|Minocycline delayed photoreceptor death in rds mice through iNOS-dependent mechanism|
|Yang, Li-ping; Li, Ying; Zhu, Xiu-an; Tso, Mark O. M.|
|WOS标题词||Science & Technology|
|类目[WOS]||Biochemistry & Molecular Biology ; Ophthalmology|
|研究领域[WOS]||Biochemistry & Molecular Biology ; Ophthalmology|
|关键词[WOS]||INDUCED RETINAL DEGENERATION ; NITRIC-OXIDE ; MICROGLIAL ACTIVATION ; MUTANT MICE ; MOUSE MODEL ; CELLS ; EXPRESSION ; RATS ; NEUROPROTECTION ; INFLAMMATION|
PURPOSE: To elucidate the role of activated microglia and nitric oxide (NO) in photoreceptor apoptosis in rds mice, and to investigate the effect of minocycline treatment on rds mice.
METHODS: Photoreceptor apoptosis in rds mice was detected by terminal dUTP transferase nick end labeling (TUNEL). Retinal microglial cells were identified by CD11b antibody. The mRNA expression of inducible nitric oxide synthase (iNOS) and chemokines were examined by reverse transcription polymerase chain reaction (RT-PCR) assay. The protein expression of iNOS was examined by immunohistochemistry and Western blotting analysis. The rds mice were treated intra-peritoneally from the second postnatal day (P2) with minocycline.
RESULTS: Accompanying photoreceptor degeneration in rds mice, microglia were activated and immigrated from inner retinal layer (IRL) to outer nuclear layer (ONL), and the expression of iNOS was up-regulated. Minocycline treatment reduced the iNOS expression and decreased the initial photoreceptor apoptosis, but did not provide long term ameliorative effect on the photoreceptor cell loss of rds mice.
CONCLUSIONS: NO played a major role in the initial photoreceptor apoptosis in rds mice. The migration of activated microglia to the ONL contributed to the subsequent photoreceptor cell death; minocycline treatment ameliorated the photoreceptor apoptosis in rds mice, and this protective effect was partly through iNOS-suppressive mechanism.
|作者单位||1.Johns Hopkins Univ, Sch Med, Wilmer Eye Inst, Baltimore, MD 21205 USA|
2.Peking Univ, Third Hosp, Ctr Eye, Beijing 100871, Peoples R China
|Yang, Li-ping,Li, Ying,Zhu, Xiu-an,et al. Minocycline delayed photoreceptor death in rds mice through iNOS-dependent mechanism[J]. MOLECULAR VISION,2007,13(117-18):1073-1082.|
|APA||Yang, Li-ping,Li, Ying,Zhu, Xiu-an,&Tso, Mark O. M..(2007).Minocycline delayed photoreceptor death in rds mice through iNOS-dependent mechanism.MOLECULAR VISION,13(117-18),1073-1082.|
|MLA||Yang, Li-ping,et al."Minocycline delayed photoreceptor death in rds mice through iNOS-dependent mechanism".MOLECULAR VISION 13.117-18(2007):1073-1082.|