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学科主题: 基础医学
题名:
Salmonella expressing a T-cell epitope from Sendai virus are able to induce anti-infection immunity
作者: Huang, He1,2; Wang, Ye-Jun1; White, Aaron P.3; Meng, Jia-Zi2,4; Liu, Gui-Rong1,3; Liu, Shu-Lin1,3,5; Wang, Yue-Dan2,4
刊名: JOURNAL OF MEDICAL MICROBIOLOGY
发表日期: 2009-09-01
DOI: 10.1099/jmm.0.011767-0
卷: 58, 期:9, 页:1236-1242
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Microbiology
研究领域[WOS]: Microbiology
关键词[WOS]: ATTENUATED SALMONELLA ; DNA VACCINE ; AGGREGATIVE FIMBRIAE ; ORAL IMMUNIZATION ; GENE REPLACEMENT ; TYPHIMURIUM ; MICE ; ENTERITIDIS ; INFECTION ; ANTIGENS
英文摘要:

Bacterial fimbriae can accept foreign peptides, and display them on the cell surface. A highly efficient gene replacement method was used to generate peptide vaccines based on Salmonella enterica subsp. enterica serovar Typhimurium LT2. DNA encoding an epitope from Sendai virus, SV9 (Sendai virus nucleoprotein peptide 324-332, FAPGNYPAL), which is known to induce cytotoxic T lymphocytes, was incorporated into the gene encoding AgfA (the major subunit protein of thin aggregative fimbriae of Salmonella) by replacing an equal length DNA segment. To improve cytotoxic T lymphocyte recognition, both termini of the peptide were flanked by double alanine (AA) or arginine (RR) residues. Western blotting and immunofluorescence microscopy using AgfA-specific antiserum verified the expression of chimeric AgfA; expression was also proved by a Congo red binding assay. Oral immunizations of C57BL/6 mice with the four strains induced an epitope-specific T-cell response (detected by enzyme-linked immunosorbent spot assay). When the mice were challenged with the Sendai virus, the magnitude of the infection was significantly reduced in the immunized groups compared with the controls. The Salmonella fimbrial display system efficiently induces a cellular immune response and anti-infection immunity in vivo, providing a new strategy for the development of efficient peptide vaccination.

语种: 英语
所属项目编号: 30771995 ; 30671962 ; J0630853 ; J0108
项目资助者: NSFC (Natural Science Foundation of China) ; NFFTBS (National Science Fund for Fostering Talent in Basic Science)
WOS记录号: WOS:000269676200018
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/61427
Appears in Collections:基础医学院_病原生物学系_期刊论文

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作者单位: 1.Univ Calgary, Dept Microbiol & Infect Dis, Calgary, AB, Canada
2.Peking Univ, Dept Microbiol, Hlth Sci Ctr, Beijing 100871, Peoples R China
3.Peking Univ, Hlth Sci Ctr, Lab Immunol & Microbiol, BioMed Educ Ctr, Beijing 100871, Peoples R China
4.Peking Univ, Dept Immunol, Hlth Sci Ctr, Beijing 100871, Peoples R China
5.Harbin Med Coll, Genom Res Ctr, Harbin 150081, Peoples R China

Recommended Citation:
Huang, He,Wang, Ye-Jun,White, Aaron P.,et al. Salmonella expressing a T-cell epitope from Sendai virus are able to induce anti-infection immunity[J]. JOURNAL OF MEDICAL MICROBIOLOGY,2009,58(9):1236-1242.
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