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In vitro genotoxicity of danthron and its potential mechanism
Zhang, Zhaohui; Fu, Juanling; Yao, Biyun; Zhang, Xiaolin; Zhao, Peng; Zhou, Zongcan
关键词Danthron 1,8-Dihydroxyanthraquinone Mutagenicity DT-diaphorase Oxidative stress
刊名MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS
2011-05-18
DOI10.1016/j.mrgentox.2011.02.006
722期:1页:39-43
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biotechnology & Applied Microbiology ; Genetics & Heredity ; Toxicology
研究领域[WOS]Biotechnology & Applied Microbiology ; Genetics & Heredity ; Toxicology
关键词[WOS]TOPOISOMERASE-II ; MAMMALIAN-CELLS ; ANTHRAQUINONES ; LIVER ; MUTAGENICITY ; TOXICOLOGY ; STRAINS ; TA102 ; ASSAY
英文摘要

To ascertain the in vitro genotoxicity of danthron and its potential mechanism of action, we performed an Ames test, a cytokinesis-block micronucleus assay and a comet assay in Balb/c 3T3 cells. The Ames test revealed that danthron was mutagenic only toward Salmonella typhimurium strain TA102 in the presence of an exogenous metabolic activation system (S9 mix). Danthron (25,50 and 10011 mu g/ml) increased the frequencies of micronuclear cells with or without S9 mix, and the comet length, tail length and Olive tail moment in comet assays without S9 mix in a dose-dependent manner. These results demonstrated the in vitro genotoxicity of danthron and that 3T3 cells are capable of activating danthron. When NADP was replaced by MAD in the S9 mix, danthron remained mutagenic toward strain TA102. The addition of dicoumarol, a DT-diaphorase inhibitor, decreased the number of danthron-induced histidine revertants by 35-39%, indicating that DT-diaphorase is involved in the metabolic activation of danthron in the presence of NADH as an electron donor. In 3T3 cells, increases in reactive oxygen species (ROS) formation and 8-hydroxydeoxyguanosine levels as well as a reduction in GSH levels were induced by danthron in a dose-dependent manner, indicating that oxidative stress may be a major contributing pathway in the genotoxicity of danthron. (C) 2011 Elsevier B.V. All rights reserved.

语种英语
WOS记录号WOS:000291913600005
引用统计
被引频次:6[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/61297
专题北京大学公共卫生学院_毒理学系
北京大学公共卫生学院_公共卫生学院
北京大学第三临床医学院_肿瘤治疗中心
北京大学精神卫生研究所_财务处
作者单位Peking Univ, Dept Toxicol, Hlth Sci Ctr, Beijing 100191, Peoples R China
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GB/T 7714
Zhang, Zhaohui,Fu, Juanling,Yao, Biyun,et al. In vitro genotoxicity of danthron and its potential mechanism[J]. MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS,2011,722(1):39-43.
APA Zhang, Zhaohui,Fu, Juanling,Yao, Biyun,Zhang, Xiaolin,Zhao, Peng,&Zhou, Zongcan.(2011).In vitro genotoxicity of danthron and its potential mechanism.MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS,722(1),39-43.
MLA Zhang, Zhaohui,et al."In vitro genotoxicity of danthron and its potential mechanism".MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS 722.1(2011):39-43.
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