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学科主题: 基础医学
题名:
Last intron of the chemokine-like factor gene contains a putative promoter for the downstream CKLF super family member 1 gene
作者: Xu, MX; Han, WL; Qian, MP; Ma, XT; Ding, PG; Wang, Y; Xia, DL; Rui, M; Wang, L; Zhang, YM; Ma, DL
关键词: chemokine-like factor ; chemokine-like factor super family ; luciferase assay ; intron
刊名: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
发表日期: 2004-01-02
DOI: 10.1016/j.bbrc.2003.11.100
卷: 313, 期:1, 页:135-141
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]: Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]: GROWTH-HORMONE GENE ; 1ST INTRON ; TRANSCRIPTION FACTORS ; EUKARYOTIC PROMOTER ; RESPONSE ELEMENT ; MESSENGER-RNA ; SEQUENCES ; IDENTIFICATION ; EXPRESSION ; ENHANCER
英文摘要:

The genes for chemokine-like factor (CKLF) and four chemokine-like factor super family members (CKLFSF1-4) are tightly linked on chromosome 16, with only 325 bp separating CKLF and CKLFSF1. We used Northern blotting and RT-PCR to show that these two genes are expressed independently of one another. We then used a novel computational promoter prediction method based on the interaction among transcription factor binding sites (TFBSs) to identify a putative promoter region for the CKLFSF1 gene. Our method predicted a promoter region in the last intron of the upstream gene, CKLF. We PCR amplified the predicted promoter region and used a luciferase assay to show that the region was able to drive the luciferase gene. DNA decoy experiments indicated that 214 bp fragment neighboring the TATA box markedly inhibited CKLFSF1 gene expression. Sequence analysis of the region revealed a typical TATA box (TATATAA) and multiple potential transcription factor binding sites, providing further evidence for this being a functional promoter for, CKLFSF1. This work provides the first evidence of a promoter from one gene located in an intron of another. (C) 2003 Elsevier Inc. All rights reserved.

语种: 英语
WOS记录号: WOS:000187745900022
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/61387
Appears in Collections:基础医学院_免疫学系_期刊论文

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作者单位: 1.Peking Univ, Sch Basic Med Sci, Lab Med Immunol, Beijing 100083, Peoples R China
2.Peking Univ, Sch Math, Beijing 100871, Peoples R China

Recommended Citation:
Xu, MX,Han, WL,Qian, MP,et al. Last intron of the chemokine-like factor gene contains a putative promoter for the downstream CKLF super family member 1 gene[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2004,313(1):135-141.
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