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学科主题: 临床医学
题名:
The novel focal adhesion gene kindlin-2 promotes the invasion of gastric cancer cells mediated by tumor-associated macrophages
作者: Shen, Zhanlong1,2,3; Ye, Yingjiang1; Kauttu, Tuuli2,3; Seppanen, Hanna2,3; Vainionpaa, Sanna2,3; Wang, Shan1; Mustonen, Harri2,3; Puolakkainen, Pauli2,3
关键词: kindlin-2 ; tumor-associated macrophages ; interleukin ; gastric cancer ; invasion
刊名: ONCOLOGY REPORTS
发表日期: 2013-02-01
DOI: 10.3892/or.2012.2137
卷: 29, 期:2, 页:791-797
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology
研究领域[WOS]: Oncology
关键词[WOS]: MATRIX ADHESION ; BLADDER-CANCER ; FAS LIGAND ; INTERLEUKIN-18 ; EXPRESSION ; CARCINOMA ; PROGRESSION ; ANGIOGENESIS ; LOCALIZATION ; INFILTRATION
英文摘要:

Kindlin-2 is a novel focal adhesion gene mediating the cell-extracellular matrix (ECM) adhesion. Tumor-associated macrophages (TAMs) play an important role in linking chronic inflammation to cancer progression. Both kindlin-2 and TAMs have been found to promote the invasion of gastric cancer cells in our previous studies. However, the correlation between kindlin-2 and TAMs remains unclear. Real-time RT-PCR was used to investigate kindlin-2 expression in the AGS, NCI and Hs-746T gastric cancer cell lines co-cultured with TAMs under normal or hypoxic conditions. IL8, IL10, IL11, IL17b, IL18, IL22 and IL24 expressions were measured by real-time RT-PCR in the gastric cancer lines with varying levels of kindlin-2 expression, as well as after downregulation of kindlin-2 mRNA expression by the siRNA method. We found that kindlin-2 was upregulated in all three gastric cancer cell lines when co-cultured with TAMs under normal conditions. Under hypoxic conditions, the induction of kindlin-2 expression induced by macrophages was significantly down-regulated in the Hs-746T cell line. IL8, IL11, IL17b, IL22 and IL24 expression was significantly higher in gastric cell lines with high kindlin-2 expression. Downregulation of kindlin-2 mRNA decreased IL10, IL11, IL17b, IL22 and IL24 expression but IL8 and IL18 expression was upregulated. Therefore, the novel focal adhesion gene kindlin-2 may play an important role in promoting the invasion of gastric cancer cells mediated by TAMs through regulating interleukin expression.

语种: 英语
项目资助者: Sigrid Juselius Foundation ; Helsinki University Central Hospital Research Funds
WOS记录号: WOS:000313605100052
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/61337
Appears in Collections:北京大学第二临床医学院_胃肠外科_期刊论文

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作者单位: 1.Univ Helsinki, FIN-00290 Helsinki, Finland
2.Peking Univ, Dept Surg Gastroenterol, Peoples Hosp, Beijing 100044, Peoples R China
3.Univ Helsinki, Cent Hosp, Dept Surg, FIN-00290 Helsinki, Finland

Recommended Citation:
Shen, Zhanlong,Ye, Yingjiang,Kauttu, Tuuli,et al. The novel focal adhesion gene kindlin-2 promotes the invasion of gastric cancer cells mediated by tumor-associated macrophages[J]. ONCOLOGY REPORTS,2013,29(2):791-797.
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