北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学第一临床医学院  > 儿科  > 期刊论文
学科主题: 临床医学
题名:
Long-term Effects of Recurrent Neonatal Seizures on Neurobehavioral Function and Related Gene Expression and Its Intervention by Inhibitor of Cathepsin B
作者: Ni, Hong1,2; Yan, Jian-zhen1,2; Zhang, Le-ling1,2; Feng, Xing1,2; Wu, Xi-ru3
关键词: Cathepsin B ; Autophagy ; bcl-2 ; PRG-1 ; Seizure
刊名: NEUROCHEMICAL RESEARCH
发表日期: 2012
DOI: 10.1007/s11064-011-0578-z
卷: 37, 期:1, 页:31-39
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Neurosciences
研究领域[WOS]: Biochemistry & Molecular Biology ; Neurosciences & Neurology
关键词[WOS]: BRAIN-INJURY ; CELL-DEATH ; UP-REGULATION ; WISTAR RAT ; AUTOPHAGY ; HIPPOCAMPUS ; APOPTOSIS ; ISCHEMIA ; PRG-1 ; MITOCHONDRIA
英文摘要:

Cathepsins are families of proteases that have been reported to play the key roles in neuroexcitotoxicity. The present study was sought to determine the effect of CBI, a cathepsin B inhibitor, in the prevention of neurobehavioral deficits after inhalant flurothyl-induced recurrent neonatal seizures in rats. We examined the expression pattern of autophagy-related genes at acute phase after the last seizures using western blot method, and evaluated behavioral deficits during postnatal day 28 (P28) to P35. The results showed improved neurological scores and learning ability in CBI-treated rats compared with the nontreated control. Flurothyl-induced increases in the ratio of LC3-II/LC3-I, Beclin-1 and Cathepsin-B were blocked by pre-treatment with CBI at 1.5, 3, 6 and 24 h after the last seizures in hippocampus and cerebral cortex by western blot analysis. Meanwhile, CBI also reversed flurothyl-induced down-regulation of Bcl-2 protein levels. Furthermore, in the long-term time point of 35 days (P35), PRG-1 mRNA and protein level in hippocampus and cerebral cortex of recurrent seizure group were up-regulated when compared to the control rats; meanwhile, the up-regulated expression of PRG-1 were robustly inhibited by CBI. These date demonstrated, for the first time, that lysosomal enzymes participate in neonatal seizure-induced brain damage and that modulation of cathepsin B may offer a new strategy for the development of therapeutic interventions for treatment of developmental seizure-induced brain damage.

语种: 英语
所属项目编号: 30470555 ; 30870808 ; BK2007509 ; BK2010233
项目资助者: National Natural Science Foundation of China ; Natural Science Foundation of Jiangsu Province of China ; Saxon State Ministry of Social Affairs and Consumer Protection/Germany ; Health Department of the Jiangsu Province/China
WOS记录号: WOS:000302404000006
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/61439
Appears in Collections:北京大学第一临床医学院_儿科_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Soochow Univ, Affiliated Childrens Hosp, Neurol Lab, Suzhou 215003, Peoples R China
2.Soochow Univ, Inst Neurosci, Suzhou 215003, Peoples R China
3.Peking Univ, Hosp 1, Dept Pediat, Beijing 100034, Peoples R China

Recommended Citation:
Ni, Hong,Yan, Jian-zhen,Zhang, Le-ling,et al. Long-term Effects of Recurrent Neonatal Seizures on Neurobehavioral Function and Related Gene Expression and Its Intervention by Inhibitor of Cathepsin B[J]. NEUROCHEMICAL RESEARCH,2012,37(1):31-39.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Ni, Hong]'s Articles
[Yan, Jian-zhen]'s Articles
[Zhang, Le-ling]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Ni, Hong]‘s Articles
[Yan, Jian-zhen]‘s Articles
[Zhang, Le-ling]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace