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Evaluation of the effect of TM208 on the activity of five cytochrome P450 enzymes using on-line solid-phase extraction HPLC-DAD: A cocktail approach
Lin, Wensi1; Zhang, Jianmei1; Ling, Xiaomei1,2; Yu, Ning1; Li, Jing1; Yang, Haisong1; Li, Runtao2; Cui, Jingrong2
关键词Probe drug cocktail Online solid-phase extraction HPLC TM208 Rat plasma
刊名JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
2013-04-01
DOI10.1016/j.jchromb.2013.01.031
923页:29-36
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]TANDEM MASS-SPECTROMETRY ; 3-CYANO-3,3-DIPHENYLPROPYL ESTER HYDROCHLORIDE ; INTERINDIVIDUAL VARIATIONS ; PHENOTYPING PROBES ; MAJOR METABOLITES ; DRUG-METABOLISM ; IN-VIVO ; LC-MS ; P450 ; RAT
英文摘要

A rapid, simple, and sensitive on-line solid-phase extraction HPLC-DAD method for simultaneous evaluation of the activity of five CYP450 isoforms (CYP1A2, CYP2C19, CYP2D6, CYP2E1 and CYP3A4) in vivo has been developed and validated. The five specific probe substrates include caffeine (1A2),metoprolol (2D6), dapsone (3A4), omeprazole (2C19) and chlorzoxazone (2E1). Automated pre-purification of plasma and enrichment of analytes were performed using a C18 on-line solid-phase extraction cartridge. After being eluted from the cartridge, the analytes and the internal standard antipyrine were separated on a C18 RP analytical column and analyzed by DAD. The method was validated to quantify the concentration ranges of 0.05-50.0 mu g/ml for dapsone and omeprazole, 0.1-50.0 mu g/ml for caffeine and 0.2-50.0 mu g/ml for metoprolol and chlorzoxazone. The linearity (R-2) for all analytes tested was exceeded 0.99. The intra-day precision ranged from 0.29 to 13% and the inter-day precision ranged from 5.0 to 15%, respectively. The intra-day and inter-day accuracy were between 86.7% and 113.6%. The extraction recoveries were in the range 82.8-109.9% for all the analytes and internal standard antipyrine. This method was successfully applied to evaluate the effects of TM208 on rat five CYP450 isoforms. (c) 2013 Elsevier B.V. All rights reserved.

语种英语
WOS记录号WOS:000317148400005
项目编号81072612 ; 7102107 ; K20110109 ; 20110001110021
资助机构National Natural Science Foundation ; Natural Science Foundation of Beijing ; Open Foundation of State Key Laboratory of Natural and Biomimetic Drugs ; Specialized Research Fund for the Doctoral Program of Higher Education of China
引用统计
被引频次:10[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/61308
专题北京大学药学院_化学生物学系
北京大学药学院_分子与细胞药理学系
北京大学第一临床医学院_眼科
作者单位1.Peking Univ, Dept Pharmaceut Anal, Sch Pharmaceut Sci, Beijing 100871, Peoples R China
2.Peking Univ, State Key Lab Nat & Biomimet Drugs, Beijing 100871, Peoples R China
推荐引用方式
GB/T 7714
Lin, Wensi,Zhang, Jianmei,Ling, Xiaomei,et al. Evaluation of the effect of TM208 on the activity of five cytochrome P450 enzymes using on-line solid-phase extraction HPLC-DAD: A cocktail approach[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2013,923:29-36.
APA Lin, Wensi.,Zhang, Jianmei.,Ling, Xiaomei.,Yu, Ning.,Li, Jing.,...&Cui, Jingrong.(2013).Evaluation of the effect of TM208 on the activity of five cytochrome P450 enzymes using on-line solid-phase extraction HPLC-DAD: A cocktail approach.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,923,29-36.
MLA Lin, Wensi,et al."Evaluation of the effect of TM208 on the activity of five cytochrome P450 enzymes using on-line solid-phase extraction HPLC-DAD: A cocktail approach".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 923(2013):29-36.
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