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Maintaining hyporesponsiveness and polarization potential of T cells after in vitro mixture of G-CSF mobilized peripheral blood grafts and G-CSF primed bone marrow grafts in different proportions
Huang, Xiao-Jun; Chang, Ying Jun; Zhao, Xiang Yu
关键词bone marrow grafts peripheral blood stem cells grafts granulocyte colony-stimulating factor dendritic cells T cell
刊名TRANSPLANT IMMUNOLOGY
2007-04-01
DOI10.1016/j.trim.2006.10.002
17期:3页:193-197
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Immunology ; Transplantation
研究领域[WOS]Immunology ; Transplantation
关键词[WOS]COLONY-STIMULATING FACTOR ; VERSUS-HOST-DISEASE ; TRANSPLANTATION
英文摘要

Background and objectives: Granulocyte colony-stimulating factor (G-CSF) primed bone marrow grafts (G-BM) plus G-CSF mobilized peripheral blood grafts (G-PB) were used successfully in haploidentical transplantation and the incidence of graft-versus-host disease (GVHD) was not higher compared with that in patients with HLA-matched donors. The immunological characteristics of T cells in G-BM and G-PB mixed in vitro in different proportions were investigated.

Design and methods: Lymphocyte proliferation ability, interferon-gamma (IFN-gamma) and interleukin-4 (IL-4) secreted by T cells were determined using a monotetrazolium (MTT) assay and sandwich enzyme-linked immunosorbent assay (ELISA), respectively. T cell subgroups, dendritic cells (DC) subsets, and monocytes were analyzed using flow cytometry in steady-state bone marrow (SS-BM), G-PB, and G-BM and calculated in mixture grafts.

Results: The quantities of IFN-gamma and IL-4 secreted by lymphocytes per microliter in the three mixture grafts were 2- to 4-fold lower than in G-PB and 1- to 3-fold higher than in SS-BM and G-BM, while the IL-4/IFN-gamma ratio was higher than SS-BM and G-PB and lower than GBM, although no significant difference was confirmed. Lymphocyte proliferation ability in the three mixture grafts was comparable to G-BM and significantly lower than SS-BM and G-PB. Lymphocytes, monocytes, T cell subsets, and DC subsets were 2- to 8-fold lower than in G-PB and 2- to 22-fold higher than in SS-BM and G-BM. The DC1/DC2 ratio was significantly higher in SS-BM than G-PB, G-BM, and the three mixture grafts (P<0.05).

Interpretation and conclusions: Our results suggest that T cell hyporesponsiveness and polarization of T cell from Th1 to Th2 could be maintained after in vitro mixture of G-PB and G-BM in different proportions. (C) 2006 Elsevier B.V All rights reserved.

语种英语
WOS记录号WOS:000245370900007
引用统计
被引频次:47[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/61414
专题北京大学第二临床医学院_血液科
作者单位Peking Univ, Inst Hematol, Peoples Hosp, Beijing 100044, Peoples R China
推荐引用方式
GB/T 7714
Huang, Xiao-Jun,Chang, Ying Jun,Zhao, Xiang Yu. Maintaining hyporesponsiveness and polarization potential of T cells after in vitro mixture of G-CSF mobilized peripheral blood grafts and G-CSF primed bone marrow grafts in different proportions[J]. TRANSPLANT IMMUNOLOGY,2007,17(3):193-197.
APA Huang, Xiao-Jun,Chang, Ying Jun,&Zhao, Xiang Yu.(2007).Maintaining hyporesponsiveness and polarization potential of T cells after in vitro mixture of G-CSF mobilized peripheral blood grafts and G-CSF primed bone marrow grafts in different proportions.TRANSPLANT IMMUNOLOGY,17(3),193-197.
MLA Huang, Xiao-Jun,et al."Maintaining hyporesponsiveness and polarization potential of T cells after in vitro mixture of G-CSF mobilized peripheral blood grafts and G-CSF primed bone marrow grafts in different proportions".TRANSPLANT IMMUNOLOGY 17.3(2007):193-197.
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