|Derivation, culture and retinal pigment epithelial differentiation of human embryonic stem cells using human fibroblast feeder cells|
|Zhang, Yun-Shan1; Lu, Zhen-Yu1,2; Yu, Yang3; Li, Xiao-Rong4; Li, Wen-Bo4; Wang, Yi-Na1; Geng, Ying1|
|关键词||Human embryonic stem cell Human foreskin fibroblast feeder layer Human abdominal fibroblast feeder layer Retinal pigment epithelium differentiation|
|刊名||JOURNAL OF ASSISTED REPRODUCTION AND GENETICS|
|WOS标题词||Science & Technology|
|类目[WOS]||Genetics & Heredity ; Obstetrics & Gynecology ; Reproductive Biology|
|研究领域[WOS]||Genetics & Heredity ; Obstetrics & Gynecology ; Reproductive Biology|
|关键词[WOS]||PROLONGED UNDIFFERENTIATED GROWTH ; MACULAR DEGENERATION ; VISUAL FUNCTION ; IN-VITRO ; GENERATION ; LINES ; MOUSE|
Retinal pigment epithelium cells derived from human embryonic stem cells (ESCs) could be useful for restoring retinal function in age-related macular degeneration. However the use of non-human feeder cells to support the growth of ESCs for clinical applications raises the concern of possible contamination because of direct contact between animal and human cells.
In this study, we produced human ESCs using human fibroblast feeder layers isolated from foreskin and abdominal tissues. Using this system, human ESCs differentiated into retinal pigment epithelium cells in differentiation medium.
Seven human ESC lines were established from 18 blastocysts. These human ESCs showed normal morphology, expressed all expected cell surface markers, had the ability to form embryoid bodies upon culture in vitro and teratomas after injection into SCID mice, and differentiated further into derivatives of all three germ layers. Under conditions of committed differentiation, these human ESCs could differentiate into retinal pigment epithelium cells after 2 months in culture.
The results of this study demonstrated that human foreskin/abdominal fibroblasts have the potential to support the derivation and long-term culture of human ESCs, which can then be used to generate retinal pigment epithelium cells with characteristic morphology and molecular markers. This technique avoids the concerns of contamination from animal feeder layers during human ESC derivation, culture and differentiation, and will thus facilitate the development of retinal pigment epithelium cell transplantation therapy.
|项目编号||81100404 ; 30973255 ; 20110001120008|
|资助机构||National Natural Science Foundation of China of Young Scholars ; National Natural Science Foundation of China ; Specialized Research Fund for the Doctoral Program of Higher Education of China|
|作者单位||1.Union Stem Cell & Gene Engn Co Ltd, Tianjin 300384, Peoples R China|
2.Tianjin Med Univ, Ctr Eye, Tianjin 300384, Peoples R China
3.Tianjin Cent Hosp Obstet & Gynecol, Ctr Reprod Med, Tianjin 300100, Peoples R China
4.Peking Univ, Hosp 3, Dept Obstet & Gynecol, Ctr Reprod Med, Beijing 100191, Peoples R China
|Zhang, Yun-Shan,Lu, Zhen-Yu,Yu, Yang,et al. Derivation, culture and retinal pigment epithelial differentiation of human embryonic stem cells using human fibroblast feeder cells[J]. JOURNAL OF ASSISTED REPRODUCTION AND GENETICS,2012,29(8):735-744.|
|APA||Zhang, Yun-Shan.,Lu, Zhen-Yu.,Yu, Yang.,Li, Xiao-Rong.,Li, Wen-Bo.,...&Geng, Ying.(2012).Derivation, culture and retinal pigment epithelial differentiation of human embryonic stem cells using human fibroblast feeder cells.JOURNAL OF ASSISTED REPRODUCTION AND GENETICS,29(8),735-744.|
|MLA||Zhang, Yun-Shan,et al."Derivation, culture and retinal pigment epithelial differentiation of human embryonic stem cells using human fibroblast feeder cells".JOURNAL OF ASSISTED REPRODUCTION AND GENETICS 29.8(2012):735-744.|