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学科主题口腔医学
Force-induced Adrb2 in Periodontal Ligament Cells Promotes Tooth Movement
Cao, H.1,2; Kou, X.1,2; Yang, R.1,2; Liu, D.1,2; Wang, X.1,2; Song, Y.1,2; Feng, L.1,2; He, D.1,2; Gan, Y.3; Zhou, Y.1,2
关键词orthodontics biomechanical phenomena beta-2 adrenergic receptors sympathetic nervous system bone remodeling osteoclast
刊名JOURNAL OF DENTAL RESEARCH
2014-11-01
DOI10.1177/0022034514551769
93期:11页:1163-1169
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Dentistry, Oral Surgery & Medicine
研究领域[WOS]Dentistry, Oral Surgery & Medicine
关键词[WOS]SYMPATHETIC-NERVOUS-SYSTEM ; CREST STEM-CELLS ; BONE-FORMATION ; ADAPTIVE RESPONSE ; STIMULATION ; EXPRESSION ; DELETION ; CYCLASE ; MICE ; CAMP
英文摘要

The sympathetic nervous system (SNS) regulates bone resorption through -2 adrenergic receptor (Adrb2). In orthodontic tooth movement (OTM), mechanical force induces and regulates alveolar bone remodeling. Compressive force-associated osteoclast differentiation and alveolar bone resorption are the rate-limiting steps of tooth movement. However, whether mechanical force can activate Adrb2 and thus contribute to OTM remains unknown. In this study, orthodontic nickel-titanium springs were applied to the upper first molars of rats and Adrb1/2(-/-) mice to confirm the role of SNS and Adrb2 in OTM. The results showed that blockage of SNS activity in the jawbones of rats by means of superior cervical ganglion ectomy reduced OTM distance from 860 to 540 m after 14 d of force application. In addition, the injection of nonselective Adrb2 agonist isoproterenol activated the downstream signaling of SNS to accelerate OTM from 300 to 540 m after 7 d of force application. Adrb1/2(-/-) mice showed significantly reduced OTM distance (19.5 m) compared with the wild-type mice (107.6 m) after 7 d of force application. Histopathologic analysis showed that the number of Adrb2-positive cells increased in the compressive region of periodontal ligament after orthodontic force was applied on rats. Mechanistically, mechanical compressive force upregulated Adrb2 expression in primary-cultured human periodontal ligament cells (PDLCs) through the elevation of intracellular Ca2+ concentration. Activation of Adrb2 in PDLCs increased the RANKL/OPG ratio and promoted the peripheral blood mononuclear cell differentiation to osteoclasts in the cocultured system. Upregulation of Adrb2 in PDLCs promoted osteoclastogenesis, which accelerated OTM through Adrb2-enhanced bone resorption. In summary, this study suggests that mechanical force-induced Adrb2 activation in PDLCs contributes to SNS-regulated OTM.

语种英语
WOS记录号WOS:000343975100017
引用统计
被引频次:18[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/61399
专题北京大学口腔医学院_牙周科
北京大学基础医学院
作者单位1.Peking Univ, Sch & Hosp Stomatol, Dept Orthodont, Beijing 100871, Peoples R China
2.Peking Univ, Sch & Hosp Stomatol, Ctr Craniofacial Stem Cell Res & Regenerat, Beijing 100871, Peoples R China
3.Peking Univ, Sch & Hosp Stomatol, Ctr Temporomandibular Disorders & Orofacial Pain, Beijing 100871, Peoples R China
推荐引用方式
GB/T 7714
Cao, H.,Kou, X.,Yang, R.,et al. Force-induced Adrb2 in Periodontal Ligament Cells Promotes Tooth Movement[J]. JOURNAL OF DENTAL RESEARCH,2014,93(11):1163-1169.
APA Cao, H..,Kou, X..,Yang, R..,Liu, D..,Wang, X..,...&Zhou, Y..(2014).Force-induced Adrb2 in Periodontal Ligament Cells Promotes Tooth Movement.JOURNAL OF DENTAL RESEARCH,93(11),1163-1169.
MLA Cao, H.,et al."Force-induced Adrb2 in Periodontal Ligament Cells Promotes Tooth Movement".JOURNAL OF DENTAL RESEARCH 93.11(2014):1163-1169.
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