北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学第三临床医学院  > 心血管内科  > 期刊论文
学科主题: 临床医学
题名:
EFFECTS OF TEMPERATURE AND MOBILE PHASE COMPOSITION ON THE INTERACTION BETWEEN BERBERINE AND IMMOBILIZED beta(2)-ADRENOCEPTOR BY HIGH PERFORMANCE AFFINITY CHROMATOGRAPHY
作者: Zhao, Xinfeng1; Li, Qian1; Huang, Jingjing1; Zheng, Jianbin2; Zheng, Xiaohui1; Li, Zijian3,4; Zhang, Youyi3,4
关键词: (2)-adrenoceptor ; affinity chromatography ; association constant ; berberine ; binding site ; immobilized receptor
刊名: JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
发表日期: 2014-01-02
DOI: 10.1080/10826076.2012.734001
卷: 37, 期:1, 页:88-98
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]: HUMAN SERUM-ALBUMIN ; DIFFERENT CONFORMATIONAL STATES ; ACETYLCHOLINE-RECEPTORS ; IDENTIFICATION ; BINDING ; COLUMN
英文摘要:

Self-competitive displacement is used to examine changes in the association constant and the binding site for berberine binding to immobilized (2)-adrenoceptor ((2)-AR) at different temperatures and using a varying mobile phase. The compound was confirmed to have a single kind of binding site to (2)-AR under all the tested conditions. At 298.15K, the association constant and the binding site are (2.12 +/- 0.02)x10(4)M(-1) and (1.56 +/- 0.02)x10(2)M. Thermodynamic investigation indicates that the binding of berberine to (2)-AR has a positive change in energy due to enthalpy and negative response to entropy changes. Accordingly, this interaction is thought to be an endothermal process with entropy increase. The binding of berberine to the receptor was found to be more sensitive to ionic strength than pH. The placing of 1-propanol up to 5.0% in the mobile phases generates an 18% decrease in retention for berberine. These results indicate that the immobilized receptor would probably be an alternative for exploring the binding mechanism of ligand and receptor.

语种: 英语
所属项目编号: 21005060 ; IRT1174 ; 20106101110001 ; 2008BAI51B01 ; 11JK0661
项目资助者: National Natural Sciences Foundation of China ; Program for Changjiang Scholars and Innovative Research Team in University ; Chinese Ministry of Education ; Technology Support Plan Project ; Department of Education of Shaanxi Province
WOS记录号: WOS:000325785900007
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/61442
Appears in Collections:北京大学第三临床医学院_心血管内科_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Minist Educ, Key Lab Mol Cardiovasc Sci, Beijing, Peoples R China
2.NW Univ Xian, Coll Life Sci, Xian 710069, Peoples R China
3.NW Univ Xian, Inst Analyt Sci, Xian 710069, Peoples R China
4.Peking Univ, Hosp 3, Inst Vasc Med, Beijing 100871, Peoples R China

Recommended Citation:
Zhao, Xinfeng,Li, Qian,Huang, Jingjing,et al. EFFECTS OF TEMPERATURE AND MOBILE PHASE COMPOSITION ON THE INTERACTION BETWEEN BERBERINE AND IMMOBILIZED beta(2)-ADRENOCEPTOR BY HIGH PERFORMANCE AFFINITY CHROMATOGRAPHY[J]. JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,2014,37(1):88-98.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Zhao, Xinfeng]'s Articles
[Li, Qian]'s Articles
[Huang, Jingjing]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Zhao, Xinfeng]‘s Articles
[Li, Qian]‘s Articles
[Huang, Jingjing]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace