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Induction of MIF expression by oxidized LDL via activation of NF-kappa B in vascular smooth muscle cells
Chen, Lihong1; Yang, Guangrui1; Zhang, Xiaoyan1; Wu, Jing1; Gu, Qing4; Wei, Mingfen1; Yang, Jichun1; Zhu, Yi1,3; Wang, Nanping2,3; Guan, Youfei1,3
关键词Atherosclerosis MIF NF-kappa B oxLDL VSMCs
刊名ATHEROSCLEROSIS
2009-12-01
DOI10.1016/j.atherosclerosis.2009.05.021
207期:2页:428-433
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Peripheral Vascular Disease
资助者Natural Science Foundation ; Ministry of Science and Technology ; Natural Science Foundation ; Ministry of Science and Technology
研究领域[WOS]Cardiovascular System & Cardiology
关键词[WOS]MIGRATION-INHIBITORY FACTOR ; LOW-DENSITY-LIPOPROTEIN ; HUMAN ATHEROSCLEROTIC PLAQUES ; ENDOTHELIAL-CELLS ; GENE-EXPRESSION ; DEFICIENT MICE ; ATHEROGENESIS ; DISEASE ; DESTABILIZATION ; MECHANISMS
英文摘要

Macrophage migration inhibitory factor (MIF) is a pleiotropic cytokine playing important roles in atherosclerosis. MIF gene deficiency and neutralizing antibodies against MIF have been reported to exert anti-atherosclerotic effects in various animal models. However, the mechanism by which MIF is induced in atherosclerotic lesions remains unclear. In the present studies, we cloned a 540 bp full-length rabbit MIF cDNA by screening a rabbit uterine library. The cDNA contains a 348 bp open-reading frame which encodes a deduced 115-amino acid polypeptide with similar to 90% similarity to human and mouse homologs. Constitutive MIF mRNA expression was detected in most rabbit tissues including aortas. The expression of MIF obviously abounded in vascular smooth muscle cells (VSMCs) of the atherosclerotic plaques. In cultured VSMCs, MIF expression was significantly induced by a pro-atherogenic factor, oxidized low-density lipoprotein (oxLDL). Promoter analysis showed there were two NF-kappa B binding sites in the MIF proximal promoter region. Deletion or mutation of the two sites abolished oxLDL-enhanced MIF promoter activity. Moreover, the induction of MIF by oxLDL can be blocked by I kappa B-alpha overexpression. Taken together, our results revealed that MIF expression can be induced by oxLDL in VSMCs via a NF-kappa B dependent manner, which may contribute to the pathogenesis of atherosclerosis. (C) 2009 Elsevier Ireland Ltd. All rights reserved.

语种英语
所属项目编号30725033 ; 30530340 ; 2006CB503907
资助者Natural Science Foundation ; Ministry of Science and Technology ; Natural Science Foundation ; Ministry of Science and Technology
WOS记录号WOS:000273566700021
引用统计
被引频次:26[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/61440
专题基础医学院_心血管所
作者单位1.Peking Univ, Hlth Sci Ctr, Dept Physiol & Pathophysiol, Beijing 100191, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Inst Cardiovasc Sci, Beijing 100191, Peoples R China
3.Peking Univ, Hlth Sci Ctr, Ctr Diabet, Beijing 100191, Peoples R China
4.Fuwai Hosp, Coronary Heart Dis Ctr, Beijing, Peoples R China
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GB/T 7714
Chen, Lihong,Yang, Guangrui,Zhang, Xiaoyan,et al. Induction of MIF expression by oxidized LDL via activation of NF-kappa B in vascular smooth muscle cells[J]. ATHEROSCLEROSIS,2009,207(2):428-433.
APA Chen, Lihong.,Yang, Guangrui.,Zhang, Xiaoyan.,Wu, Jing.,Gu, Qing.,...&Guan, Youfei.(2009).Induction of MIF expression by oxidized LDL via activation of NF-kappa B in vascular smooth muscle cells.ATHEROSCLEROSIS,207(2),428-433.
MLA Chen, Lihong,et al."Induction of MIF expression by oxidized LDL via activation of NF-kappa B in vascular smooth muscle cells".ATHEROSCLEROSIS 207.2(2009):428-433.
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