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学科主题基础医学
Compression and crevasses in vitreous sections under different cutting conditions
Han, H. -M.1,2; Zuber, B.1; Dubochet, J.1
关键词CEMOVIS compression crevasses cryo-ultramicrotomy
刊名JOURNAL OF MICROSCOPY
2008-05-01
230期:2页:167-171
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Microscopy
研究领域[WOS]Microscopy
关键词[WOS]CRYOELECTRON MICROSCOPY ; SPECIMENS ; ARTIFACTS
英文摘要

Compression and crevasses are common cutting artefacts in cryo-ultramicrotomy of vitreous sections. They can be reduced or suppressed under optimal cutting conditions. In the present study, compression and thickness were measured for different cutting speeds and knife angles. It was found that compression decreased with feed and that crevasses appeared only above a certain thickness. The optimal feed for vitreous sections was between 50 and 80 nm. The thickness, calculated by two independent methods, was quantitatively related to feed and compression.

语种英语
WOS记录号WOS:000255286500001
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被引频次:21[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/61378
专题北京大学基础医学院_生物物理学系
作者单位1.Univ Lausanne, Lab Anal Ultrastruct, CH-1015 Lausanne, Switzerland
2.Peking Univ, Hlth Sci Ctr, Dept Biophys, Beijing 100083, Peoples R China
推荐引用方式
GB/T 7714
Han, H. -M.,Zuber, B.,Dubochet, J.. Compression and crevasses in vitreous sections under different cutting conditions[J]. JOURNAL OF MICROSCOPY,2008,230(2):167-171.
APA Han, H. -M.,Zuber, B.,&Dubochet, J..(2008).Compression and crevasses in vitreous sections under different cutting conditions.JOURNAL OF MICROSCOPY,230(2),167-171.
MLA Han, H. -M.,et al."Compression and crevasses in vitreous sections under different cutting conditions".JOURNAL OF MICROSCOPY 230.2(2008):167-171.
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