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学科主题: 口腔医学
题名:
Using DGGE profiling to develop a novel culture medium suitable for oral microbial communities
作者: Tian, Y.4,5; He, X.1; Torralba, M.2; Yooseph, S.2; Nelson, K. E.2; Lux, R.1; McLean, J. S.3; Yu, G.4,5; Shi, W.1
关键词: growth medium ; oral microbial community ; 454 pyrosequencing
刊名: MOLECULAR ORAL MICROBIOLOGY
发表日期: 2010-10-01
卷: 25, 期:5, 页:357-367
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Dentistry, Oral Surgery & Medicine ; Microbiology
研究领域[WOS]: Dentistry, Oral Surgery & Medicine ; Microbiology
关键词[WOS]: GRADIENT GEL-ELECTROPHORESIS ; HUMAN DENTAL PLAQUE ; 16S RIBOSOMAL-RNA ; BACTERIAL COMMUNITY ; SUBGINGIVAL PLAQUE ; BIOFILMS ; DIVERSITY ; SALIVA ; GROWTH ; GENES
英文摘要:

P>More than 700 bacterial species have been detected in the human oral cavity. They form highly organized microbial communities and are responsible for many oral infectious diseases, such as dental caries and periodontal disease. The prevention and treatment of these diseases require a comprehensive knowledge of oral microbial communities, which largely relies on culture-dependent methods to provide detailed phenotypic and physiological analysis of these communities. However, most of the currently available laboratory media can only selectively support the growth of a limited number of bacterial species within these communities, and fail to sustain the original oral microbial diversity. In this study, using denaturing gradient gel electrophoresis (DGGE) as an index to systematically survey and analyse the selectivity of commonly used laboratory media, we developed a new medium (SHI medium) by combining the ingredients of several selected media that can support different subpopulations within the original oral microbial community derived from pooled saliva. DGGE and 454 pyrosequencing analysis showed that SHI medium was capable of supporting a more diversified community with a microbial profile closer to that of the original oral microbiota. Furthermore, 454 pyrosequencing revealed that SHI medium supported the growth of many oral species that have not before been cultured. Crystal violet assay and the confocal laser scanning microscope analysis indicated that, compared with other media, SHI medium is able to support a more complex saliva-derived biofilm with higher biomass yield and more diverse species. This DGGE-guided method could also be used to develop novel media for other complex microbial communities.

语种: 英语
所属项目编号: GM54666 ; DE020102 ; N01-AI-30071
项目资助者: National Institutes of Health ; National Institute of Allergy and Infectious Diseases, National Institutes of Health, Department of Health and Human Services
WOS记录号: WOS:000281551700005
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/61365
Appears in Collections:北京大学口腔医学院_期刊论文

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作者单位: 1.J Craig Venter Inst, Rockville, MD USA
2.J Craig Venter Inst, La Jolla, CA USA
3.Peking Univ, Sch Stomatol, Beijing 100871, Peoples R China
4.Peking Univ, Hosp Stomatol, Beijing 100871, Peoples R China
5.Univ Calif Los Angeles, Sch Dent, Los Angeles, CA 90095 USA

Recommended Citation:
Tian, Y.,He, X.,Torralba, M.,et al. Using DGGE profiling to develop a novel culture medium suitable for oral microbial communities[J]. MOLECULAR ORAL MICROBIOLOGY,2010,25(5):357-367.
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