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学科主题: 基础医学
题名:
Laminar shear stress regulates liver X receptor in vascular endothelial cells
作者: Zhu, Minjia1; Fu, Yi1; Hou, Yingjian1; Wang, Nanping2; Guan, Youfei1; Tang, Chaoshu1; Shyy, John Y. -J.3; Zhu, Yi1
关键词: shear stress ; LXR ; PPAR gamma ; endothelial cells
刊名: ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
发表日期: 2008-03-01
DOI: 10.1161/ATVBAHA.107.143487
卷: 28, 期:3, 页:527-533
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Hematology ; Peripheral Vascular Disease
研究领域[WOS]: Hematology ; Cardiovascular System & Cardiology
关键词[WOS]: PROLIFERATOR-ACTIVATED RECEPTOR ; BINDING CASSETTE TRANSPORTER-1 ; NF-KAPPA-B ; PPAR-GAMMA ; CELLULAR CHOLESTEROL ; LESION FORMATION ; GENE-EXPRESSION ; LXR-ALPHA ; ATHEROSCLEROSIS ; PATHWAY
英文摘要:

Objective-The liver X receptors (LXRs) regulate a set of genes involved in lipid metabolism and reverse cholesterol transport. We investigated the mechanism by which shear stress regulates LXR in vascular endothelial cells (ECs).

Methods and Results-Western blot showed that the protein level of LXR and its target ABCA1 in the mouse thoracic aorta was higher than that in the aortic arch. As well, the mRNA level of LXR and its target genes ABCA1, ABCG1, ApoE, and LPL in the thoracic aorta was higher. In vitro, bovine aortic ECs were subjected to a steady laminar flow (12 dyne/cm(2)). The expressions of LXR and the LXR-mediated transcription were increased by laminar shear stress. Laminar flow increased LXR-ligand binding and the gene expression of sterol 27-hydroxylase (CYP27), which suggests an increased level of LXR ligand in ECs. This effect was attenuated by LXR alpha and CYP27 RNAi. The decrease of LXR in the aorta of PPAR gamma(-/-) mice and that of C57 mice fed with PPAR gamma antagonist suggest the involvement of PPAR gamma in the LXR induction by flow.

Conclusion-Laminar flow increases LXR function via a PPAR gamma-CYP27 dependent mechanism, which reveals an atheroprotective role for laminar flow exerting on endothelium.

语种: 英语
WOS记录号: WOS:000253429000028
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/61227
Appears in Collections:基础医学院_心血管所_期刊论文

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作者单位: 1.Peking Univ, Hlth Sci Ctr, Dept Physiol & Pathophysiol, Beijing 100083, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Key Lab Mol Cardiovasc Sci Educ Minist, Inst Cardiovasc Res, Beijing 100083, Peoples R China
3.Univ Calif Riverside, Div Biomed Sci, Riverside, CA 92521 USA

Recommended Citation:
Zhu, Minjia,Fu, Yi,Hou, Yingjian,et al. Laminar shear stress regulates liver X receptor in vascular endothelial cells[J]. ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY,2008,28(3):527-533.
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