IR@PKUHSC  > 北京大学第二临床医学院  > 心血管内科
学科主题临床医学
Signature of Circulating MicroRNAs as Potential Biomarkers in Vulnerable Coronary Artery Disease
Ren, Jingyi1; Zhang, Jing1; Xu, Ning2; Han, Guanping1; Geng, Qiang1; Song, Junxian1; Li, Sufang1; Zhao, Jianqing3; Chen, Hong1
刊名PLOS ONE
2013-12-05
DOI10.1371/journal.pone.0080738
8期:12
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Multidisciplinary Sciences
研究领域[WOS]Science & Technology - Other Topics
关键词[WOS]GROWTH-FACTOR-BETA ; MICROPARTICLES CORRELATE ; CELLS ; PLAQUE ; INFLAMMATION ; EXPRESSION ; MARKERS ; GLIOMA ; MICE
英文摘要

Aims: MicroRNAs (miRNAs) play important roles in the pathogenesis of cardiovascular diseases. Circulating miRNAs were recently identified as biomarkers for various physiological and pathological conditions. In this study, we aimed to identify the circulating miRNA fingerprint of vulnerable coronary artery disease (CAD) and explore its potential as a novel biomarker for this disease.

Methods and Results: The Taqman low-density miRNA array and coexpression network analyses were used to identify distinct miRNA expression profiles in the plasma of patients with typical unstable angina (UA) and angiographically documented CAD (UA group, n = 13) compared to individuals with non-cardiac chest pain (control group, n = 13). Significantly elevated expression levels of miR-106b/25 cluster, miR-17/92a cluster, miR-21/590-5p family, miR-126*, and miR-451 were observed in UA patients compared to controls. These findings were validated by real-time PCR in another 45 UA patients, 31 stable angina patients, and 37 controls. In addition, miR-106b, miR-25, miR-92a, miR-21, miR-590-5p, miR-126* and miR-451 were upregulated in microparticles (MPs) isolated from the plasma of UA patients (n = 5) compared to controls (n = 5). Using flow cytometry and immunolabeling, we further found that Annexin V+ MPs were increased in the plasma samples of UA patients compared to controls, and the majority of the increased MPs in plasma were shown to be Annexin V+ CD31(+) MPs. The findings suggest that Annexin V+ CD31(+) MPs may contribute to the elevated expression of the selected miRNAs in the circulation of patients with vulnerable CAD.

Conclusion: The circulating miRNA signature, consisting of the miR-106b/25 cluster, miR-17/92a cluster, miR-21/590-5p family, miR-126* and miR-451, may be used as a novel biomarker for vulnerable CAD.

语种英语
WOS记录号WOS:000328566100019
项目编号81270274 ; 7132225 ; 2011-4022-03 ; 81270276 ; 09010060161
资助机构National Natural Science Foundation of China (NSFC) ; Beijing Natural Science Foundation ; Capital Health Research and Development of Special Funds ; NSFC ; Clinical Medicine Research Special Funds from the Chinese Medical Association
引用统计
被引频次:75[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/61255
专题北京大学第二临床医学院_心血管内科
作者单位1.Karolinska Inst, Dept Med, Stockholm, Sweden
2.Natl Engn Res Ctr Beijing Biochip Technol, Beijing, Peoples R China
3.Peking Univ, Peoples Hosp, Dept Cardiol, Beijing 100871, Peoples R China
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GB/T 7714
Ren, Jingyi,Zhang, Jing,Xu, Ning,et al. Signature of Circulating MicroRNAs as Potential Biomarkers in Vulnerable Coronary Artery Disease[J]. PLOS ONE,2013,8(12).
APA Ren, Jingyi.,Zhang, Jing.,Xu, Ning.,Han, Guanping.,Geng, Qiang.,...&Chen, Hong.(2013).Signature of Circulating MicroRNAs as Potential Biomarkers in Vulnerable Coronary Artery Disease.PLOS ONE,8(12).
MLA Ren, Jingyi,et al."Signature of Circulating MicroRNAs as Potential Biomarkers in Vulnerable Coronary Artery Disease".PLOS ONE 8.12(2013).
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