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学科主题基础医学
Last intron of the chemokine-like factor gene contains a putative promoter for the downstream CKLF super family member 1 gene
Xu, MX; Han, WL; Qian, MP; Ma, XT; Ding, PG; Wang, Y; Xia, DL; Rui, M; Wang, L; Zhang, YM; Ma, DL
关键词chemokine-like factor chemokine-like factor super family luciferase assay intron
刊名BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
2004-01-02
DOI10.1016/j.bbrc.2003.11.100
313期:1页:135-141
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]GROWTH-HORMONE GENE ; 1ST INTRON ; TRANSCRIPTION FACTORS ; EUKARYOTIC PROMOTER ; RESPONSE ELEMENT ; MESSENGER-RNA ; SEQUENCES ; IDENTIFICATION ; EXPRESSION ; ENHANCER
英文摘要

The genes for chemokine-like factor (CKLF) and four chemokine-like factor super family members (CKLFSF1-4) are tightly linked on chromosome 16, with only 325 bp separating CKLF and CKLFSF1. We used Northern blotting and RT-PCR to show that these two genes are expressed independently of one another. We then used a novel computational promoter prediction method based on the interaction among transcription factor binding sites (TFBSs) to identify a putative promoter region for the CKLFSF1 gene. Our method predicted a promoter region in the last intron of the upstream gene, CKLF. We PCR amplified the predicted promoter region and used a luciferase assay to show that the region was able to drive the luciferase gene. DNA decoy experiments indicated that 214 bp fragment neighboring the TATA box markedly inhibited CKLFSF1 gene expression. Sequence analysis of the region revealed a typical TATA box (TATATAA) and multiple potential transcription factor binding sites, providing further evidence for this being a functional promoter for, CKLFSF1. This work provides the first evidence of a promoter from one gene located in an intron of another. (C) 2003 Elsevier Inc. All rights reserved.

语种英语
WOS记录号WOS:000187745900022
Citation statistics
Cited Times:7[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/61387
Collection北京大学基础医学院_免疫学系
作者单位1.Peking Univ, Sch Basic Med Sci, Lab Med Immunol, Beijing 100083, Peoples R China
2.Peking Univ, Sch Math, Beijing 100871, Peoples R China
Recommended Citation
GB/T 7714
Xu, MX,Han, WL,Qian, MP,et al. Last intron of the chemokine-like factor gene contains a putative promoter for the downstream CKLF super family member 1 gene[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2004,313(1):135-141.
APA Xu, MX.,Han, WL.,Qian, MP.,Ma, XT.,Ding, PG.,...&Ma, DL.(2004).Last intron of the chemokine-like factor gene contains a putative promoter for the downstream CKLF super family member 1 gene.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,313(1),135-141.
MLA Xu, MX,et al."Last intron of the chemokine-like factor gene contains a putative promoter for the downstream CKLF super family member 1 gene".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 313.1(2004):135-141.
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