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Decline of superoxide dismutase activity during antioxidant-induced apoptosis in HL-60 cells
Zhang, W; Hashimoto, K; Yu, GY; Sakagami, H
关键词antioxidant prooxidant action HL-60 apoptosis DNA fragmentation mitochondria MnSOD
刊名ANTICANCER RESEARCH
2002
22期:1A页:219-224
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Oncology
研究领域[WOS]Oncology
关键词[WOS]HYDROGEN-PEROXIDE ; INDUCTION ; RESISTANCE ; ASCORBATE
英文摘要

Possible changes in Mn-containing superoxide dismutase (MnSOD) and Cu- and Zn-containing superoxide dismutase (CuZnSOD) activity during the apoptosis of human promyelocytic leukemia HL-60 cells, induced by three antioxidants, were investigated. Agarose gel electrophoresis of the DNA isolated from cells treated with sodium ascorbate, gallic acid or epigallocatechin gallate (EGCG) showed the internucleosomal DNA fragmentation, a biochemical hallmark of apoptosis. The optimum concentration for induction of DNA fragmentation was narrow and higher concentrations were rather inhibitory. Their effects were detected after 3 hours and reached a maximum level at 6 hours. Polyactylamide gel electrophoresis and activity staining demonstrated that both MnSOD and CuZnSOD activities were significantly reduced at cytotoxic concentrations of all these compounds. Incubation of an intracellular MnSOD-enriched fraction with apoptosis-inducing concentrations of sodium ascorbate, gallic acid or EGCG did not significantly reduce the MnSOD activity, suggesting that their actions might be cell-mediated. These data suggest the mitochondrial dysfunction at the early stages of apoptosis.

语种英语
WOS记录号WOS:000175351900035
引用统计
被引频次:22[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/61223
专题北京大学口腔医学院
作者单位1.Meikai Univ, Sch Dent, Dept Dent Pharmacol, Sakado, Saitama 3500283, Japan
2.Meikai Univ, Sch Dent, Meikai Pharmaco Med Lab, Sakado, Saitama 3500283, Japan
3.Peking Univ, Sch Stomatol, Dept Oral & Maxillofacial Surg, Beijing 100038, Peoples R China
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GB/T 7714
Zhang, W,Hashimoto, K,Yu, GY,et al. Decline of superoxide dismutase activity during antioxidant-induced apoptosis in HL-60 cells[J]. ANTICANCER RESEARCH,2002,22(1A):219-224.
APA Zhang, W,Hashimoto, K,Yu, GY,&Sakagami, H.(2002).Decline of superoxide dismutase activity during antioxidant-induced apoptosis in HL-60 cells.ANTICANCER RESEARCH,22(1A),219-224.
MLA Zhang, W,et al."Decline of superoxide dismutase activity during antioxidant-induced apoptosis in HL-60 cells".ANTICANCER RESEARCH 22.1A(2002):219-224.
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