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学科主题: 口腔医学
题名:
Decline of superoxide dismutase activity during antioxidant-induced apoptosis in HL-60 cells
作者: Zhang, W; Hashimoto, K; Yu, GY; Sakagami, H
关键词: antioxidant ; prooxidant action ; HL-60 ; apoptosis ; DNA fragmentation ; mitochondria ; MnSOD
刊名: ANTICANCER RESEARCH
发表日期: 2002
卷: 22, 期:1A, 页:219-224
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology
研究领域[WOS]: Oncology
关键词[WOS]: HYDROGEN-PEROXIDE ; INDUCTION ; RESISTANCE ; ASCORBATE
英文摘要:

Possible changes in Mn-containing superoxide dismutase (MnSOD) and Cu- and Zn-containing superoxide dismutase (CuZnSOD) activity during the apoptosis of human promyelocytic leukemia HL-60 cells, induced by three antioxidants, were investigated. Agarose gel electrophoresis of the DNA isolated from cells treated with sodium ascorbate, gallic acid or epigallocatechin gallate (EGCG) showed the internucleosomal DNA fragmentation, a biochemical hallmark of apoptosis. The optimum concentration for induction of DNA fragmentation was narrow and higher concentrations were rather inhibitory. Their effects were detected after 3 hours and reached a maximum level at 6 hours. Polyactylamide gel electrophoresis and activity staining demonstrated that both MnSOD and CuZnSOD activities were significantly reduced at cytotoxic concentrations of all these compounds. Incubation of an intracellular MnSOD-enriched fraction with apoptosis-inducing concentrations of sodium ascorbate, gallic acid or EGCG did not significantly reduce the MnSOD activity, suggesting that their actions might be cell-mediated. These data suggest the mitochondrial dysfunction at the early stages of apoptosis.

语种: 英语
WOS记录号: WOS:000175351900035
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/61223
Appears in Collections:北京大学口腔医学院_期刊论文

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作者单位: 1.Meikai Univ, Sch Dent, Dept Dent Pharmacol, Sakado, Saitama 3500283, Japan
2.Meikai Univ, Sch Dent, Meikai Pharmaco Med Lab, Sakado, Saitama 3500283, Japan
3.Peking Univ, Sch Stomatol, Dept Oral & Maxillofacial Surg, Beijing 100038, Peoples R China

Recommended Citation:
Zhang, W,Hashimoto, K,Yu, GY,et al. Decline of superoxide dismutase activity during antioxidant-induced apoptosis in HL-60 cells[J]. ANTICANCER RESEARCH,2002,22(1A):219-224.
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