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学科主题: 临床医学
题名:
A DNA vaccine targeting p42.3 induces protective antitumor immunity via eliciting cytotoxic CD8(+)T lymphocytes in a murine melanoma model
作者: Liu, Hu1; Geng, Shuang2,3; Feng, Congcong1; Xie, Xiaoping1; Wu, Bin1; Chen, Xuan1; Zou, Qiang1; Wang, Shuang1; Cui, Jiantao4; Xing, Rui4; Li, Wenmei4; Lu, Youyong4; Wang, Bin1,2,3
关键词: cancer immunotherapy ; p42.3 ; DNA vaccine ; cytotoxic CD8(+)T cell ; B16F10 melanoma
刊名: HUMAN VACCINES & IMMUNOTHERAPEUTICS
发表日期: 2013-10-01
DOI: 10.4161/hv.25013
卷: 9, 期:10, 页:2196-2202
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biotechnology & Applied Microbiology ; Immunology
研究领域[WOS]: Biotechnology & Applied Microbiology ; Immunology
关键词[WOS]: T-CELL ; B16F10 MELANOMA ; IN-VIVO ; CANCER ; ELECTROPORATION ; RESPONSES ; EFFICACY ; EXPRESSION ; DELIVERY ; IL-15
英文摘要:

The p42.3 gene was recently identified and characterized as having tumor-specific and mitosis phase-dependent expression in many types of cancer. This suggested that p42.3 antigen could be used as a target for vaccines against cancers. In this study, we immunized C57BL/6 mice with a DNA vaccine encoding p42.3. We used intramuscular injection with electroporation, either before or after challenge with tumor B16F10 cells. Vaccination with pcDNA3-p42.3 induced some degree of antitumor effect both therapeutically and prophylactically, as evaluated by the inhibition of tumor growth and decrease in tumor weight. Immunized mice showed a high level of specific cytotoxic activity against the p42.3 protein in vivo and had activated CD8 T cells that secreted IFN-gamma, perforin, and granzyme B in response to stimulation with the antigen in vitro. Thus, this study presents the DNA vaccination against novel tumor target p42.3 as a promising antitumor modality.

语种: 英语
所属项目编号: 30930068 ; 2010AA022907
项目资助者: Chinese National Natural Science Foundation ; National High-Tech 863 Project of China
WOS记录号: WOS:000330381600027
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/61325
Appears in Collections:北京大学临床肿瘤学院_分子肿瘤学研究室_期刊论文

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作者单位: 1.China Agr Univ, State Key Lab Agrobiotechnol, Coll Biol Sci, Beijing 100094, Peoples R China
2.Fudan Univ, Key Lab Med Mol Virol, MOH, Shanghai Med Coll, Shanghai 200433, Peoples R China
3.Fudan Univ, Shanghai Med Coll, MOE, Shanghai 200433, Peoples R China
4.Peking Univ, Lab Mol Oncol, Key Lab Carcinogenesis & Translat Res, Canc Hosp & Inst,Minist Educ, Beijing 100871, Peoples R China

Recommended Citation:
Liu, Hu,Geng, Shuang,Feng, Congcong,et al. A DNA vaccine targeting p42.3 induces protective antitumor immunity via eliciting cytotoxic CD8(+)T lymphocytes in a murine melanoma model[J]. HUMAN VACCINES & IMMUNOTHERAPEUTICS,2013,9(10):2196-2202.
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