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Up-regulation of cartilage oligomeric matrix protein gene expression by insulin-like growth factor-I revealed by real time reverse transcription-polymerase chain reaction
Tian, Hua; Stogiannidis, Ioannis
关键词cartilage oligomeric matrix protein (COMP) insulin-like growth factor-I (IGF-I) gene expression
刊名ACTA BIOCHIMICA ET BIOPHYSICA SINICA
2006-10-01
DOI10.1111/j.1745-7270.2006.00218.x
38期:10页:677-682
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]MULTIPLE EPIPHYSEAL DYSPLASIA ; ARTICULAR-CARTILAGE ; REPAIR ; PROLIFERATION ; COMP
英文摘要

Cartilage oligomeric matrix protein (COMP) strengthens cartilage by binding to type II and type IX collagen-forming bridges between collagen fibrils. It was hypothesized that perhaps one or more anabolic growth factors such as insulin-like growth factor-I (IGF-I), fibroblast growth factor-1 (FGF-1) or platelet derived growth factor-BB (PDGF-BB) increase COMP gene expression. Their effects on primary human chondrocytes and the chondrogenic cell line ATDC5 were studied using real time reverse transcript-polymerase chain reaction (RT-PCR) for quantification. IGF-I, but not the FGF-1 or PDGF-BB, up-regulated COMP gene expression by approximate 5-fold in human adult chondrocytes in a dose- and time-dependent manner. IGF-I exerted similar effects on ATDC5 cells. Results from these real time RT-PCR experiments were confirmed by transfecting into ATDC5 cells a full-length mouse COMP promoter cloned upstream of a luciferase reporter gene. On stimulation with IGF-I, the luciferase reporter activity increased by about eight times. In conclusion, IGF-I seems to be an important positive regulator of COMP, which may play an important role in an attempted repair of either traumatized or degenerated cartilage.

语种英语
WOS记录号WOS:000241620100002
引用统计
被引频次:6[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/61367
专题北京大学第三临床医学院_骨科
作者单位1.Coxa Hosp Joint Replacement, Tampere 33101, Finland
2.Peking Univ, Third Hosp, Dept Orthopaed, Beijing 100083, Peoples R China
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Tian, Hua,Stogiannidis, Ioannis. Up-regulation of cartilage oligomeric matrix protein gene expression by insulin-like growth factor-I revealed by real time reverse transcription-polymerase chain reaction[J]. ACTA BIOCHIMICA ET BIOPHYSICA SINICA,2006,38(10):677-682.
APA Tian, Hua,&Stogiannidis, Ioannis.(2006).Up-regulation of cartilage oligomeric matrix protein gene expression by insulin-like growth factor-I revealed by real time reverse transcription-polymerase chain reaction.ACTA BIOCHIMICA ET BIOPHYSICA SINICA,38(10),677-682.
MLA Tian, Hua,et al."Up-regulation of cartilage oligomeric matrix protein gene expression by insulin-like growth factor-I revealed by real time reverse transcription-polymerase chain reaction".ACTA BIOCHIMICA ET BIOPHYSICA SINICA 38.10(2006):677-682.
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