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学科主题临床医学
The novel focal adhesion gene kindlin-2 promotes the invasion of gastric cancer cells mediated by tumor-associated macrophages
Shen, Zhanlong1,2,3; Ye, Yingjiang1; Kauttu, Tuuli2,3; Seppanen, Hanna2,3; Vainionpaa, Sanna2,3; Wang, Shan1; Mustonen, Harri2,3; Puolakkainen, Pauli2,3
关键词kindlin-2 tumor-associated macrophages interleukin gastric cancer invasion
刊名ONCOLOGY REPORTS
2013-02-01
DOI10.3892/or.2012.2137
29期:2页:791-797
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Oncology
研究领域[WOS]Oncology
关键词[WOS]MATRIX ADHESION ; BLADDER-CANCER ; FAS LIGAND ; INTERLEUKIN-18 ; EXPRESSION ; CARCINOMA ; PROGRESSION ; ANGIOGENESIS ; LOCALIZATION ; INFILTRATION
英文摘要

Kindlin-2 is a novel focal adhesion gene mediating the cell-extracellular matrix (ECM) adhesion. Tumor-associated macrophages (TAMs) play an important role in linking chronic inflammation to cancer progression. Both kindlin-2 and TAMs have been found to promote the invasion of gastric cancer cells in our previous studies. However, the correlation between kindlin-2 and TAMs remains unclear. Real-time RT-PCR was used to investigate kindlin-2 expression in the AGS, NCI and Hs-746T gastric cancer cell lines co-cultured with TAMs under normal or hypoxic conditions. IL8, IL10, IL11, IL17b, IL18, IL22 and IL24 expressions were measured by real-time RT-PCR in the gastric cancer lines with varying levels of kindlin-2 expression, as well as after downregulation of kindlin-2 mRNA expression by the siRNA method. We found that kindlin-2 was upregulated in all three gastric cancer cell lines when co-cultured with TAMs under normal conditions. Under hypoxic conditions, the induction of kindlin-2 expression induced by macrophages was significantly down-regulated in the Hs-746T cell line. IL8, IL11, IL17b, IL22 and IL24 expression was significantly higher in gastric cell lines with high kindlin-2 expression. Downregulation of kindlin-2 mRNA decreased IL10, IL11, IL17b, IL22 and IL24 expression but IL8 and IL18 expression was upregulated. Therefore, the novel focal adhesion gene kindlin-2 may play an important role in promoting the invasion of gastric cancer cells mediated by TAMs through regulating interleukin expression.

语种英语
WOS记录号WOS:000313605100052
资助机构Sigrid Juselius Foundation ; Helsinki University Central Hospital Research Funds
引用统计
被引频次:11[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/61337
专题北京大学第二临床医学院_胃肠外科
作者单位1.Univ Helsinki, FIN-00290 Helsinki, Finland
2.Peking Univ, Dept Surg Gastroenterol, Peoples Hosp, Beijing 100044, Peoples R China
3.Univ Helsinki, Cent Hosp, Dept Surg, FIN-00290 Helsinki, Finland
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Shen, Zhanlong,Ye, Yingjiang,Kauttu, Tuuli,et al. The novel focal adhesion gene kindlin-2 promotes the invasion of gastric cancer cells mediated by tumor-associated macrophages[J]. ONCOLOGY REPORTS,2013,29(2):791-797.
APA Shen, Zhanlong.,Ye, Yingjiang.,Kauttu, Tuuli.,Seppanen, Hanna.,Vainionpaa, Sanna.,...&Puolakkainen, Pauli.(2013).The novel focal adhesion gene kindlin-2 promotes the invasion of gastric cancer cells mediated by tumor-associated macrophages.ONCOLOGY REPORTS,29(2),791-797.
MLA Shen, Zhanlong,et al."The novel focal adhesion gene kindlin-2 promotes the invasion of gastric cancer cells mediated by tumor-associated macrophages".ONCOLOGY REPORTS 29.2(2013):791-797.
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