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Synthesis of unimolecularly circular G-quadruplexes as prospective molecular probes
Zhou, TY; Chen, GS; Wang, YF; Zhang, Q; Yang, M; Li, TH
刊名NUCLEIC ACIDS RESEARCH
2004
DOI10.1093/nar/gnh162
32期:21
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology
研究领域[WOS]Biochemistry & Molecular Biology
关键词[WOS]TELOMERE-BINDING-PROTEIN ; SINGLE-STRANDED-DNA ; G-QUARTET FORMATION ; TETRAPLEX FORMATION ; CRYSTAL-STRUCTURE ; C-MYC ; BETA-SUBUNIT ; OLIGONUCLEOTIDES ; APTAMER ; THERMODYNAMICS
英文摘要

Synthesis of unimolecularly circular G-quadruplex has been accomplished for the first time during our investigation on the template basis of G-quadruplex through chemical ligations of guanine-rich linear sequences of oligodeoxyribonucleotides. The uniqueness of this newly designed circularization course is its self-recognition and self-templating on the scale of individual strand of oligodeoxyribonucleotide in which the same linear sequence serves both as a template and as a substrate simultaneously. The results from our exonuclease and DNAse hydrolysis studies confirm that there is indeed absence of open termini within the structure of the identified circular product. Our subsequent investigation on the loop-size effect indicates that the unimolecularly circular G-quadruplex possessing two or more thymine nucleotides within their connecting loops is readily attainable, while the linear sequence with a single thymine nucleotide between guanine tracts is not a proper precursor for our ligation reaction. In addition, conformation dependency of the circularization course as well as the effects of alkali ions, pH values and concentration of potassium ions on the circularization reaction are examined during our investigation. The implication of our current studies and possible application of the obtained unimolecularly circular G-quadruplex in certain biological processes are also discussed in this report.

语种英语
WOS记录号WOS:000225676700008
Citation statistics
Cited Times:13[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/61295
Collection北京大学药学院_药剂学系
作者单位1.Natl Univ Singapore, Dept Chem, Singapore 117543, Singapore
2.Peking Univ, Sch Pharm, Dept Pharmaceut, Beijing 100083, Peoples R China
3.Peking Univ, Natl Res Labs & Biomimet Drugs, Beijing 100083, Peoples R China
4.Hong Kong Polytech Univ, Dept Appl Biol & Chem Technol, Kowloon, Hong Kong, Peoples R China
Recommended Citation
GB/T 7714
Zhou, TY,Chen, GS,Wang, YF,et al. Synthesis of unimolecularly circular G-quadruplexes as prospective molecular probes[J]. NUCLEIC ACIDS RESEARCH,2004,32(21).
APA Zhou, TY,Chen, GS,Wang, YF,Zhang, Q,Yang, M,&Li, TH.(2004).Synthesis of unimolecularly circular G-quadruplexes as prospective molecular probes.NUCLEIC ACIDS RESEARCH,32(21).
MLA Zhou, TY,et al."Synthesis of unimolecularly circular G-quadruplexes as prospective molecular probes".NUCLEIC ACIDS RESEARCH 32.21(2004).
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