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学科主题: 药学
题名:
Effect of beta-escin sodium on endothelial cells proliferation, migration and apoptosis
作者: Wang, Xu-Hua; Xu, Bo; Liu, Jing-Tao; Cui, Jing-Rong
关键词: Angiogenesis ; beta-Escin ; Proliferation ; Migration ; Apoptosis
刊名: VASCULAR PHARMACOLOGY
发表日期: 2008-10-01
DOI: 10.1016/j.vph.2008.07.005
卷: 49, 期:4-6, 页:158-165
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Pharmacology & Pharmacy
研究领域[WOS]: Pharmacology & Pharmacy
关键词[WOS]: FIBROBLAST-GROWTH-FACTOR ; PROTEIN-KINASE-C ; TUMOR ANGIOGENESIS ; INHIBITS ANGIOGENESIS ; THROMBOSPONDIN-1 ; CANCER ; MECHANISMS ; ECV304 ; ACTIVATION ; KDR/FLK-1
英文摘要:

beta-Escin, the major active compound in extracts of the horse chestnut Aesculus hippocastanum seed, has shown clinically significant activity in chronic venous insufficiency (CVI). Our previous studies had shown that beta-escin sodium inhibited angiogenesis in chick chorioallantoic membrane (CAM) and in aortic disk assay. In this study, we explored the direct effect of beta-escin sodium on proliferation, migration and apoptosis in human umbilical vein endothelial cells (HUVECs) and ECV304 cells. Sulforhodamine B (SRB) assay showed that beta-escin sodium (10, 20, 40 mu g/ml) inhibited endothelial cells (ECs) proliferation dose-dependently. beta-escin sodium also induced ECs apoptosis at 40 mu g/ml. Cell migration was evaluated by an improved wound assay: barren spot assay. And the direct effect on cell motility excluding influence of cell proliferation was examined by High Content Screening (HCS, Cellomics) assay. The data indicated that beta-escin sodium suppressed ECs migration and cell motility. Western blot results suggested that beta-escin sodium acts on ECs possibly by increasing expression of thrombospondin-1 (TSP-1), and decreasing expression of PKC-alpha and activation of p44/42 mitogen-activated protein kinase (ERK) and p38 mitogen-activated protein kinase (p38 MAPK). Our findings give the evidence that beta-escin sodium might have potential anti-angiogenic activity via its direct effects on ECs. (C) 2008 Published by Elsevier Inc.

语种: 英语
WOS记录号: WOS:000261126300007
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/61417
Appears in Collections:北京大学药学院_期刊论文

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作者单位: Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet, Beijing 100083, Peoples R China

Recommended Citation:
Wang, Xu-Hua,Xu, Bo,Liu, Jing-Tao,et al. Effect of beta-escin sodium on endothelial cells proliferation, migration and apoptosis[J]. VASCULAR PHARMACOLOGY,2008,49(4-6):158-165.
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