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Effect of beta-escin sodium on endothelial cells proliferation, migration and apoptosis
Wang, Xu-Hua; Xu, Bo; Liu, Jing-Tao; Cui, Jing-Rong
关键词Angiogenesis beta-Escin Proliferation Migration Apoptosis
刊名VASCULAR PHARMACOLOGY
2008-10-01
DOI10.1016/j.vph.2008.07.005
49期:4-6页:158-165
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Pharmacology & Pharmacy
研究领域[WOS]Pharmacology & Pharmacy
关键词[WOS]FIBROBLAST-GROWTH-FACTOR ; PROTEIN-KINASE-C ; TUMOR ANGIOGENESIS ; INHIBITS ANGIOGENESIS ; THROMBOSPONDIN-1 ; CANCER ; MECHANISMS ; ECV304 ; ACTIVATION ; KDR/FLK-1
英文摘要

beta-Escin, the major active compound in extracts of the horse chestnut Aesculus hippocastanum seed, has shown clinically significant activity in chronic venous insufficiency (CVI). Our previous studies had shown that beta-escin sodium inhibited angiogenesis in chick chorioallantoic membrane (CAM) and in aortic disk assay. In this study, we explored the direct effect of beta-escin sodium on proliferation, migration and apoptosis in human umbilical vein endothelial cells (HUVECs) and ECV304 cells. Sulforhodamine B (SRB) assay showed that beta-escin sodium (10, 20, 40 mu g/ml) inhibited endothelial cells (ECs) proliferation dose-dependently. beta-escin sodium also induced ECs apoptosis at 40 mu g/ml. Cell migration was evaluated by an improved wound assay: barren spot assay. And the direct effect on cell motility excluding influence of cell proliferation was examined by High Content Screening (HCS, Cellomics) assay. The data indicated that beta-escin sodium suppressed ECs migration and cell motility. Western blot results suggested that beta-escin sodium acts on ECs possibly by increasing expression of thrombospondin-1 (TSP-1), and decreasing expression of PKC-alpha and activation of p44/42 mitogen-activated protein kinase (ERK) and p38 mitogen-activated protein kinase (p38 MAPK). Our findings give the evidence that beta-escin sodium might have potential anti-angiogenic activity via its direct effects on ECs. (C) 2008 Published by Elsevier Inc.

语种英语
WOS记录号WOS:000261126300007
引用统计
被引频次:29[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/61417
专题北京大学药学院
北京大学药学院_分子与细胞药理学系
北京大学临床肿瘤学院_肿瘤放疗科
作者单位Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet, Beijing 100083, Peoples R China
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GB/T 7714
Wang, Xu-Hua,Xu, Bo,Liu, Jing-Tao,et al. Effect of beta-escin sodium on endothelial cells proliferation, migration and apoptosis[J]. VASCULAR PHARMACOLOGY,2008,49(4-6):158-165.
APA Wang, Xu-Hua,Xu, Bo,Liu, Jing-Tao,&Cui, Jing-Rong.(2008).Effect of beta-escin sodium on endothelial cells proliferation, migration and apoptosis.VASCULAR PHARMACOLOGY,49(4-6),158-165.
MLA Wang, Xu-Hua,et al."Effect of beta-escin sodium on endothelial cells proliferation, migration and apoptosis".VASCULAR PHARMACOLOGY 49.4-6(2008):158-165.
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