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学科主题: 基础医学
题名:
Comprehensive characterization of expression patterns of protein 4.1 family members in mouse adrenal gland: implications for functions
作者: Wang, Hua1,2; Liu, Congrong1,2; Debnath, Gargi1; Baines, Anthony J.4; Conboy, John G.5; Mohandas, Narla1; An, Xiuli1,3
关键词: 4 ; 1R ; 4 ; 1G ; 4 ; 1B ; 4 ; 1N ; Adrenal gland
刊名: HISTOCHEMISTRY AND CELL BIOLOGY
发表日期: 2010-10-01
DOI: 10.1007/s00418-010-0749-z
卷: 134, 期:4, 页:411-420
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Cell Biology ; Microscopy
研究领域[WOS]: Cell Biology ; Microscopy
关键词[WOS]: ACTIN-BINDING DOMAIN ; CELL MEMBRANE ; GENE FAMILY ; CYTOSKELETAL ; COMPLEX ; ISOFORMS ; MECHANOCHEMISTRY ; EVOLUTION ; INTEGRITY ; JUNCTION
英文摘要:

The members of the protein 4.1 family, 4.1R, 4.1G, 4.1N, and 4.1B, are encoded by four genes, all of which undergo complex alternative splicing. It is well established that 4.1R, the prototypical member of the family, serves as an adapter that links the spectrin-actin based cytoskeleton to the plasma membrane in red cells. It is required for mechanical resilience of the membrane, and it ensures the cell surface accumulation of selected membrane proteins. However, the function of 4.1 proteins outside erythrocytes remains under-explored, especially in endocrine tissues. Transcripts of all 4.1 homologs have previously been documented to be abundantly expressed in adrenal gland. In order to begin to decipher the function of 4.1 proteins in adrenal gland, we performed a detailed characterization of the expression pattern of various 4.1 proteins and their cellular localization. We show that 4.1R (similar to 80 and similar to 135 kDa) splice forms are expressed on the membrane of all cells, while a similar to 160 kDa 4.1G splice form is distributed in the cytoplasm and the membrane of zona glomerulosa and of medullary cells. Two 4.1N splice forms, similar to 135 and similar to 95 kDa, are present in the peri-nuclear region of both zona glomerulosa and medullary cells, while a single similar to 130 kDa 4.1B splice form, is detected in all layers of adrenal gland in both the cytoplasm and the membrane. The characterization of distinct splice forms of various 4.1 proteins with diverse cellular and sub-cellular localization indicates multiple functions for this family of proteins in endocrine functions of adrenal gland.

语种: 英语
所属项目编号: DK 32094 ; DK 26263
项目资助者: NIH
WOS记录号: WOS:000282974600010
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/61219
Appears in Collections:基础医学院_病理学系_期刊论文

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作者单位: 1.New York Blood Ctr, Red Cell Physiol Lab, New York, NY 10065 USA
2.Peking Univ, Hlth Sci Ctr, Dept Pathol, Beijing 100191, Peoples R China
3.Peking Univ, Hlth Sci Ctr, Dept Biophys, Beijing 100191, Peoples R China
4.Univ Kent, Sch Biosci, Canterbury CT2 7NJ, Kent, England
5.Univ Calif Berkeley, Lawrence Berkeley Lab, Div Life Sci, Berkeley, CA 94720 USA

Recommended Citation:
Wang, Hua,Liu, Congrong,Debnath, Gargi,et al. Comprehensive characterization of expression patterns of protein 4.1 family members in mouse adrenal gland: implications for functions[J]. HISTOCHEMISTRY AND CELL BIOLOGY,2010,134(4):411-420.
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