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学科主题临床医学
Minocycline delayed photoreceptor death in rds mice through iNOS-dependent mechanism
Yang, Li-ping; Li, Ying; Zhu, Xiu-an; Tso, Mark O. M.
刊名MOLECULAR VISION
2007-07-09
13期:117-18页:1073-1082
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Ophthalmology
研究领域[WOS]Biochemistry & Molecular Biology ; Ophthalmology
关键词[WOS]INDUCED RETINAL DEGENERATION ; NITRIC-OXIDE ; MICROGLIAL ACTIVATION ; MUTANT MICE ; MOUSE MODEL ; CELLS ; EXPRESSION ; RATS ; NEUROPROTECTION ; INFLAMMATION
英文摘要

PURPOSE: To elucidate the role of activated microglia and nitric oxide (NO) in photoreceptor apoptosis in rds mice, and to investigate the effect of minocycline treatment on rds mice.

METHODS: Photoreceptor apoptosis in rds mice was detected by terminal dUTP transferase nick end labeling (TUNEL). Retinal microglial cells were identified by CD11b antibody. The mRNA expression of inducible nitric oxide synthase (iNOS) and chemokines were examined by reverse transcription polymerase chain reaction (RT-PCR) assay. The protein expression of iNOS was examined by immunohistochemistry and Western blotting analysis. The rds mice were treated intra-peritoneally from the second postnatal day (P2) with minocycline.

RESULTS: Accompanying photoreceptor degeneration in rds mice, microglia were activated and immigrated from inner retinal layer (IRL) to outer nuclear layer (ONL), and the expression of iNOS was up-regulated. Minocycline treatment reduced the iNOS expression and decreased the initial photoreceptor apoptosis, but did not provide long term ameliorative effect on the photoreceptor cell loss of rds mice.

CONCLUSIONS: NO played a major role in the initial photoreceptor apoptosis in rds mice. The migration of activated microglia to the ONL contributed to the subsequent photoreceptor cell death; minocycline treatment ameliorated the photoreceptor apoptosis in rds mice, and this protective effect was partly through iNOS-suppressive mechanism.

语种英语
WOS记录号WOS:000248314000001
引用统计
被引频次:24[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/61267
专题北京大学第三临床医学院_眼科
作者单位1.Johns Hopkins Univ, Sch Med, Wilmer Eye Inst, Baltimore, MD 21205 USA
2.Peking Univ, Third Hosp, Ctr Eye, Beijing 100871, Peoples R China
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GB/T 7714
Yang, Li-ping,Li, Ying,Zhu, Xiu-an,et al. Minocycline delayed photoreceptor death in rds mice through iNOS-dependent mechanism[J]. MOLECULAR VISION,2007,13(117-18):1073-1082.
APA Yang, Li-ping,Li, Ying,Zhu, Xiu-an,&Tso, Mark O. M..(2007).Minocycline delayed photoreceptor death in rds mice through iNOS-dependent mechanism.MOLECULAR VISION,13(117-18),1073-1082.
MLA Yang, Li-ping,et al."Minocycline delayed photoreceptor death in rds mice through iNOS-dependent mechanism".MOLECULAR VISION 13.117-18(2007):1073-1082.
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