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学科主题口腔医学
COX2 is involved in hypoxia-induced TNF-alpha expression in osteoblast
Xing, Yonggang1; Wang, Renxian2; Chen, Dafu2; Mao, Jianping1; Shi, Rui2; Wu, Zhihong3,4; Kang, Jun5; Tian, Wei1; Zhang, Chi6
刊名SCIENTIFIC REPORTS
2015-06-12
DOI10.1038/srep10020
5
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Multidisciplinary Sciences
资助者National Natural Science Foundation of China ; National Natural Science Foundation of China
研究领域[WOS]Science & Technology - Other Topics
关键词[WOS]FACTOR OSTERIX OSX ; TRANSCRIPTIONAL REGULATION ; SKELETAL DEVELOPMENT ; BONE-FORMATION ; VEGF ; ANGIOGENESIS ; OSTEOGENESIS ; DISEASE
英文摘要

Bone regeneration involves a series of events in a coordinated manner, including recruitment of mesenchymal stem cells, induction of immune response, inflammatory activity and vascular ingrowth. The microenvironment of bone regeneration is hypoxic. Low oxygen tension (hypoxia) promotes the upregulation of several signaling molecules. The primary mediating factor is the hypoxia-inducible factor-1 (HIF-1). Hypoxia stimulates the expression of a variety of cytokines from inflammatory cells, fibroblasts, endothelial cells, and osteoblasts. TNF-alpha is a key proinflammatory cytokine. The molecular events involved in osteoblast dysfunction under hypoxia are not fully understood. This study determined the effects of hypoxia on TNF-alpha in osteoblasts, and molecular mechanisms were explored. We observed that hypoxia induced TNF-alpha expression in a time-dependent manner in osteoblasts. Experiments using a potent HIF-1 alpha activator DFO demonstrated that hypoxia-induced TNF-alpha was mediated by HIF-1-alpha. In addition, this study showed that hypoxia activated cyclooxygenase-2 (COX2) expression along with TNF-alpha. Inhibition experiments using COX2 inhibitor N398 indicated that COX2 was involved in hypoxia-mediated TNF-alpha expression, and this observation was further confirmed by Small interfering RNA against COX2. On the other hand, TNF-alpha didn′t lead to the activation of COX2 expression. We conclude that COX2 is involved in hypoxia-induced TNF-alpha expression in osteoblast.

语种英语
所属项目编号81171682 ; 31430030 ; 81271942
资助者National Natural Science Foundation of China ; National Natural Science Foundation of China
WOS记录号WOS:000356136300001
引用统计
被引频次:2[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/61423
专题北京大学口腔医学院_牙周科
作者单位1.Beijing JiShuiTan Hosp, Dept Spine, Beijing 100035, Peoples R China
2.Beijing JiShuiTan Hosp, Beijing Res Inst Traumatol & Orthopaed, Lab Bone Tissue Engn, Beijing Lab Biomed Mat, Beijing 100035, Peoples R China
3.Beijing Union Med Coll Hosp, Peking Union Med Coll, Dept Orthoped Surg, Beijing 100730, Peoples R China
4.Chinese Acad Med Sci, Beijing 100730, Peoples R China
5.Peking Univ, Sch & Hosp Stomatol, Dept Periodontol, Beijing 100081, Peoples R China
6.Univ Texas SW Med Ctr Dallas, Bone Res Lab, Dallas, TX 75390 USA
推荐引用方式
GB/T 7714
Xing, Yonggang,Wang, Renxian,Chen, Dafu,et al. COX2 is involved in hypoxia-induced TNF-alpha expression in osteoblast[J]. SCIENTIFIC REPORTS,2015,5.
APA Xing, Yonggang.,Wang, Renxian.,Chen, Dafu.,Mao, Jianping.,Shi, Rui.,...&Zhang, Chi.(2015).COX2 is involved in hypoxia-induced TNF-alpha expression in osteoblast.SCIENTIFIC REPORTS,5.
MLA Xing, Yonggang,et al."COX2 is involved in hypoxia-induced TNF-alpha expression in osteoblast".SCIENTIFIC REPORTS 5(2015).
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