IR@PKUHSC  > 北京大学基础医学院  > 神经生物学系
学科主题基础医学
Identification of a mouse synaptic glycoprotein gene in cultured neurons
Yu, ACH; Sun, CX; Li, Q; Liu, HD; Wang, CR; Zhao, GP; Jin, ML; Lau, LT; Fung, YWW; Liu, SA
关键词primary culture cerebral cortical neurons synaptic glycoprotein bioinformatics
刊名NEUROCHEMICAL RESEARCH
2005-10-01
DOI10.1007/s11064-005-8800-5
30期:10页:1289-1294
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Neurosciences
研究领域[WOS]Biochemistry & Molecular Biology ; Neurosciences & Neurology
关键词[WOS]EXTRACELLULAR-MATRIX GLYCOPROTEIN ; CEREBRAL CORTICAL-NEURONS ; MESSENGER-RNA ; DIFFERENTIAL DISPLAY ; MOLECULAR-CLONING ; TRANSCRIPTS ; ASTROCYTES ; GLUTAMATE ; SC1
英文摘要

Neuronal differentiation and aging are known to involve many genes, which may also be differentially expressed during these developmental processes. From primary cultured cerebral cortical neurons, we have previously identified various differentially expressed gene transcripts from cultured cortical neurons using the technique of arbitrarily primed PCR (RAP-PCR). Among these transcripts, clone 0-2 was found to have high homology to rat and human synaptic glycoprotein. By in silico analysis using an EST database and the FACTURA software, the full-length sequence of 0-2 was assembled and the clone was named as mouse synaptic glycoprotein homolog 2 (mSC2). DNA sequencing revealed transcript size of mSC2 being smaller than the human and rat homologs. RT-PCR indicated that mSC2 was expressed differentially at various culture days. The mSC2 gene was located in various tissues with higher expression in brain, lung, and liver. Functions of mSC2 in neurons and other tissues remain elusive and will require more investigation.

语种英语
WOS记录号WOS:000233866800010
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Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/61274
Collection北京大学基础医学院_神经生物学系
作者单位1.Peking Univ, Neurosci Res Inst, Beijing 100871, Peoples R China
2.Minist Educ Res, Key Lab Neurosci, Beijing, Peoples R China
3.Peking Univ, Dept Neurobiol, Hlth Sci Ctr, Beijing 100871, Peoples R China
4.Chinese Acad Sci, Shanghai Brain Res Inst, Shanghai Res Ctr Life Sci, Shanghai, Peoples R China
5.Hong Kong DNA Chips Ltd, Hong Kong, Hong Kong, Peoples R China
6.Chinese Acad Sci, Shanghai Res Ctr Biotechnol, Shanghai Inst Biol Sci, Shanghai, Peoples R China
Recommended Citation
GB/T 7714
Yu, ACH,Sun, CX,Li, Q,et al. Identification of a mouse synaptic glycoprotein gene in cultured neurons[J]. NEUROCHEMICAL RESEARCH,2005,30(10):1289-1294.
APA Yu, ACH.,Sun, CX.,Li, Q.,Liu, HD.,Wang, CR.,...&Liu, SA.(2005).Identification of a mouse synaptic glycoprotein gene in cultured neurons.NEUROCHEMICAL RESEARCH,30(10),1289-1294.
MLA Yu, ACH,et al."Identification of a mouse synaptic glycoprotein gene in cultured neurons".NEUROCHEMICAL RESEARCH 30.10(2005):1289-1294.
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