学科主题基础医学
CKLFSF2 is highly expressed in testis and can be secreted into the seminiferous tubules
Shi, S; Rui, M; Han, WL; Wang, Y; Qiu, XY; Ding, PG; Zhang, P; Zhu, XH; Zhang, YM; Gan, QN; Ma, DL
关键词CKLFSF CKLFSF2 testis spermatogenesis
刊名INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
2005-08-01
DOI10.1016/j.biocel.2004.04.028
37期:8页:1633-1640
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Cell Biology
研究领域[WOS]Biochemistry & Molecular Biology ; Cell Biology
关键词[WOS]CHEMOKINE-LIKE FACTOR ; MOLECULAR-CLONING ; IN-VIVO ; RAT ; PROTEINS ; ISOFORMS ; FLUID ; CELLS
英文摘要

CKLFSF2 is a member of the chemokine-like factor superfamily (CKLFSF), a novel gene family containing CKLF and CKLFSF1-8. Using a combination of data mining and polymerase chain reactions, we determined the full cDNA sequence and genomic structure of human CKLFSF2, a 4-exon gene encoding 248 amino acids and spanning approximately 8.8 kb on chromosome 16q22.1 Expression profile analyses indicated that CKLFSF2 is expressed in a limited number of tissues. Specifically, immunohistochemistry indicated that CKLFSF2 is highly expressed in testis, mainly in spermatogonia and the seminiferous tubular fluid. Subcellular localization experiments suggested that CKLFSF2 is equally distributed in the cytoplasm, and Western blot analysis revealed that overexpressed CKLFSF2 is secreted into the supernatant of cultured cells. The data therefore strongly suggest that CKLFSF2 is a secreted protein that may be functionally relevant during spermatogenesis. (C) 2005 Elsevier Ltd. All rights reserved.

语种英语
WOS记录号WOS:000229709900011
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被引频次:23[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/61380
专题北京大学基础医学院_北京大学人类疾病基因研究中心
作者单位1.Peking Univ, Ctr Human Dis Genom, Beijing 100083, Peoples R China
2.Peking Univ, Sch Basic Med Sci, Lab Med Immunol, Beijing 100083, Peoples R China
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GB/T 7714
Shi, S,Rui, M,Han, WL,et al. CKLFSF2 is highly expressed in testis and can be secreted into the seminiferous tubules[J]. INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY,2005,37(8):1633-1640.
APA Shi, S.,Rui, M.,Han, WL.,Wang, Y.,Qiu, XY.,...&Ma, DL.(2005).CKLFSF2 is highly expressed in testis and can be secreted into the seminiferous tubules.INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY,37(8),1633-1640.
MLA Shi, S,et al."CKLFSF2 is highly expressed in testis and can be secreted into the seminiferous tubules".INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY 37.8(2005):1633-1640.
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