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学科主题: 临床医学
题名:
Derivation, culture and retinal pigment epithelial differentiation of human embryonic stem cells using human fibroblast feeder cells
作者: Zhang, Yun-Shan1; Lu, Zhen-Yu1,2; Yu, Yang3; Li, Xiao-Rong4; Li, Wen-Bo4; Wang, Yi-Na1; Geng, Ying1
关键词: Human embryonic stem cell ; Human foreskin fibroblast feeder layer ; Human abdominal fibroblast feeder layer ; Retinal pigment epithelium differentiation
刊名: JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
发表日期: 2012-08-01
DOI: 10.1007/s10815-012-9802-2
卷: 29, 期:8, 页:735-744
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Genetics & Heredity ; Obstetrics & Gynecology ; Reproductive Biology
研究领域[WOS]: Genetics & Heredity ; Obstetrics & Gynecology ; Reproductive Biology
关键词[WOS]: PROLONGED UNDIFFERENTIATED GROWTH ; MACULAR DEGENERATION ; VISUAL FUNCTION ; IN-VITRO ; GENERATION ; LINES ; MOUSE
英文摘要:

Retinal pigment epithelium cells derived from human embryonic stem cells (ESCs) could be useful for restoring retinal function in age-related macular degeneration. However the use of non-human feeder cells to support the growth of ESCs for clinical applications raises the concern of possible contamination because of direct contact between animal and human cells.

In this study, we produced human ESCs using human fibroblast feeder layers isolated from foreskin and abdominal tissues. Using this system, human ESCs differentiated into retinal pigment epithelium cells in differentiation medium.

Seven human ESC lines were established from 18 blastocysts. These human ESCs showed normal morphology, expressed all expected cell surface markers, had the ability to form embryoid bodies upon culture in vitro and teratomas after injection into SCID mice, and differentiated further into derivatives of all three germ layers. Under conditions of committed differentiation, these human ESCs could differentiate into retinal pigment epithelium cells after 2 months in culture.

The results of this study demonstrated that human foreskin/abdominal fibroblasts have the potential to support the derivation and long-term culture of human ESCs, which can then be used to generate retinal pigment epithelium cells with characteristic morphology and molecular markers. This technique avoids the concerns of contamination from animal feeder layers during human ESC derivation, culture and differentiation, and will thus facilitate the development of retinal pigment epithelium cell transplantation therapy.

语种: 英语
所属项目编号: 81100404 ; 30973255 ; 20110001120008
项目资助者: National Natural Science Foundation of China of Young Scholars ; National Natural Science Foundation of China ; Specialized Research Fund for the Doctoral Program of Higher Education of China
WOS记录号: WOS:000308184900005
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/61331
Appears in Collections:北京大学第三临床医学院_生殖医学中心_期刊论文

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作者单位: 1.Union Stem Cell & Gene Engn Co Ltd, Tianjin 300384, Peoples R China
2.Tianjin Med Univ, Ctr Eye, Tianjin 300384, Peoples R China
3.Tianjin Cent Hosp Obstet & Gynecol, Ctr Reprod Med, Tianjin 300100, Peoples R China
4.Peking Univ, Hosp 3, Dept Obstet & Gynecol, Ctr Reprod Med, Beijing 100191, Peoples R China

Recommended Citation:
Zhang, Yun-Shan,Lu, Zhen-Yu,Yu, Yang,et al. Derivation, culture and retinal pigment epithelial differentiation of human embryonic stem cells using human fibroblast feeder cells[J]. JOURNAL OF ASSISTED REPRODUCTION AND GENETICS,2012,29(8):735-744.
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