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EFFECTS OF TEMPERATURE AND MOBILE PHASE COMPOSITION ON THE INTERACTION BETWEEN BERBERINE AND IMMOBILIZED beta(2)-ADRENOCEPTOR BY HIGH PERFORMANCE AFFINITY CHROMATOGRAPHY
Zhao, Xinfeng1; Li, Qian1; Huang, Jingjing1; Zheng, Jianbin2; Zheng, Xiaohui1; Li, Zijian3,4; Zhang, Youyi3,4
关键词(2)-adrenoceptor affinity chromatography association constant berberine binding site immobilized receptor
刊名JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
2014-01-02
DOI10.1080/10826076.2012.734001
37期:1页:88-98
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]HUMAN SERUM-ALBUMIN ; DIFFERENT CONFORMATIONAL STATES ; ACETYLCHOLINE-RECEPTORS ; IDENTIFICATION ; BINDING ; COLUMN
英文摘要

Self-competitive displacement is used to examine changes in the association constant and the binding site for berberine binding to immobilized (2)-adrenoceptor ((2)-AR) at different temperatures and using a varying mobile phase. The compound was confirmed to have a single kind of binding site to (2)-AR under all the tested conditions. At 298.15K, the association constant and the binding site are (2.12 +/- 0.02)x10(4)M(-1) and (1.56 +/- 0.02)x10(2)M. Thermodynamic investigation indicates that the binding of berberine to (2)-AR has a positive change in energy due to enthalpy and negative response to entropy changes. Accordingly, this interaction is thought to be an endothermal process with entropy increase. The binding of berberine to the receptor was found to be more sensitive to ionic strength than pH. The placing of 1-propanol up to 5.0% in the mobile phases generates an 18% decrease in retention for berberine. These results indicate that the immobilized receptor would probably be an alternative for exploring the binding mechanism of ligand and receptor.

语种英语
WOS记录号WOS:000325785900007
引用统计
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/61442
专题北京大学第三临床医学院_心血管内科
作者单位1.Minist Educ, Key Lab Mol Cardiovasc Sci, Beijing, Peoples R China
2.NW Univ Xian, Coll Life Sci, Xian 710069, Peoples R China
3.NW Univ Xian, Inst Analyt Sci, Xian 710069, Peoples R China
4.Peking Univ, Hosp 3, Inst Vasc Med, Beijing 100871, Peoples R China
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GB/T 7714
Zhao, Xinfeng,Li, Qian,Huang, Jingjing,et al. EFFECTS OF TEMPERATURE AND MOBILE PHASE COMPOSITION ON THE INTERACTION BETWEEN BERBERINE AND IMMOBILIZED beta(2)-ADRENOCEPTOR BY HIGH PERFORMANCE AFFINITY CHROMATOGRAPHY[J]. JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,2014,37(1):88-98.
APA Zhao, Xinfeng.,Li, Qian.,Huang, Jingjing.,Zheng, Jianbin.,Zheng, Xiaohui.,...&Zhang, Youyi.(2014).EFFECTS OF TEMPERATURE AND MOBILE PHASE COMPOSITION ON THE INTERACTION BETWEEN BERBERINE AND IMMOBILIZED beta(2)-ADRENOCEPTOR BY HIGH PERFORMANCE AFFINITY CHROMATOGRAPHY.JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,37(1),88-98.
MLA Zhao, Xinfeng,et al."EFFECTS OF TEMPERATURE AND MOBILE PHASE COMPOSITION ON THE INTERACTION BETWEEN BERBERINE AND IMMOBILIZED beta(2)-ADRENOCEPTOR BY HIGH PERFORMANCE AFFINITY CHROMATOGRAPHY".JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES 37.1(2014):88-98.
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