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Salmonella expressing a T-cell epitope from Sendai virus are able to induce anti-infection immunity
Huang, He1,2; Wang, Ye-Jun1; White, Aaron P.3; Meng, Jia-Zi2,4; Liu, Gui-Rong1,3; Liu, Shu-Lin1,3,5; Wang, Yue-Dan2,4
刊名JOURNAL OF MEDICAL MICROBIOLOGY
2009-09-01
DOI10.1099/jmm.0.011767-0
58期:9页:1236-1242
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Microbiology
资助者NSFC (Natural Science Foundation of China) ; NFFTBS (National Science Fund for Fostering Talent in Basic Science) ; NSFC (Natural Science Foundation of China) ; NFFTBS (National Science Fund for Fostering Talent in Basic Science)
研究领域[WOS]Microbiology
关键词[WOS]ATTENUATED SALMONELLA ; DNA VACCINE ; AGGREGATIVE FIMBRIAE ; ORAL IMMUNIZATION ; GENE REPLACEMENT ; TYPHIMURIUM ; MICE ; ENTERITIDIS ; INFECTION ; ANTIGENS
英文摘要

Bacterial fimbriae can accept foreign peptides, and display them on the cell surface. A highly efficient gene replacement method was used to generate peptide vaccines based on Salmonella enterica subsp. enterica serovar Typhimurium LT2. DNA encoding an epitope from Sendai virus, SV9 (Sendai virus nucleoprotein peptide 324-332, FAPGNYPAL), which is known to induce cytotoxic T lymphocytes, was incorporated into the gene encoding AgfA (the major subunit protein of thin aggregative fimbriae of Salmonella) by replacing an equal length DNA segment. To improve cytotoxic T lymphocyte recognition, both termini of the peptide were flanked by double alanine (AA) or arginine (RR) residues. Western blotting and immunofluorescence microscopy using AgfA-specific antiserum verified the expression of chimeric AgfA; expression was also proved by a Congo red binding assay. Oral immunizations of C57BL/6 mice with the four strains induced an epitope-specific T-cell response (detected by enzyme-linked immunosorbent spot assay). When the mice were challenged with the Sendai virus, the magnitude of the infection was significantly reduced in the immunized groups compared with the controls. The Salmonella fimbrial display system efficiently induces a cellular immune response and anti-infection immunity in vivo, providing a new strategy for the development of efficient peptide vaccination.

语种英语
所属项目编号30771995 ; 30671962 ; J0630853 ; J0108
资助者NSFC (Natural Science Foundation of China) ; NFFTBS (National Science Fund for Fostering Talent in Basic Science) ; NSFC (Natural Science Foundation of China) ; NFFTBS (National Science Fund for Fostering Talent in Basic Science)
WOS记录号WOS:000269676200018
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被引频次:6[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/61427
专题基础医学院_病原生物学系
作者单位1.Univ Calgary, Dept Microbiol & Infect Dis, Calgary, AB, Canada
2.Peking Univ, Dept Microbiol, Hlth Sci Ctr, Beijing 100871, Peoples R China
3.Peking Univ, Hlth Sci Ctr, Lab Immunol & Microbiol, BioMed Educ Ctr, Beijing 100871, Peoples R China
4.Peking Univ, Dept Immunol, Hlth Sci Ctr, Beijing 100871, Peoples R China
5.Harbin Med Coll, Genom Res Ctr, Harbin 150081, Peoples R China
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GB/T 7714
Huang, He,Wang, Ye-Jun,White, Aaron P.,et al. Salmonella expressing a T-cell epitope from Sendai virus are able to induce anti-infection immunity[J]. JOURNAL OF MEDICAL MICROBIOLOGY,2009,58(9):1236-1242.
APA Huang, He.,Wang, Ye-Jun.,White, Aaron P..,Meng, Jia-Zi.,Liu, Gui-Rong.,...&Wang, Yue-Dan.(2009).Salmonella expressing a T-cell epitope from Sendai virus are able to induce anti-infection immunity.JOURNAL OF MEDICAL MICROBIOLOGY,58(9),1236-1242.
MLA Huang, He,et al."Salmonella expressing a T-cell epitope from Sendai virus are able to induce anti-infection immunity".JOURNAL OF MEDICAL MICROBIOLOGY 58.9(2009):1236-1242.
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