IR@PKUHSC  > 北京大学深圳医院  > 泌尿外科
学科主题临床医学
PGAM1 is Involved in Spermatogenic Dysfunction and Affects Cell Proliferation, Apoptosis, and Migration
Zhang, Shoubo1,2; Zhao, Yuanshu3; Lei, Bin1; Li, Chunjing1,4; Mao, Xiangming1,5
关键词PGAM1 male infertility biological functions GC1 TM4
刊名REPRODUCTIVE SCIENCES
2015-10-01
DOI10.1177/1933719115572485
22期:10页:1236-1242
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Obstetrics & Gynecology ; Reproductive Biology
资助者National Natural Science Foundation of China ; National Natural Science Foundation of China
研究领域[WOS]Obstetrics & Gynecology ; Reproductive Biology
关键词[WOS]PHOSPHOGLYCERATE MUTASE 1 ; MALE-FERTILITY ; P53 ; CARCINOMA ; HYPOXIA ; IDENTIFICATION ; BIOSYNTHESIS ; GLYCOLYSIS ; ENZYMES ; KINASE
英文摘要

Objective: To investigate the correlation between PGAM1 and spermatogenic dysfunction and to evaluate the effect of expression of PGAM1 on the function of germ cells.

Methods: Expression of PGAM1 was detected in 40 cases of infertile males with definite pathological diagnosis and 12 cases of mouse models with spermatogenic dysfunction by immunohistochemistry. Then, cell proliferation, apoptosis, and migration were evaluated when expression of PGAM1 was knocked down by a specific small interfering RNA in GC1 and TM4 cells.

Results: The positive rates of PGAM1 in patients with normal spermatogenesis, mild hypospermatogenesis, severe hypospermatogenesis, and Sertoli cell-only syndrome were 90%, 80%, 10%, 100%, respectively, and the difference was significant (P < .001). Meanwhile, expression of PGAM1 was found to be significantly decreased in mouse models with spermatogenic dysfunction. Moreover, when expression of PGAM1 was knocked down in GC1 cells, the proliferation and migration were significantly inhibited, but the rate of apoptosis was significantly increased. Furthermore, PGAM1 downregulation in TM4 cells significantly inhibited proliferation and promoted apoptosis but didn′t affect migration.

Conclusion: PGAM1 correlates with spermatogenic distinction and affects the function of cell proliferation, apoptosis and migration.

语种英语
所属项目编号81270743
资助者National Natural Science Foundation of China ; National Natural Science Foundation of China
WOS记录号WOS:000361530300007
Citation statistics
Cited Times:2[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/61360
Collection北京大学深圳医院_泌尿外科
作者单位1.Southern Med Univ, Nanfang Hosp, Dept Urol, Guangzhou 510515, Guangdong, Peoples R China
2.Guangzhou Med Univ, Guangdong Armed Police Hosp, Dept Obstet & Gynecol, Ctr Reprod Med, Guangzhou, Guangdong, Peoples R China
3.Guangzhou Med Univ, Funct Expt Ctr, Guangzhou, Guangdong, Peoples R China
4.Women & Childrens Hosp Foshan, Dept Surg, Foshan, Guangdong, Peoples R China
5.Peking Univ, Shenzhen Hosp, Dept Urol, Shenzhen, Guangdong, Peoples R China
Recommended Citation
GB/T 7714
Zhang, Shoubo,Zhao, Yuanshu,Lei, Bin,et al. PGAM1 is Involved in Spermatogenic Dysfunction and Affects Cell Proliferation, Apoptosis, and Migration[J]. REPRODUCTIVE SCIENCES,2015,22(10):1236-1242.
APA Zhang, Shoubo,Zhao, Yuanshu,Lei, Bin,Li, Chunjing,&Mao, Xiangming.(2015).PGAM1 is Involved in Spermatogenic Dysfunction and Affects Cell Proliferation, Apoptosis, and Migration.REPRODUCTIVE SCIENCES,22(10),1236-1242.
MLA Zhang, Shoubo,et al."PGAM1 is Involved in Spermatogenic Dysfunction and Affects Cell Proliferation, Apoptosis, and Migration".REPRODUCTIVE SCIENCES 22.10(2015):1236-1242.
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