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学科主题基础医学
Nanopolymers Delivery of the Bone Morphogenetic Protein-4 Plasmid to Mesenchymal Stem Cells Promotes Articular Cartilage Repair In Vitro and In Vivo
Shi, Junjun2; Zhang, Xin2; Pi, Yanbin2; Zhu, Jingxian2; Zhou, Chunyan1; Ao, Yingfang2
刊名JOURNAL OF NANOMATERIALS
2012
DOI10.1155/2012/236953
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary
研究领域[WOS]Science & Technology - Other Topics ; Materials Science
关键词[WOS]GROWTH-FACTOR BETA(1) ; GENE DELIVERY ; TRANSFECTION EFFICIENCY ; TISSUE FORMATION ; DIFFERENTIATION ; VECTORS ; ACTIVATION ; EXPRESSION ; THERAPY ; CULTURE
英文摘要

The clinical application of viral vectors for gene therapy is limited for biosafety consideration. In this study, to promote articular cartilage repair, poly (lactic-co glycolic acid) (PLGA) nanopolymers were used as non-viral vectors to transfect rabbit mesenchymal stem cells (MSCs) with the pDC316-BMP4-EGFP plasmid. The cytotoxicity and transfection efficiency in vitro were acceptable measuring by CCK-8 and flow cytometry. After transfection, Chondrogenic markers (mRNA of Col2a1, Sox9, Bmp4, and Agg) of experimental cells (MSCs being transfected with BMP-4 plasmid by PLGA nanopolymers) were increased more than those of control cells (MSCs being transfected with naked BMP-4 plasmid alone). In vivo study, twelve rabbits (24 knees) with large full thickness articular cartilage defects were randomly divided into the experimental group (MSCs being transfected with BMP-4 plasmid by PLGA nanopolymers) and the control group (MSCs being transfected with naked BMP-4 plasmid). The experimental group showed better regeneration than the control group 6 and 12 weeks postoperatively. Hyaline-like cartilage formed at week 12 in the experimental group, indicating the local delivery of BMP-4 plasmid to MSCs by PLGA nanopolymers improved articular cartilage repair significantly. PLGA nanopolymers could be a promising and effective non-viral vector for gene therapy in cartilage repair.

语种英语
WOS记录号WOS:000304965600001
项目编号2007BAI04B09 ; BMU2009129-112 ; 20100001110086
资助机构National Science and Technology Ministry of China ; Program for Changjiang Scholars and Innovative Research Team in University ; Specialized Research Fund for the Doctoral Program of Higher Education
引用统计
被引频次:2[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/61271
专题北京大学基础医学院_心血管所
北京大学基础医学院
北京大学第三临床医学院_骨科
北京大学第三临床医学院_运动医学研究所
作者单位1.Peking Univ, Minist Educ China, Key Lab Mol Cardiovasc Sci, Dept Biochem & Mol Biol,Sch Basic Med Sci, Beijing 100191, Peoples R China
2.Peking Univ, Hosp 3, Inst Sports Med, Beijing 100191, Peoples R China
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GB/T 7714
Shi, Junjun,Zhang, Xin,Pi, Yanbin,et al. Nanopolymers Delivery of the Bone Morphogenetic Protein-4 Plasmid to Mesenchymal Stem Cells Promotes Articular Cartilage Repair In Vitro and In Vivo[J]. JOURNAL OF NANOMATERIALS,2012.
APA Shi, Junjun,Zhang, Xin,Pi, Yanbin,Zhu, Jingxian,Zhou, Chunyan,&Ao, Yingfang.(2012).Nanopolymers Delivery of the Bone Morphogenetic Protein-4 Plasmid to Mesenchymal Stem Cells Promotes Articular Cartilage Repair In Vitro and In Vivo.JOURNAL OF NANOMATERIALS.
MLA Shi, Junjun,et al."Nanopolymers Delivery of the Bone Morphogenetic Protein-4 Plasmid to Mesenchymal Stem Cells Promotes Articular Cartilage Repair In Vitro and In Vivo".JOURNAL OF NANOMATERIALS (2012).
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