Role for HNF-1 beta-Targeted Collectrin in Maintenance of Primary Cilia and Cell Polarity in Collecting Duct Cells
作者: Zhang, Yanling1,2; Wada, Jun1; Yasuhara, Akihiro1; Iseda, Izumi1; Eguchi, Jun1; Fukui, Kenji3; Yang, Qin3,4; Yamagata, Kazuya3; Hiesberger, Thomas5,6; Igarashi, Peter5,6; Zhang, Hong7; Wang, Haiyan7; Akagi, Shigeru8; Kanwar, Yashpal S.8; Makino, Hirofumi1
刊名: PLOS ONE
发表日期: 2007-05-02
DOI: 10.1371/journal.pone.0000414
卷: 2, 期:5
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Multidisciplinary Sciences
研究领域[WOS]: Science & Technology - Other Topics
英文摘要:

Collectrin, a homologue of angiotensin converting enzyme 2 (ACE2), is a type I transmembrane protein, and we originally reported its localization to the cytoplasm and apical membrane of collecting duct cells. Recently, two independent studies of targeted disruption of collectrin in mice resulted in severe and general defects in renal amino acid uptake. Collectrin has been reported to be under the transcriptional regulation by HNF-1 alpha, which is exclusively expressed in proximal tubules and localized at the luminal side of brush border membranes. The deficiency of collectrin was associated with reduction of multiple amino acid transporters on luminal membranes. In the current study, we describe that collectrin is a target of HNF-1 beta and heavily expressed in the primary cilium of renal collecting duct cells. Collectrin is also localized in the vesicles near the peri-basal body region and binds to gamma-actin-myosin II-A, SNARE, and polycystin-2-polaris complexes, and all of these are involved in intracellular and ciliary movement of vesicles and membrane proteins. Treatment of mIMCD3 cells with collectrin siRNA resulted in defective cilium formation, increased cell proliferation and apoptosis, and disappearance of polycystin-2 in the primary cilium. Suppression of collectrin mRNA in metanephric culture resulted in the formation of multiple longitudinal cysts in ureteric bud branches. Taken together, the cystic change and formation of defective cilium with the interference in the collectrin functions would suggest that it is necessary for recycling of the primary cilia-specific membrane proteins, the maintenance of the primary cilia and cell polarity of collecting duct cells. The transcriptional hierarchy between HNF-1 beta and PKD (polycystic kidney disease) genes expressed in the primary cilia of collecting duct cells has been suggested, and collectrin is one of such HNF-1 beta regulated genes.

语种: 英语
所属项目编号: 17590829 ; 18390249 ; R01DK42921 ; R01DK67565 ; DK28492 ; DK60635 ; 1604241
项目资助者: JSPS (Japan Society for the Promotion of Science) ; Ministry of Education, Science and Culture, Japan ; Uehara Memorial Foundation ; Scientific Research, Ministry of Education, Science and Culture, Japan ; NIH
WOS记录号: WOS:000207445700016
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/61197
Appears in Collections:北京大学第一临床医学院_肾脏内科_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Okayama Univ, Grad Sch Med, Dept Med & Clin Sci, Okayama, Japan
2.Hebei Med Univ, Hosp 3, Dept Nephrol, Shijiazhuang, Peoples R China
3.Osaka Univ, Grad Sch Med, Dept Metab Med, Osaka, Japan
4.Harvard Univ, Beth Israel Deaconess Med Ctr, Sch Med, Div Endocrinol Diabet & Metab, Boston, MA 02215 USA
5.Univ Texas SW Med Ctr Dallas, Dept Internal Med, Dallas, TX 75390 USA
6.Univ Texas SW Med Ctr Dallas, Div Basic Sci, Dallas, TX 75390 USA
7.Peking Univ, Hosp 1, Inst Nephrol, Div Renal, Beijing 100871, Peoples R China
8.Northwestern Univ, Dept Pathol, Feinberg Sch Med, Chicago, IL 60611 USA

Recommended Citation:
Zhang, Yanling,Wada, Jun,Yasuhara, Akihiro,et al. The Role for HNF-1 beta-Targeted Collectrin in Maintenance of Primary Cilia and Cell Polarity in Collecting Duct Cells[J]. PLOS ONE,2007,2(5).
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Zhang, Yanling]'s Articles
[Wada, Jun]'s Articles
[Yasuhara, Akihiro]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Zhang, Yanling]‘s Articles
[Wada, Jun]‘s Articles
[Yasuhara, Akihiro]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace