IR@PKUHSC  > 北京大学第三临床医学院
学科主题临床医学
The Transcriptome and DNA Methylome Landscapes of Human Primordial Germ Cells
Guo, Fan1,2; Yan, Liying1,2,3; Guo, Hongshan1,2; Li, Lin1,2; Hu, Boqiang1,2; Zhao, Yangyu1,2,3; Yong, Jun1,2; Hu, Yuqiong1,2,5,9; Wang, Xiaoye1,2,3; Wei, Yuan1,2,3; Wang, Wei1,2,3; Li, Rong1,2,3; Yan, Jie1,2,3; Zhi, Xu1,2,3; Zhang, Yan1,2,3; Jin, Hongyan1,2,3; Zhang, Wenxin1,2,3; Hou, Yu1,2; Zhu, Ping1,2,5; Li, Jingyun1,2,5; Zhang, Ling6; Liu, Sirui6; Ren, Yixin1,2,3; Zhu, Xiaohui1,2,3; Wen, Lu1,2,4; Gao, Yi Qin6; Tang, Fuchou1,2,4,5,7; Qiao, Jie1,2,3,5,8
刊名CELL
2015-06-04
DOI10.1016/j.cell.2015.05.015
161期:6页:1437-1452
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Cell Biology
资助者National Natural Science of China ; National Basic Research Program of China ; Beijing Municipal Science and Technology Commission ; Peking University for translation research ; National Natural Science Foundation of China ; Peking-Tsinghua Center for Life Sciences ; Center for Molecular and Translational Medicine ; National Natural Science of China ; National Basic Research Program of China ; Beijing Municipal Science and Technology Commission ; Peking University for translation research ; National Natural Science Foundation of China ; Peking-Tsinghua Center for Life Sciences ; Center for Molecular and Translational Medicine
研究领域[WOS]Biochemistry & Molecular Biology ; Cell Biology
关键词[WOS]EMBRYONIC STEM-CELLS ; SINGLE-CELL ; GENE-EXPRESSION ; DEMETHYLATION ; GENOME ; DYNAMICS ; MICE ; SPECIFICATION ; PATHWAY ; ERASURE
英文摘要

Germ cells are vital for transmitting genetic information from one generation to the next and for maintaining the continuation of species. Here, we analyze the transcriptome of human primordial germ cells (PGCs) from the migrating stage to the gonadal stage at single-cell and single-base resolutions. Human PGCs show unique transcription patterns involving the simultaneous expression of both pluripotency genes and germline-specific genes, with a subset of them displaying developmental-stage-specific features. Furthermore, we analyze the DNA methylome of human PGCs and find global demethylation of their genomes. Approximately 10 to 11 weeks after gestation, the PGCs are nearly devoid of any DNA methylation, with only 7.8% and 6.0% of the median methylation levels in male and female PGCs, respectively. Our work paves the way toward deciphering the complex epigenetic reprogramming of the germline with the aim of restoring totipotency in fertilized oocytes.

语种英语
所属项目编号31230047 ; 31322037 ; 31271543 ; 2011CB944504 ; 2014CB943200 ; 2012CB966704 ; 2011CB966303 ; Z131100005213006 ; 31440063
资助者National Natural Science of China ; National Basic Research Program of China ; Beijing Municipal Science and Technology Commission ; Peking University for translation research ; National Natural Science Foundation of China ; Peking-Tsinghua Center for Life Sciences ; Center for Molecular and Translational Medicine ; National Natural Science of China ; National Basic Research Program of China ; Beijing Municipal Science and Technology Commission ; Peking University for translation research ; National Natural Science Foundation of China ; Peking-Tsinghua Center for Life Sciences ; Center for Molecular and Translational Medicine
WOS记录号WOS:000355935000021
引用统计
被引频次:89[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/61258
专题北京大学第三临床医学院
作者单位1.Minist Educ, Key Lab Assisted Reprod, Beijing 100191, Peoples R China
2.Peking Univ, Hosp 3, Biodynam Opt Imaging Ctr, Beijing 100871, Peoples R China
3.Peking Univ, Dept Obstet & Gynecol, Coll Life Sci, Hosp 3, Beijing 100871, Peoples R China
4.Minist Educ, Key Lab Cell Proliferat & Differentiat, Beijing 100871, Peoples R China
5.Peking Univ, Peking Tsinghua Ctr Life Sci, Beijing 100871, Peoples R China
6.Peking Univ, Coll Chem & Mol Engn, Inst Theoret & Computat Chem, Beijing 100871, Peoples R China
7.Peking Univ, Ctr Mol & Translat Med, Hlth Sci Ctr, Beijing 100191, Peoples R China
8.Beijing Key Lab Reprod Endocrinol & Assisted Repr, Beijing 100191, Peoples R China
9.Peking Univ, Acad Adv Interdisciplinary Studies, Beijing 100871, Peoples R China
推荐引用方式
GB/T 7714
Guo, Fan,Yan, Liying,Guo, Hongshan,et al. The Transcriptome and DNA Methylome Landscapes of Human Primordial Germ Cells[J]. CELL,2015,161(6):1437-1452.
APA Guo, Fan.,Yan, Liying.,Guo, Hongshan.,Li, Lin.,Hu, Boqiang.,...&Qiao, Jie.(2015).The Transcriptome and DNA Methylome Landscapes of Human Primordial Germ Cells.CELL,161(6),1437-1452.
MLA Guo, Fan,et al."The Transcriptome and DNA Methylome Landscapes of Human Primordial Germ Cells".CELL 161.6(2015):1437-1452.
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