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学科主题临床医学
A DNA vaccine targeting p42.3 induces protective antitumor immunity via eliciting cytotoxic CD8(+)T lymphocytes in a murine melanoma model
Liu, Hu1; Geng, Shuang2,3; Feng, Congcong1; Xie, Xiaoping1; Wu, Bin1; Chen, Xuan1; Zou, Qiang1; Wang, Shuang1; Cui, Jiantao4; Xing, Rui4; Li, Wenmei4; Lu, Youyong4; Wang, Bin1,2,3
关键词cancer immunotherapy p42.3 DNA vaccine cytotoxic CD8(+)T cell B16F10 melanoma
刊名HUMAN VACCINES & IMMUNOTHERAPEUTICS
2013-10-01
DOI10.4161/hv.25013
9期:10页:2196-2202
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biotechnology & Applied Microbiology ; Immunology
研究领域[WOS]Biotechnology & Applied Microbiology ; Immunology
关键词[WOS]T-CELL ; B16F10 MELANOMA ; IN-VIVO ; CANCER ; ELECTROPORATION ; RESPONSES ; EFFICACY ; EXPRESSION ; DELIVERY ; IL-15
英文摘要

The p42.3 gene was recently identified and characterized as having tumor-specific and mitosis phase-dependent expression in many types of cancer. This suggested that p42.3 antigen could be used as a target for vaccines against cancers. In this study, we immunized C57BL/6 mice with a DNA vaccine encoding p42.3. We used intramuscular injection with electroporation, either before or after challenge with tumor B16F10 cells. Vaccination with pcDNA3-p42.3 induced some degree of antitumor effect both therapeutically and prophylactically, as evaluated by the inhibition of tumor growth and decrease in tumor weight. Immunized mice showed a high level of specific cytotoxic activity against the p42.3 protein in vivo and had activated CD8 T cells that secreted IFN-gamma, perforin, and granzyme B in response to stimulation with the antigen in vitro. Thus, this study presents the DNA vaccination against novel tumor target p42.3 as a promising antitumor modality.

语种英语
WOS记录号WOS:000330381600027
项目编号30930068 ; 2010AA022907
资助机构Chinese National Natural Science Foundation ; National High-Tech 863 Project of China
引用统计
被引频次:8[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/61325
专题北京大学临床肿瘤学院_分子肿瘤学研究室
北京大学临床肿瘤学院_高技术实验室
作者单位1.China Agr Univ, State Key Lab Agrobiotechnol, Coll Biol Sci, Beijing 100094, Peoples R China
2.Fudan Univ, Key Lab Med Mol Virol, MOH, Shanghai Med Coll, Shanghai 200433, Peoples R China
3.Fudan Univ, Shanghai Med Coll, MOE, Shanghai 200433, Peoples R China
4.Peking Univ, Lab Mol Oncol, Key Lab Carcinogenesis & Translat Res, Canc Hosp & Inst,Minist Educ, Beijing 100871, Peoples R China
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Liu, Hu,Geng, Shuang,Feng, Congcong,et al. A DNA vaccine targeting p42.3 induces protective antitumor immunity via eliciting cytotoxic CD8(+)T lymphocytes in a murine melanoma model[J]. HUMAN VACCINES & IMMUNOTHERAPEUTICS,2013,9(10):2196-2202.
APA Liu, Hu.,Geng, Shuang.,Feng, Congcong.,Xie, Xiaoping.,Wu, Bin.,...&Wang, Bin.(2013).A DNA vaccine targeting p42.3 induces protective antitumor immunity via eliciting cytotoxic CD8(+)T lymphocytes in a murine melanoma model.HUMAN VACCINES & IMMUNOTHERAPEUTICS,9(10),2196-2202.
MLA Liu, Hu,et al."A DNA vaccine targeting p42.3 induces protective antitumor immunity via eliciting cytotoxic CD8(+)T lymphocytes in a murine melanoma model".HUMAN VACCINES & IMMUNOTHERAPEUTICS 9.10(2013):2196-2202.
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