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IR@PKUHSC  > 北京大学第二临床医学院  > 心血管内科  > 期刊论文
学科主题: 临床医学
题名:
Signature of Circulating MicroRNAs as Potential Biomarkers in Vulnerable Coronary Artery Disease
作者: Ren, Jingyi1; Zhang, Jing1; Xu, Ning2; Han, Guanping1; Geng, Qiang1; Song, Junxian1; Li, Sufang1; Zhao, Jianqing3; Chen, Hong1
刊名: PLOS ONE
发表日期: 2013-12-05
DOI: 10.1371/journal.pone.0080738
卷: 8, 期:12
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Multidisciplinary Sciences
研究领域[WOS]: Science & Technology - Other Topics
关键词[WOS]: GROWTH-FACTOR-BETA ; MICROPARTICLES CORRELATE ; CELLS ; PLAQUE ; INFLAMMATION ; EXPRESSION ; MARKERS ; GLIOMA ; MICE
英文摘要:

Aims: MicroRNAs (miRNAs) play important roles in the pathogenesis of cardiovascular diseases. Circulating miRNAs were recently identified as biomarkers for various physiological and pathological conditions. In this study, we aimed to identify the circulating miRNA fingerprint of vulnerable coronary artery disease (CAD) and explore its potential as a novel biomarker for this disease.

Methods and Results: The Taqman low-density miRNA array and coexpression network analyses were used to identify distinct miRNA expression profiles in the plasma of patients with typical unstable angina (UA) and angiographically documented CAD (UA group, n = 13) compared to individuals with non-cardiac chest pain (control group, n = 13). Significantly elevated expression levels of miR-106b/25 cluster, miR-17/92a cluster, miR-21/590-5p family, miR-126*, and miR-451 were observed in UA patients compared to controls. These findings were validated by real-time PCR in another 45 UA patients, 31 stable angina patients, and 37 controls. In addition, miR-106b, miR-25, miR-92a, miR-21, miR-590-5p, miR-126* and miR-451 were upregulated in microparticles (MPs) isolated from the plasma of UA patients (n = 5) compared to controls (n = 5). Using flow cytometry and immunolabeling, we further found that Annexin V+ MPs were increased in the plasma samples of UA patients compared to controls, and the majority of the increased MPs in plasma were shown to be Annexin V+ CD31(+) MPs. The findings suggest that Annexin V+ CD31(+) MPs may contribute to the elevated expression of the selected miRNAs in the circulation of patients with vulnerable CAD.

Conclusion: The circulating miRNA signature, consisting of the miR-106b/25 cluster, miR-17/92a cluster, miR-21/590-5p family, miR-126* and miR-451, may be used as a novel biomarker for vulnerable CAD.

语种: 英语
所属项目编号: 81270274 ; 7132225 ; 2011-4022-03 ; 81270276 ; 09010060161
项目资助者: National Natural Science Foundation of China (NSFC) ; Beijing Natural Science Foundation ; Capital Health Research and Development of Special Funds ; NSFC ; Clinical Medicine Research Special Funds from the Chinese Medical Association
WOS记录号: WOS:000328566100019
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/61255
Appears in Collections:北京大学第二临床医学院_心血管内科_期刊论文

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作者单位: 1.Karolinska Inst, Dept Med, Stockholm, Sweden
2.Natl Engn Res Ctr Beijing Biochip Technol, Beijing, Peoples R China
3.Peking Univ, Peoples Hosp, Dept Cardiol, Beijing 100871, Peoples R China

Recommended Citation:
Ren, Jingyi,Zhang, Jing,Xu, Ning,et al. Signature of Circulating MicroRNAs as Potential Biomarkers in Vulnerable Coronary Artery Disease[J]. PLOS ONE,2013,8(12).
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