北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 基础医学院  > 北京大学人类疾病基因研究中心  > 期刊论文
学科主题: 基础医学
题名:
CKLFSF2 is highly expressed in testis and can be secreted into the seminiferous tubules
作者: Shi, S; Rui, M; Han, WL; Wang, Y; Qiu, XY; Ding, PG; Zhang, P; Zhu, XH; Zhang, YM; Gan, QN; Ma, DL
关键词: CKLFSF ; CKLFSF2 ; testis ; spermatogenesis
刊名: INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
发表日期: 2005-08-01
DOI: 10.1016/j.biocel.2004.04.028
卷: 37, 期:8, 页:1633-1640
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Cell Biology
研究领域[WOS]: Biochemistry & Molecular Biology ; Cell Biology
关键词[WOS]: CHEMOKINE-LIKE FACTOR ; MOLECULAR-CLONING ; IN-VIVO ; RAT ; PROTEINS ; ISOFORMS ; FLUID ; CELLS
英文摘要:

CKLFSF2 is a member of the chemokine-like factor superfamily (CKLFSF), a novel gene family containing CKLF and CKLFSF1-8. Using a combination of data mining and polymerase chain reactions, we determined the full cDNA sequence and genomic structure of human CKLFSF2, a 4-exon gene encoding 248 amino acids and spanning approximately 8.8 kb on chromosome 16q22.1 Expression profile analyses indicated that CKLFSF2 is expressed in a limited number of tissues. Specifically, immunohistochemistry indicated that CKLFSF2 is highly expressed in testis, mainly in spermatogonia and the seminiferous tubular fluid. Subcellular localization experiments suggested that CKLFSF2 is equally distributed in the cytoplasm, and Western blot analysis revealed that overexpressed CKLFSF2 is secreted into the supernatant of cultured cells. The data therefore strongly suggest that CKLFSF2 is a secreted protein that may be functionally relevant during spermatogenesis. (C) 2005 Elsevier Ltd. All rights reserved.

语种: 英语
WOS记录号: WOS:000229709900011
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/61380
Appears in Collections:基础医学院_北京大学人类疾病基因研究中心_期刊论文

Files in This Item:
File Name/ File Size Content Type Version Access License
CKLFSF2 is highly expressed in testis and can be secreted into the seminiferous tubules.pdf(372KB)期刊论文出版稿限制开放 联系获取全文

作者单位: 1.Peking Univ, Ctr Human Dis Genom, Beijing 100083, Peoples R China
2.Peking Univ, Sch Basic Med Sci, Lab Med Immunol, Beijing 100083, Peoples R China

Recommended Citation:
Shi, S,Rui, M,Han, WL,et al. CKLFSF2 is highly expressed in testis and can be secreted into the seminiferous tubules[J]. INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY,2005,37(8):1633-1640.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Shi, S]'s Articles
[Rui, M]'s Articles
[Han, WL]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Shi, S]‘s Articles
[Rui, M]‘s Articles
[Han, WL]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace