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学科主题: 基础医学
题名:
Compression and crevasses in vitreous sections under different cutting conditions
作者: Han, H. -M.1,2; Zuber, B.1; Dubochet, J.1
关键词: CEMOVIS ; compression ; crevasses ; cryo-ultramicrotomy
刊名: JOURNAL OF MICROSCOPY
发表日期: 2008-05-01
卷: 230, 期:2, 页:167-171
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Microscopy
研究领域[WOS]: Microscopy
关键词[WOS]: CRYOELECTRON MICROSCOPY ; SPECIMENS ; ARTIFACTS
英文摘要:

Compression and crevasses are common cutting artefacts in cryo-ultramicrotomy of vitreous sections. They can be reduced or suppressed under optimal cutting conditions. In the present study, compression and thickness were measured for different cutting speeds and knife angles. It was found that compression decreased with feed and that crevasses appeared only above a certain thickness. The optimal feed for vitreous sections was between 50 and 80 nm. The thickness, calculated by two independent methods, was quantitatively related to feed and compression.

语种: 英语
WOS记录号: WOS:000255286500001
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/61378
Appears in Collections:基础医学院_生物物理学系_期刊论文

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作者单位: 1.Univ Lausanne, Lab Anal Ultrastruct, CH-1015 Lausanne, Switzerland
2.Peking Univ, Hlth Sci Ctr, Dept Biophys, Beijing 100083, Peoples R China

Recommended Citation:
Han, H. -M.,Zuber, B.,Dubochet, J.. Compression and crevasses in vitreous sections under different cutting conditions[J]. JOURNAL OF MICROSCOPY,2008,230(2):167-171.
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